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- PDB-4lz9: Structure of MATE multidrug transporter DinF-BH in complex with R6G -
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Open data
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Basic information
Entry | Database: PDB / ID: 4lz9 | ||||||
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Title | Structure of MATE multidrug transporter DinF-BH in complex with R6G | ||||||
![]() | BH2163 protein | ||||||
![]() | TRANSPORT PROTEIN / multidrug transporter | ||||||
Function / homology | ![]() antiporter activity / xenobiotic transmembrane transporter activity / response to antibiotic / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Lu, M. / Radchenko, M. / Symersky, J. / Nie, R. / Guo, Y. | ||||||
![]() | ![]() Title: Structural insights into H(+)-coupled multidrug extrusion by a MATE transporter Authors: Lu, M. / Radchenko, M. / Symersky, J. / Nie, R. / Guo, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 29.5 KB | Display | ![]() |
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PDB format | ![]() | 15 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 569.9 KB | Display | ![]() |
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Full document | ![]() | 572 KB | Display | |
Data in XML | ![]() | 2.6 KB | Display | |
Data in CIF | ![]() | 6.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 48615.172 Da / Num. of mol.: 1 / Fragment: UNP residues 3-448 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-RHQ / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.45 Å3/Da / Density % sol: 72.38 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / pH: 8.5 Details: PEG, NaCl, pH 8.5, VAPOR DIFFUSION, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jan 1, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.7→60 Å / Num. all: 9715 / Num. obs: 9220 / % possible obs: 95 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Rsym value: 0.066 |
Reflection shell | Highest resolution: 3.7 Å / % possible all: 95 |
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Processing
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Refinement | Method to determine structure: AB INITIO PHASING / Resolution: 3.7→20 Å / σ(F): 2 / σ(I): 2 / Stereochemistry target values: Engh & Huber / Details: The coordinates contain only a CA trace.
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Refinement step | Cycle: LAST / Resolution: 3.7→20 Å
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Refine LS restraints |
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