+Open data
-Basic information
Entry | Database: PDB / ID: 4lz6 | ||||||
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Title | Structure of MATE multidrug transporter DinF-BH | ||||||
Components | BH2163 protein | ||||||
Keywords | TRANSPORT PROTEIN / multidrug transporter | ||||||
Function / homology | Multidrug and toxic compound extrusion MepA-like / : / Multi antimicrobial extrusion protein / MatE / antiporter activity / xenobiotic transmembrane transporter activity / response to antibiotic / plasma membrane / Multidrug export protein MepA Function and homology information | ||||||
Biological species | Bacillus halodurans (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / AB INITIO PHASING / Resolution: 3.2 Å | ||||||
Authors | Lu, M. / Radchenko, M. / Symersky, J. / Nie, R. / Guo, Y. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2013 Title: Structural insights into H(+)-coupled multidrug extrusion by a MATE transporter Authors: Lu, M. / Radchenko, M. / Symersky, J. / Nie, R. / Guo, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4lz6.cif.gz | 97.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4lz6.ent.gz | 74.5 KB | Display | PDB format |
PDBx/mmJSON format | 4lz6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lz/4lz6 ftp://data.pdbj.org/pub/pdb/validation_reports/lz/4lz6 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 48615.172 Da / Num. of mol.: 1 / Fragment: UNP residues 3-448 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus halodurans (bacteria) / Strain: C-125 / Gene: BH2163 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9KAX3 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.51 Å3/Da / Density % sol: 72.73 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / pH: 8.5 Details: PEG, NaCl, pH 8.5, VAPOR DIFFUSION, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jan 1, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→60 Å / Num. obs: 14873 / % possible obs: 97.3 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Rsym value: 0.056 |
Reflection shell | Highest resolution: 3.2 Å / Redundancy: 15 % / Mean I/σ(I) obs: 58.5 / Num. unique all: 14873 / Rsym value: 0.056 / % possible all: 97.3 |
-Processing
Software |
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Refinement | Method to determine structure: AB INITIO PHASING / Resolution: 3.2→15 Å / σ(F): 2 / σ(I): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 3.2→15 Å
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Refine LS restraints |
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LS refinement shell | Highest resolution: 3.2 Å
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