+
データを開く
-
基本情報
登録情報 | データベース: PDB / ID: 4lwd | ||||||
---|---|---|---|---|---|---|---|
タイトル | Human CARMA1 CARD domain | ||||||
![]() | Caspase recruitment domain-containing protein 11 | ||||||
![]() | SIGNALING PROTEIN / death domain / Bcl10 | ||||||
機能・相同性 | ![]() thymic T cell selection / CBM complex / regulation of B cell differentiation / : / guanylate kinase activity / CD4-positive, alpha-beta T cell proliferation / TORC1 signaling / CARD domain binding / positive regulation of CD4-positive, alpha-beta T cell proliferation / regulation of T cell differentiation ...thymic T cell selection / CBM complex / regulation of B cell differentiation / : / guanylate kinase activity / CD4-positive, alpha-beta T cell proliferation / TORC1 signaling / CARD domain binding / positive regulation of CD4-positive, alpha-beta T cell proliferation / regulation of T cell differentiation / positive regulation of T cell receptor signaling pathway / B cell proliferation / immunological synapse / homeostasis of number of cells / canonical NF-kappaB signal transduction / positive regulation of B cell proliferation / T cell costimulation / positive regulation of interleukin-2 production / B cell differentiation / Activation of NF-kappaB in B cells / protein homooligomerization / CLEC7A (Dectin-1) signaling / FCERI mediated NF-kB activation / Downstream TCR signaling / positive regulation of NF-kappaB transcription factor activity / regulation of apoptotic process / positive regulation of canonical NF-kappaB signal transduction / membrane raft / extracellular exosome / plasma membrane / cytosol / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() | ||||||
![]() | Zheng, C. / Wu, H. | ||||||
![]() | ![]() タイトル: Structural architecture of the CARMA1/Bcl10/MALT1 signalosome: nucleation-induced filamentous assembly. 著者: Qi Qiao / Chenghua Yang / Chao Zheng / Lorena Fontán / Liron David / Xiong Yu / Clay Bracken / Monica Rosen / Ari Melnick / Edward H Egelman / Hao Wu / ![]() 要旨: The CARMA1/Bcl10/MALT1 (CBM) signalosome mediates antigen receptor-induced NF-κB signaling to regulate multiple lymphocyte functions. While CARMA1 and Bcl10 contain caspase recruitment domains ...The CARMA1/Bcl10/MALT1 (CBM) signalosome mediates antigen receptor-induced NF-κB signaling to regulate multiple lymphocyte functions. While CARMA1 and Bcl10 contain caspase recruitment domains (CARDs), MALT1 is a paracaspase with structural similarity to caspases. Here we show that the reconstituted CBM signalosome is a helical filamentous assembly in which substoichiometric CARMA1 nucleates Bcl10 filaments. Bcl10 filament formation is a highly cooperative process whose threshold is sensitized by oligomerized CARMA1 upon receptor activation. In cells, both cotransfected CARMA1/Bcl10 complex and the endogenous CBM signalosome are filamentous morphologically. Combining crystallography, nuclear magnetic resonance, and electron microscopy, we reveal the structure of the Bcl10 CARD filament and the mode of interaction between CARMA1 and Bcl10. Structure-guided mutagenesis confirmed the observed interfaces in Bcl10 filament assembly and MALT1 activation in vitro and NF-κB activation in cells. These data support a paradigm of nucleation-induced signal transduction with threshold response due to cooperativity and signal amplification by polymerization. | ||||||
履歴 |
|
-
構造の表示
構造ビューア | 分子: ![]() ![]() |
---|
-
ダウンロードとリンク
-
ダウンロード
PDBx/mmCIF形式 | ![]() | 53 KB | 表示 | ![]() |
---|---|---|---|---|
PDB形式 | ![]() | 39.1 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
---|
-関連構造データ
-
リンク
-
集合体
登録構造単位 | ![]()
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||||
2 | ![]()
| x 6||||||||||||
単位格子 |
| ||||||||||||
Components on special symmetry positions |
|
-
要素
#1: タンパク質 | 分子量: 11385.998 Da / 分子数: 1 / 断片: CARD domain residues 18-110 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() | ||||
---|---|---|---|---|---|
#2: 化合物 | #3: 化合物 | ChemComp-MG / | #4: 水 | ChemComp-HOH / | |
-実験情報
-実験
実験 | 手法: ![]() |
---|
-
試料調製
結晶 | マシュー密度: 2.02 Å3/Da / 溶媒含有率: 39.14 % |
---|---|
結晶化 | 温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 6.5 詳細: It was crystallized by mixing 1 to l protein (10 mg/ml in 20 mM Tris at pH 8.0, 150 mM NaCl, and 5 mM DTT) with 1 to l of the reservoir solution containing 1.4 M MgSO4 and 100 mM MES at pH 6. ...詳細: It was crystallized by mixing 1 to l protein (10 mg/ml in 20 mM Tris at pH 8.0, 150 mM NaCl, and 5 mM DTT) with 1 to l of the reservoir solution containing 1.4 M MgSO4 and 100 mM MES at pH 6.5 in a hanging drop vapor diffusion system at 20 oC., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-データ収集
回折 | 平均測定温度: 100 K | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
放射光源 | 由来: ![]() | ||||||||||||
検出器 | タイプ: RIGAKU RAXIS IV / 検出器: IMAGE PLATE / 日付: 2013年5月13日 | ||||||||||||
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray | ||||||||||||
放射波長 | 波長: 1.5418 Å / 相対比: 1 | ||||||||||||
反射 | 解像度: 1.792→25.945 Å / Num. obs: 9328 / Observed criterion σ(F): 1.36 / Observed criterion σ(I): 2 | ||||||||||||
反射 シェル |
|
-
解析
ソフトウェア |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
精密化 | 構造決定の手法: ![]()
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL / Bsol: 55.146 Å2 / ksol: 0.449 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 1.792→25.945 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS精密化 シェル |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 TLS | 手法: refined / Refine-ID: X-RAY DIFFRACTION
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 TLSグループ |
|