温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 8.4 詳細: deoxy-HbTb crystals have been grown using 8-9.5 mg/ml protein mixed with equal amount of 7-12 % w/v PEG 6000, PBS 100 mM, KCl 0.3 M, pH 8.4. Deoxy HbTb crystals were then soaked for several ...詳細: deoxy-HbTb crystals have been grown using 8-9.5 mg/ml protein mixed with equal amount of 7-12 % w/v PEG 6000, PBS 100 mM, KCl 0.3 M, pH 8.4. Deoxy HbTb crystals were then soaked for several hours in a deoxygenated, CO saturated, stabilizing solution containing 30% w/v PEG 6000, 100 mM Trsi-HCl pH 8.4, 200 mM KCl, 5 mM dithionite reducing agent and flushed with gaseous CO before data collection, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
構造決定の手法: フーリエ合成 / 解像度: 2.2→72.165 Å / 交差検証法: THROUGHOUT / σ(F): 2 / 立体化学のターゲット値: Engh & Huber 詳細: DIFFRACTION DATA WERE COLLEXCTED ON TWO SINGLE CRYSTALS. THE TWO DATA SET WERE INDEXED, PROCESSED AND SCALED WITH HKL2000. THE AGREEMENT BETWEEN THE TWO DATA SETS WAS OF THE ORDER OF THE ...詳細: DIFFRACTION DATA WERE COLLEXCTED ON TWO SINGLE CRYSTALS. THE TWO DATA SET WERE INDEXED, PROCESSED AND SCALED WITH HKL2000. THE AGREEMENT BETWEEN THE TWO DATA SETS WAS OF THE ORDER OF THE EXPECTED ERRORS (RMERGE 0.069). FOR EACH DATA SET, THE STRUCTURE WAS REFINED WITH SHELX PROGRAM. SINCE ALL THE BASIC FEATURES, SUCH AS THE COORDINATION AT THE HEME IRONS, SUBSTANTIALLY AGREE IN THE TWO STRUCTURES THE TWO DATA SETS WERE MERGED FOR SUCCESSIVE REFINEMENT STEPS. ALL REFLECTION WERE USED FOR REFINEMENT