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- PDB-4gk1: Crystal structure of CD23 lectin domain mutant D270A -

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Basic information

Entry
Database: PDB / ID: 4gk1
TitleCrystal structure of CD23 lectin domain mutant D270A
ComponentsLow affinity immunoglobulin epsilon Fc receptor
KeywordsIMMUNE SYSTEM / Receptor
Function / homology
Function and homology information


low-affinity IgE receptor activity / B cell antigen processing and presentation / Fc receptor-mediated immune complex endocytosis / positive regulation of humoral immune response mediated by circulating immunoglobulin / NOTCH2 intracellular domain regulates transcription / macrophage activation / IgE binding / positive regulation of killing of cells of another organism / positive regulation of nitric-oxide synthase biosynthetic process / Interleukin-10 signaling ...low-affinity IgE receptor activity / B cell antigen processing and presentation / Fc receptor-mediated immune complex endocytosis / positive regulation of humoral immune response mediated by circulating immunoglobulin / NOTCH2 intracellular domain regulates transcription / macrophage activation / IgE binding / positive regulation of killing of cells of another organism / positive regulation of nitric-oxide synthase biosynthetic process / Interleukin-10 signaling / positive regulation of nitric-oxide synthase activity / integrin binding / carbohydrate binding / Interleukin-4 and Interleukin-13 signaling / immune response / external side of plasma membrane / extracellular exosome / metal ion binding / plasma membrane
Similarity search - Function
CD209-like, C-type lectin-like domain / C-type lectin, conserved site / C-type lectin domain signature. / Mannose-Binding Protein A; Chain A / Mannose-Binding Protein A, subunit A / Lectin C-type domain / C-type lectin domain profile. / C-type lectin-like / C-type lectin (CTL) or carbohydrate-recognition domain (CRD) / C-type lectin-like/link domain superfamily ...CD209-like, C-type lectin-like domain / C-type lectin, conserved site / C-type lectin domain signature. / Mannose-Binding Protein A; Chain A / Mannose-Binding Protein A, subunit A / Lectin C-type domain / C-type lectin domain profile. / C-type lectin-like / C-type lectin (CTL) or carbohydrate-recognition domain (CRD) / C-type lectin-like/link domain superfamily / C-type lectin fold / Roll / Alpha Beta
Similarity search - Domain/homology
Low affinity immunoglobulin epsilon Fc receptor
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.236 Å
AuthorsYuan, D. / Sutton, B.J. / Dhaliwal, B.
CitationJournal: J.Biol.Chem. / Year: 2013
Title: Ca2+-dependent Structural Changes in the B-cell Receptor CD23 Increase Its Affinity for Human Immunoglobulin E.
Authors: Yuan, D. / Keeble, A.H. / Hibbert, R.G. / Fabiane, S. / Gould, H.J. / McDonnell, J.M. / Beavil, A.J. / Sutton, B.J. / Dhaliwal, B.
History
DepositionAug 10, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 26, 2013Provider: repository / Type: Initial release
Revision 1.1Aug 14, 2013Group: Database references
Revision 1.2Jul 17, 2019Group: Data collection / Refinement description / Category: software
Item: _software.contact_author / _software.contact_author_email ..._software.contact_author / _software.contact_author_email / _software.language / _software.location / _software.name / _software.type / _software.version
Revision 1.3Sep 13, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Low affinity immunoglobulin epsilon Fc receptor
B: Low affinity immunoglobulin epsilon Fc receptor
C: Low affinity immunoglobulin epsilon Fc receptor
D: Low affinity immunoglobulin epsilon Fc receptor
E: Low affinity immunoglobulin epsilon Fc receptor
F: Low affinity immunoglobulin epsilon Fc receptor
G: Low affinity immunoglobulin epsilon Fc receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)116,35444
Polymers112,8477
Non-polymers3,50737
Water8,485471
1
A: Low affinity immunoglobulin epsilon Fc receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,5936
Polymers16,1211
Non-polymers4725
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
2
B: Low affinity immunoglobulin epsilon Fc receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,6937
Polymers16,1211
Non-polymers5726
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
3
C: Low affinity immunoglobulin epsilon Fc receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,6897
Polymers16,1211
Non-polymers5686
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
4
D: Low affinity immunoglobulin epsilon Fc receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,4975
Polymers16,1211
Non-polymers3764
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
5
E: Low affinity immunoglobulin epsilon Fc receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,5976
Polymers16,1211
Non-polymers4765
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
6
F: Low affinity immunoglobulin epsilon Fc receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,97410
Polymers16,1211
Non-polymers8539
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
7
G: Low affinity immunoglobulin epsilon Fc receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,3093
Polymers16,1211
Non-polymers1882
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)73.776, 109.106, 139.001
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein
Low affinity immunoglobulin epsilon Fc receptor / BLAST-2 / C-type lectin domain family 4 member J / Fc-epsilon-RII / Immunoglobulin E-binding factor ...BLAST-2 / C-type lectin domain family 4 member J / Fc-epsilon-RII / Immunoglobulin E-binding factor / Lymphocyte IgE receptor / Low affinity immunoglobulin epsilon Fc receptor membrane-bound form / Low affinity immunoglobulin epsilon Fc receptor soluble form


Mass: 16120.977 Da / Num. of mol.: 7 / Fragment: UNP residues 156-298 / Mutation: D270A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FCER2, CD23A, CLEC4J, FCE2, IGEBF / Production host: Escherichia coli (E. coli) / References: UniProt: P06734
#2: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: C3H8O3
#3: Chemical...
ChemComp-SO4 / SULFATE ION / Sulfate


Mass: 96.063 Da / Num. of mol.: 25 / Source method: obtained synthetically / Formula: SO4
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 471 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.48 Å3/Da / Density % sol: 50.38 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 5.5
Details: 30% PEG 4000, 0.3M ammonium sulphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I02 / Wavelength: 0.9795 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 10, 2010
RadiationMonochromator: Double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9795 Å / Relative weight: 1
ReflectionResolution: 2.236→50 Å / Num. all: 54765 / Num. obs: 54601 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0
Reflection shellResolution: 2.236→2.33 Å / % possible all: 99.5

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Processing

Software
NameVersionClassificationNB
PHENIX1.7_650refinement
PDB_EXTRACT3.11data extraction
REFMACrefinement
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 2H2R

2h2r


Resolution: 2.236→43.864 Å / Occupancy max: 1 / Occupancy min: 0.5 / SU ML: 0.3 / σ(F): 1.34 / Phase error: 23.38 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2256 2768 5.08 %random
Rwork0.1809 ---
all0.1832 54765 --
obs0.1832 54533 99.22 %-
Solvent computationShrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 50.887 Å2 / ksol: 0.359 e/Å3
Displacement parametersBiso max: 261.46 Å2 / Biso mean: 44.2896 Å2 / Biso min: 15.49 Å2
Baniso -1Baniso -2Baniso -3
1-9.8846 Å2-0 Å2-0 Å2
2---2.3416 Å20 Å2
3----7.543 Å2
Refinement stepCycle: LAST / Resolution: 2.236→43.864 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7441 0 197 471 8109
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONf_bond_d0.006
X-RAY DIFFRACTIONf_angle_deg0.937
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.74540.0957-0.42650.3496-0.19710.3165-0.0062-0.3982-0.04860.2669-0.22410.0995-0.00830.26830.09870.4508-0.18890.05540.9342-0.13030.3693-25.848914.362345.7399
20.59040.21370.03530.1611-0.04160.01920.179-0.2596-0.04150.1186-0.1003-0.0359-0.1408-0.04860.03570.2866-0.18260.01870.3739-0.10090.2697-19.550215.096435.9753
30.04160.005-0.03020.9186-0.2950.31270.06550.0645-0.23470.1302-0.2837-0.42490.09630.23720.08150.1925-0.0741-0.03030.4829-0.02910.3263-10.83086.974132.6153
40.2457-0.004-0.03250.07780.0040.0830.1907-0.1499-0.09910.2813-0.17860.0220.0246-0.0014-0.00810.3121-0.102-0.02870.254-0.04070.2758-22.55238.587532.5501
50.2826-0.06750.1620.0331-0.01980.12760.26860.0580.1614-0.1025-0.021-0.0136-0.17070.0291-0.10990.3653-0.07650.10650.1689-0.04870.3377-27.373619.803831.4506
62.4740.6397-0.51030.61610.3141.29340.0933-0.13710.3176-0.0849-0.0134-0.0618-0.30360.1529-0.0120.2861-0.1074-0.00490.2815-0.01910.3845-16.205218.482326.5202
71.1169-0.0096-0.27891.2816-0.45970.5268-0.19990.2105-0.2446-0.04430.064-0.50390.05860.00830.04710.2438-0.02380.04470.2596-0.06810.3782-18.68250.609818.0045
80.60630.02260.07410.13010.08820.44950.1710.13930.0065-0.1099-0.0117-0.15270.08-0.0293-0.04190.2685-0.0060.03940.2205-0.00740.2474-25.82928.254621.3967
90.070.0132-0.01990.0121-0.00720.0872-0.03560.029-0.0212-0.03450.0063-0.02280.14930.0330.03210.2413-0.04120.06670.2165-0.01420.2307-17.32410.431315.3439
100.00120.0070.00120.18550.06760.16980.0101-0.00410.2534-0.06330.04680.07350.0576-0.0882-0.02110.1471-0.05010.04470.1328-0.00330.3188-20.968518.092320.021
110.02590.05-0.02410.15660.03720.11840.03340.05030.00160.06520.0883-0.0230.05080.0437-0.0150.2094-0.11910.00460.30640.01860.4332-8.484413.067920.7492
120.7716-0.0114-0.37441.028-0.08280.39560.2201-0.51470.04560.04360.0896-0.21990.1648-0.154-0.18120.2486-0.12980.0070.5341-0.02990.3213-20.438411.024138.7197
130.1292-0.1971-0.32491.66190.49650.96870.1667-0.2795-0.20580.2464-0.18020.46070.0775-0.44570.08790.2359-0.09140.01090.4245-0.01250.2218-8.3927-10.720359.1135
140.9719-0.5975-0.03151.533-0.95611.3470.481-0.1152-0.03050.0432-0.0660.2754-0.0445-0.1541-0.25010.2299-0.0660.00050.328-0.0110.2789-14.6546-11.434149.4874
151.1454-0.62920.51822.6743-1.84731.28780.19850.30610.1984-0.3699-0.0130.0217-0.2404-0.0163-0.05150.61780.10230.09510.37040.04530.342-19.5721-1.484443.9741
160.72270.3050.50050.4651-0.2070.85090.0543-0.13690.05150.2404-0.0922-0.0826-0.290.0520.03810.3773-0.0898-0.04340.3544-0.08570.2868-9.4454-7.727945.0797
171.1287-1.15660.12881.2376-0.31080.94030.3377-0.0244-0.4508-0.17460.06870.40170.17620.0315-0.20250.3511-0.0766-0.05650.2779-0.02230.3613-9.3753-20.004346.5503
180.2749-0.01950.02721.0365-0.0310.11760.14820.0409-0.1997-0.04610.2517-0.01820.0558-0.0115-0.19390.3331-0.0473-0.09020.3941-0.01860.2952-19.3531-15.230840.9661
190.28450.07990.1090.04910.08740.2310.35880.05630.0819-0.1383-0.0964-0.03750.00480.0198-0.0850.52360.0640.05190.251-0.01490.2684-7.641-4.053732.8214
200.2603-0.12340.06460.0505-0.04720.02960.37220.3566-0.2692-0.3043-0.16890.05030.0406-0.27830.3520.37020.0359-0.21640.3808-0.4922-0.5984-11.5341-13.184130.2387
210.19020.1746-0.03460.90210.22870.36570.24120.1322-0.1453-0.03190.14210.04130.34650.0502-0.14980.38790.0201-0.16690.3125-0.06710.3722-14.6026-18.265934.7471
220.141-0.14590.3710.8163-0.4071.0050.33960.1437-0.0905-0.1163-0.25380.06740.13840.0215-0.00160.23850.0454-0.04560.54420.05920.2503-24.4142-8.186933.1741
230.39510.06260.70250.24210.24191.33410.10780.1003-0.058-0.2060.08280.0165-0.17120.1335-0.07690.3225-0.01520.01980.2476-0.0140.1878-11.5327-7.095652.3551
240.2731-0.11360.16730.1520.13840.5453-0.0588-0.09890.0042-0.0271-0.10030.0984-0.1117-0.6153-0.03660.14160.0635-0.00310.38480.01460.2728-44.68038.324820.8038
250.1089-0.08970.29331.1828-0.26481.08730.2750.0915-0.0081-0.0975-0.05870.2844-0.114-0.49770.06070.1830.02230.05380.3419-0.00070.3605-51.07518.45930.4247
260.12550.0351-0.15530.545-0.08590.2285-0.24430.037-0.21210.2394-0.08580.04490.2833-0.11980.11440.5016-0.14330.10660.3735-0.06550.3459-55.8806-1.706735.7942
270.27890.1925-0.03891.1446-0.58450.38910.1114-0.01930.2105-0.165-0.00380.17910.00450.07150.22090.21960.00240.07930.2157-0.07630.2627-48.706211.699235.5759
280.41830.23670.1540.16220.00930.36870.11820.0831-0.23650.1892-0.0335-0.31640.261-0.0146-0.0420.3257-0.0182-0.01450.1955-0.0710.3485-44.40.605347.2105
290.19940.21190.03220.3721-0.03740.68240.0948-0.03990.11640.114-0.03810.1409-0.08070.0747-0.03760.5241-0.07750.11620.3079-0.12650.2982-43.632313.137647.1163
301.63890.18910.80050.87480.39550.51140.1149-0.54630.07410.166-0.08990.1030.0549-0.41850.0390.3056-0.04870.05070.4041-0.12290.27-51.94037.368151.887
310.5905-0.48930.18320.59620.41721.70510.1631-0.18670.28970.15950.11120.0932-0.5336-0.2814-0.13920.3322-0.00210.15820.2888-0.02630.3045-55.806310.574845.623
320.45550.2319-0.26660.452-0.19530.426-0.02290.03530.0362-0.04550.13580.03090.0146-0.0279-0.04290.2282-0.03340.01920.3152-0.03880.2289-47.27464.298626.1825
330.3115-0.086-0.10990.04460.05680.0790.140.0047-0.28590.3557-0.14040.13820.2644-0.0258-0.10110.4911-0.1467-0.10170.24190.0090.3274-43.9433-26.635212.1376
340.2478-0.0159-0.2470.0886-0.04010.28560.0089-0.0665-0.20730.06590.1247-0.24320.37040.1275-0.03980.43260.0402-0.05970.16070.00970.331-30.1637-24.755816.488
351.20570.6375-0.02930.572-0.54721.3161-0.06330.0777-0.24760.1531-0.14860.08180.4817-0.1583-0.20060.2832-0.1053-0.01050.1661-0.02640.212-43.2313-17.653615.908
361.08550.5432-0.55120.3847-0.08640.5911-0.0785-0.1211-0.0466-0.00880.00920.0267-0.01340.11940.01430.1861-0.0041-0.01760.2752-0.06170.2819-27.168-8.908814.055
370.7679-0.2160.49260.3096-0.40540.66340.06560.06030.2188-0.0104-0.19580.02950.08530.089-0.01820.2151-0.0479-0.02790.2032-0.01580.1951-35.5791-7.793217.5902
381.19750.50350.09720.64460.39160.3040.0992-0.31370.01740.2823-0.18620.16950.1288-0.28660.03570.2661-0.04670.01820.2653-0.02140.1911-39.8201-10.361123.405
390.56210.19960.25110.46850.08070.10780.0619-0.1826-0.12020.11940.0204-0.0932-0.2444-0.05280.01250.45-0.0532-0.04070.21060.02410.2335-38.2751-23.884114.6362
400.5542-0.3163-0.61691.48040.15720.74030.29890.5415-0.0175-0.3584-0.1307-0.35750.30630.0325-0.10930.35320.06280.00610.3075-0.02920.3237-59.391-18.420440.4469
410.2134-0.2254-0.20010.46240.26920.20480.02720.02690.07920.02620.095-0.6205-0.0480.1453-0.08890.22630.01290.03740.2537-0.07520.4369-48.146-10.558947.8221
420.3688-0.2685-0.37110.29810.10850.58050.02820.02130.1529-0.09190.0176-0.2859-0.01290.1969-0.08290.2428-0.00450.02060.302-0.07250.3412-59.8999-11.956247.6633
431.86570.9987-0.25141.0008-0.40970.7854-0.0134-0.0978-0.2933-0.1238-0.03910.05630.2972-0.1873-0.00620.2927-0.0582-0.06640.1886-0.0230.2996-65.2175-22.872549.3311
441.36610.22760.43321.97340.62880.9961-0.1268-0.02190.0320.0587-0.04740.19350.29250.18780.10910.27440.08330.03560.23480.00270.3129-53.7915-21.810954.1228
450.45750.61390.28020.93770.28810.2372-0.1016-0.16990.19710.0115-0.055-0.0954-0.1489-0.11620.06930.29540.0347-0.06360.2718-0.10780.4072-56.1974-3.327361.7152
460.95870.23440.35390.74340.37760.48920.1-0.49870.16710.3916-0.0226-0.21830.0918-0.1413-0.03310.3047-0.0242-0.0440.3421-0.04970.2526-60.0121-11.942561.213
470.86030.41030.36721.04210.25990.56630.0105-0.07-0.02460.19410.01810.0080.04310.1126-0.01770.3119-0.0632-0.06370.30630.02070.3831-57.4221-19.998861.2261
480.42160.13520.01070.55320.17731.01720.03940.1486-0.1838-0.09220.0044-0.355-0.1582-0.1087-0.09230.24790.1066-0.01860.25650.01180.3323-53.9036-15.619546.844
490.6652-0.19010.4040.2704-0.52761.30680.21840.0757-0.2114-0.3548-0.1822-0.02820.66630.1814-0.06660.39150.0468-0.06250.2350.00970.2813-32.9548-21.107-7.3425
501.1074-0.4008-0.57390.4307-0.05170.5317-0.26650.40730.0949-0.00350.0017-0.1262-0.0523-0.06770.06090.2221-0.0378-0.04920.40110.06280.2216-46.5324-7.2279-4.8511
511.08320.05870.15380.29790.26660.49690.03850.03390.3027-0.0088-0.09880.0260.12060.0196-0.00970.2157-0.0204-0.04310.24680.03880.2052-37.5944-4.9605-7.0159
520.64070.07910.6570.8659-0.09770.89790.00260.28250.2428-0.3436-0.16220.10420.20270.11320.06880.25380.0297-0.03630.25690.0520.2122-36.2735-8.4538-14.0854
530.6513-0.1534-0.22220.10590.02230.0985-0.20130.1108-0.1791-0.0348-0.00620.0826-0.00590.08430.08010.4633-0.0024-0.08370.2569-0.00220.2905-35.0939-22.9632-6.8339
540.4409-0.0347-0.25210.03360.15280.8308-0.0134-0.12190.07130.01140.1616-0.2678-0.33980.6135-0.0860.5128-0.1180.14320.6086-0.07540.50173.54375.74229.4805
550.19750.1591-0.17350.1723-0.21260.28410.00290.21830.0399-0.03190.07150.05120.0114-0.18890.04780.2201-0.02640.10530.3642-0.10580.4116-8.9654-1.089410.4304
560.89480.0694-0.57570.20020.26960.7434-0.2054-0.1494-0.2502-0.13390.0621-0.2180.07320.38320.04580.4006-0.08140.13890.3699-0.04330.4813.7976-0.85172.6128
570.60550.26250.08990.18440.06590.1014-0.15340.28230.0188-0.1550.1445-0.00960.09450.04040.00660.8154-0.3765-0.03150.6917-0.03580.4168-11.3105-4.4419-5.3357
580.05780.08140.09620.09710.14510.2491-0.26130.2609-0.08020.09030.16990.04360.36680.07630.09620.7064-0.2320.18960.4459-0.09620.5869-3.6801-7.8523-5.6053
590.3337-0.59890.5521.096-0.8053.3839-0.06190.0279-0.0235-0.1053-0.2106-0.37780.26550.59810.12310.6478-0.08450.33430.6667-0.12360.60943.3321-10.1881-1.7647
600.26920.1040.11090.8143-0.2350.1539-0.0160.0838-0.02580.03780.0802-0.04520.1877-0.04480.01690.9934-0.01580.21880.31610.02680.5037-6.7587-14.1967.1545
612.5284-1.09170.35110.66010.52552.5205-0.1456-0.13450.3023-0.2332-0.067-0.055-0.48450.16150.04870.5104-0.09160.14240.4189-0.06030.53860.19336.74088.2308
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'B' and (resseq 156:167)B156 - 167
2X-RAY DIFFRACTION2chain 'B' and (resseq 168:183)B168 - 183
3X-RAY DIFFRACTION3chain 'B' and (resseq 184:194)B184 - 194
4X-RAY DIFFRACTION4chain 'B' and (resseq 195:203)B195 - 203
5X-RAY DIFFRACTION5chain 'B' and (resseq 204:213)B204 - 213
6X-RAY DIFFRACTION6chain 'B' and (resseq 214:222)B214 - 222
7X-RAY DIFFRACTION7chain 'B' and (resseq 223:230)B223 - 230
8X-RAY DIFFRACTION8chain 'B' and (resseq 231:249)B231 - 249
9X-RAY DIFFRACTION9chain 'B' and (resseq 250:262)B250 - 262
10X-RAY DIFFRACTION10chain 'B' and (resseq 263:271)B263 - 271
11X-RAY DIFFRACTION11chain 'B' and (resseq 272:278)B272 - 278
12X-RAY DIFFRACTION12chain 'B' and (resseq 279:288)B279 - 288
13X-RAY DIFFRACTION13chain 'A' and (resseq 156:167)A156 - 167
14X-RAY DIFFRACTION14chain 'A' and (resseq 168:183)A168 - 183
15X-RAY DIFFRACTION15chain 'A' and (resseq 184:194)A184 - 194
16X-RAY DIFFRACTION16chain 'A' and (resseq 195:203)A195 - 203
17X-RAY DIFFRACTION17chain 'A' and (resseq 204:213)A204 - 213
18X-RAY DIFFRACTION18chain 'A' and (resseq 214:222)A214 - 222
19X-RAY DIFFRACTION19chain 'A' and (resseq 223:239)A223 - 239
20X-RAY DIFFRACTION20chain 'A' and (resseq 240:262)A240 - 262
21X-RAY DIFFRACTION21chain 'A' and (resseq 263:271)A263 - 271
22X-RAY DIFFRACTION22chain 'A' and (resseq 272:278)A272 - 278
23X-RAY DIFFRACTION23chain 'A' and (resseq 279:289)A279 - 289
24X-RAY DIFFRACTION24chain 'C' and (resseq 156:167)C156 - 167
25X-RAY DIFFRACTION25chain 'C' and (resseq 168:183)C168 - 183
26X-RAY DIFFRACTION26chain 'C' and (resseq 184:194)C184 - 194
27X-RAY DIFFRACTION27chain 'C' and (resseq 195:222)C195 - 222
28X-RAY DIFFRACTION28chain 'C' and (resseq 223:239)C223 - 239
29X-RAY DIFFRACTION29chain 'C' and (resseq 240:249)C240 - 249
30X-RAY DIFFRACTION30chain 'C' and (resseq 250:262)C250 - 262
31X-RAY DIFFRACTION31chain 'C' and (resseq 263:278)C263 - 278
32X-RAY DIFFRACTION32chain 'C' and (resseq 279:290)C279 - 290
33X-RAY DIFFRACTION33chain 'D' and (resseq 159:183)D159 - 183
34X-RAY DIFFRACTION34chain 'D' and (resseq 184:194)D184 - 194
35X-RAY DIFFRACTION35chain 'D' and (resseq 195:223)D195 - 223
36X-RAY DIFFRACTION36chain 'D' and (resseq 224:233)D224 - 233
37X-RAY DIFFRACTION37chain 'D' and (resseq 234:262)D234 - 262
38X-RAY DIFFRACTION38chain 'D' and (resseq 263:272)D263 - 272
39X-RAY DIFFRACTION39chain 'D' and (resseq 273:290)D273 - 290
40X-RAY DIFFRACTION40chain 'E' and (resseq 156:183)E156 - 183
41X-RAY DIFFRACTION41chain 'E' and (resseq 184:194)E184 - 194
42X-RAY DIFFRACTION42chain 'E' and (resseq 195:203)E195 - 203
43X-RAY DIFFRACTION43chain 'E' and (resseq 204:213)E204 - 213
44X-RAY DIFFRACTION44chain 'E' and (resseq 214:222)E214 - 222
45X-RAY DIFFRACTION45chain 'E' and (resseq 223:230)E223 - 230
46X-RAY DIFFRACTION46chain 'E' and (resseq 231:262)E231 - 262
47X-RAY DIFFRACTION47chain 'E' and (resseq 263:272)E263 - 272
48X-RAY DIFFRACTION48chain 'E' and (resseq 273:289)E273 - 289
49X-RAY DIFFRACTION49chain 'F' and (resseq 159:223)F159 - 223
50X-RAY DIFFRACTION50chain 'F' and (resseq 224:233)F224 - 233
51X-RAY DIFFRACTION51chain 'F' and (resseq 234:256)F234 - 256
52X-RAY DIFFRACTION52chain 'F' and (resseq 257:272)F257 - 272
53X-RAY DIFFRACTION53chain 'F' and (resseq 273:291)F273 - 291
54X-RAY DIFFRACTION54chain 'G' and (resseq 160:183)G160 - 183
55X-RAY DIFFRACTION55chain 'G' and (resseq 184:194)G184 - 194
56X-RAY DIFFRACTION56chain 'G' and (resseq 195:222)G195 - 222
57X-RAY DIFFRACTION57chain 'G' and (resseq 223:234)G223 - 234
58X-RAY DIFFRACTION58chain 'G' and (resseq 235:262)G235 - 262
59X-RAY DIFFRACTION59chain 'G' and (resseq 263:271)G263 - 271
60X-RAY DIFFRACTION60chain 'G' and (resseq 272:278)G272 - 278
61X-RAY DIFFRACTION61chain 'G' and (resseq 279:288)G279 - 288

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