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Yorodumi- PDB-4g59: Crystal structure of the murine cytomegalovirus MHC-I homolog m15... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4g59 | ||||||
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Title | Crystal structure of the murine cytomegalovirus MHC-I homolog m152 with ligand RAE-1 gamma | ||||||
Components |
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Keywords | IMMUNE SYSTEM / MHC-I Fold / Immunoevasion / Stress induced ligand | ||||||
Function / homology | Function and homology information : / natural killer cell lectin-like receptor binding / cellular response to exogenous dsRNA / cellular response to lipopolysaccharide / membrane => GO:0016020 / defense response to bacterium / external side of plasma membrane / extracellular space Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) Murid herpesvirus 1 (Murine cytomegalovirus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.44 Å | ||||||
Authors | Wang, R. / Natarajan, K. / Margulies, D.H. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2012 Title: Structural basis of mouse cytomegalovirus m152/gp40 interaction with RAE1gamma reveals a paradigm for MHC/MHC interaction in immune evasion. Authors: Wang, R. / Natarajan, K. / Revilleza, M.J. / Boyd, L.F. / Zhi, L. / Zhao, H. / Robinson, H. / Margulies, D.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4g59.cif.gz | 186.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4g59.ent.gz | 147.1 KB | Display | PDB format |
PDBx/mmJSON format | 4g59.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/g5/4g59 ftp://data.pdbj.org/pub/pdb/validation_reports/g5/4g59 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 23201.994 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Raet1c / Production host: Escherichia coli (E. coli) / References: UniProt: O08604 #2: Protein | Mass: 36720.230 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Murid herpesvirus 1 (Murine cytomegalovirus) Gene: m152 / Production host: Drosophila (fruit flies) / References: UniProt: Q83156 #3: Sugar | ChemComp-NAG / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.71 Å3/Da / Density % sol: 54.69 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 15% PEG3350, 0.2M KCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 77 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X29A / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Mar 29, 2010 |
Radiation | Monochromator: Rosenbaum-Rock double crystal sagittal focusing monochromator Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.44→50 Å / Num. all: 47756 / Num. obs: 45215 / % possible obs: 94.7 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Biso Wilson estimate: 44.6 Å2 / Rmerge(I) obs: 0.07 |
Reflection shell | Resolution: 2.44→2.54 Å / Redundancy: 6.6 % / Rmerge(I) obs: 0.08 / Mean I/σ(I) obs: 2.8 / % possible all: 96.4 |
-Processing
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PBD entries 2O5N, 1JFM Resolution: 2.44→47.516 Å / SU ML: 0.28 / σ(F): 1 / Phase error: 27.35 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 51.8 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.44→47.516 Å
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Refine LS restraints |
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LS refinement shell |
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