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- PDB-4f5c: Crystal structure of the spike receptor binding domain of a porci... -

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Basic information

Entry
Database: PDB / ID: 4f5c
TitleCrystal structure of the spike receptor binding domain of a porcine respiratory coronavirus in complex with the pig aminopeptidase N ectodomain
Components
  • Aminopeptidase N
  • PRCV spike protein
KeywordsHYDROLASE/VIRAL PROTEIN / virus entry / cellular receptor / aminopeptidase N / glycosylation / virus membrane / metalloprotease / HYDROLASE-VIRAL PROTEIN complex
Function / homology
Function and homology information


membrane alanyl aminopeptidase / peptide catabolic process / metalloaminopeptidase activity / peptide binding / endocytosis involved in viral entry into host cell / virus receptor activity / angiogenesis / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / cell differentiation / receptor-mediated virion attachment to host cell ...membrane alanyl aminopeptidase / peptide catabolic process / metalloaminopeptidase activity / peptide binding / endocytosis involved in viral entry into host cell / virus receptor activity / angiogenesis / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / cell differentiation / receptor-mediated virion attachment to host cell / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion membrane / proteolysis / extracellular space / zinc ion binding / membrane / plasma membrane / cytoplasm
Similarity search - Function
Coronavirus S1 glycoprotein, central receptor binding domain (RBD) / Spike glycoprotein, Alphacoronavirus / Zincin-like fold - #20 / Immunoglobulin-like - #1910 / Spike glycoprotein S1, coronavirus / Coronavirus spike glycoprotein S1 / Aminopeptidase N-type / ERAP1-like C-terminal domain / ERAP1-like C-terminal domain / : ...Coronavirus S1 glycoprotein, central receptor binding domain (RBD) / Spike glycoprotein, Alphacoronavirus / Zincin-like fold - #20 / Immunoglobulin-like - #1910 / Spike glycoprotein S1, coronavirus / Coronavirus spike glycoprotein S1 / Aminopeptidase N-type / ERAP1-like C-terminal domain / ERAP1-like C-terminal domain / : / Zincin-like fold / tricorn interacting facor f3 domain / Peptidase M1, alanine aminopeptidase/leukotriene A4 hydrolase / Peptidase M1, membrane alanine aminopeptidase / Aminopeptidase N-like , N-terminal domain / Peptidase family M1 domain / Peptidase M1 N-terminal domain / Spike glycoprotein S2, coronavirus, C-terminal / Coronavirus spike glycoprotein S2, intravirion / Aminopeptidase N-like , N-terminal domain superfamliy / Neutral Protease Domain 2 / Neutral Protease; domain 2 / Peptidase M4/M1, CTD superfamily / Neutral zinc metallopeptidases, zinc-binding region signature. / Alpha Horseshoe / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal / Spike glycoprotein S2, coronavirus, heptad repeat 1 / Spike glycoprotein S2, coronavirus, heptad repeat 2 / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 1 (HR1) region profile. / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 2 (HR2) region profile. / Spike glycoprotein S2 superfamily, coronavirus / Spike glycoprotein S2, coronavirus / Coronavirus spike glycoprotein S2 / Immunoglobulin-like / Sandwich / Orthogonal Bundle / Mainly Beta / Mainly Alpha
Similarity search - Domain/homology
Aminopeptidase N / Spike glycoprotein
Similarity search - Component
Biological speciesSus scrofa (pig)
Porcine respiratory coronavirus
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.2 Å
AuthorsSantiago, C. / Reguera, J. / Gaurav, M. / Ordono, D. / Enjuanes, L. / Casasnovas, J.M.
CitationJournal: Plos Pathog. / Year: 2012
Title: Structural bases of coronavirus attachment to host aminopeptidase N and its inhibition by neutralizing antibodies.
Authors: Reguera, J. / Santiago, C. / Mudgal, G. / Ordono, D. / Enjuanes, L. / Casasnovas, J.M.
History
DepositionMay 13, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 22, 2012Provider: repository / Type: Initial release
Revision 2.0Jul 29, 2020Group: Atomic model / Data collection ...Atomic model / Data collection / Database references / Derived calculations / Structure summary
Category: atom_site / chem_comp ...atom_site / chem_comp / entity / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / pdbx_struct_conn_angle / struct_asym / struct_conn / struct_ref_seq_dif / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.type_symbol / _chem_comp.name / _chem_comp.type / _pdbx_entity_nonpoly.entity_id / _pdbx_entity_nonpoly.name / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.pdbx_role / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_ref_seq_dif.details
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1Oct 9, 2024Group: Data collection / Database references / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Aminopeptidase N
B: Aminopeptidase N
E: PRCV spike protein
F: PRCV spike protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)320,35429
Polymers314,5264
Non-polymers5,82825
Water724
1
A: Aminopeptidase N
E: PRCV spike protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)160,16814
Polymers157,2632
Non-polymers2,90512
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Aminopeptidase N
F: PRCV spike protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)160,18615
Polymers157,2632
Non-polymers2,92313
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)220.859, 87.938, 176.912
Angle α, β, γ (deg.)90.00, 90.54, 90.00
Int Tables number5
Space group name H-MC121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection details
111chain A and (resseq 99:140 or resseq 150:185 or resseq...
211chain B and (resseq 99:140 or resseq 150:185 or resseq...

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Components

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Protein , 2 types, 4 molecules ABEF

#1: Protein Aminopeptidase N / AP-N / pAPN / Alanyl aminopeptidase / Aminopeptidase M / AP-M / Microsomal aminopeptidase / gp130


Mass: 108896.977 Da / Num. of mol.: 2 / Fragment: UNP residues 36-963
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sus scrofa (pig) / Gene: ANPEP / Cell line (production host): CHO Lec 3.2.8.1 / Production host: Cricetulus griseus (Chinese hamster) / References: UniProt: P15145, membrane alanyl aminopeptidase
#2: Protein PRCV spike protein / S protein


Mass: 48365.875 Da / Num. of mol.: 2 / Fragment: receptor binding domain (UNP residues 17-445)
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Porcine respiratory coronavirus / Strain: HOL87 / Cell line (production host): CHO Lec 3.2.8.1 / Production host: Cricetulus griseus (Chinese hamster) / References: UniProt: Q84852

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Sugars , 2 types, 23 molecules

#3: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}}LINUCSPDB-CARE
#5: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 20
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 2 types, 6 molecules

#4: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Formula: H2O

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.73 Å3/Da / Density % sol: 54.96 %
Crystal growTemperature: 294 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: 20% PEG4000, 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.9791 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 6, 2011
RadiationMonochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9791 Å / Relative weight: 1
ReflectionResolution: 3.2→47.9 Å / Num. all: 56405 / Num. obs: 54019 / % possible obs: 96 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 3 % / Biso Wilson estimate: 88 Å2 / Rmerge(I) obs: 0.057 / Net I/σ(I): 13.2
Reflection shellResolution: 3.2→3.37 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.377 / Mean I/σ(I) obs: 2.1 / % possible all: 97.2

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Processing

Software
NameVersionClassification
DNAdata collection
PHASERphasing
PHENIX(phenix.refine: 1.7.1_743)refinement
XDSdata reduction
SCALAdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.2→24.912 Å / SU ML: 0.97 / σ(F): 1.34 / Phase error: 26.18 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2449 2732 5.07 %RANDOM
Rwork0.2013 ---
obs0.2034 51169 95.69 %-
all-53840 --
Solvent computationShrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 44.776 Å2 / ksol: 0.273 e/Å3
Displacement parameters
Baniso -1Baniso -2Baniso -3
1--10.4755 Å20 Å2-21.4329 Å2
2--3.3572 Å2-0 Å2
3---7.1184 Å2
Refinement stepCycle: LAST / Resolution: 3.2→24.912 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms16697 0 366 4 17067
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00317521
X-RAY DIFFRACTIONf_angle_d0.8823876
X-RAY DIFFRACTIONf_dihedral_angle_d19.1646372
X-RAY DIFFRACTIONf_chiral_restr0.0652707
X-RAY DIFFRACTIONf_plane_restr0.0033020
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDTypeRms dev position (Å)
11A6271X-RAY DIFFRACTIONPOSITIONAL
12B6271X-RAY DIFFRACTIONPOSITIONAL0.041
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.2-3.25510.3641360.32592572X-RAY DIFFRACTION97
3.2551-3.31410.32431370.30372586X-RAY DIFFRACTION97
3.3141-3.37770.36891390.29042564X-RAY DIFFRACTION97
3.3777-3.44650.361270.28152568X-RAY DIFFRACTION97
3.4465-3.52120.31761360.26792591X-RAY DIFFRACTION97
3.5212-3.60290.28621630.24072519X-RAY DIFFRACTION97
3.6029-3.69270.25541350.22432558X-RAY DIFFRACTION97
3.6927-3.79230.25331130.22282571X-RAY DIFFRACTION96
3.7923-3.90340.2541400.21132515X-RAY DIFFRACTION96
3.9034-4.02890.29151660.20972577X-RAY DIFFRACTION97
4.0289-4.17230.25991410.19842557X-RAY DIFFRACTION96
4.1723-4.33850.21691230.18032555X-RAY DIFFRACTION96
4.3385-4.53480.23491580.17042528X-RAY DIFFRACTION96
4.5348-4.77230.20961430.16322540X-RAY DIFFRACTION95
4.7723-5.0690.19041270.15872553X-RAY DIFFRACTION95
5.069-5.45660.2051230.15942569X-RAY DIFFRACTION95
5.4566-5.99870.23091210.18862550X-RAY DIFFRACTION95
5.9987-6.85090.26011460.2122534X-RAY DIFFRACTION94
6.8509-8.57260.22581300.18262537X-RAY DIFFRACTION93
8.5726-24.91240.1981280.19192564X-RAY DIFFRACTION92
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.3155-0.2861-0.63541.177-0.00571.67480.34490.7464-0.3957-0.6873-0.50140.40010.5853-0.16560.25980.99270.2111-0.14490.981-0.3730.78232.4084-54.6425-87.1212
23.2056-4.3773.89457.138-5.91845.0298-0.37940.50070.8223-0.22050.0752-0.311-0.56060.62680.25811.7128-0.3579-0.31211.6851-0.1761.167722.7127-68.9678-95.054
30.9197-0.46350.1740.7169-0.33041.0145-0.28340.29670.1052-0.3374-0.6412-0.26110.36970.00720.18970.87560.0201-0.0780.6362-0.35190.917133.8206-55.0567-86.7265
44.763-0.60373.05991.4952-0.64533.45270.20210.1127-0.0334-0.44030.0502-0.1498-0.302-0.13180.13970.56070.0555-0.0680.4086-0.23910.533430.5969-49.865-74.7175
51.0706-0.2676-0.26572.06960.41121.68390.1436-0.1011-0.50250.08970.2385-0.01290.3935-0.0567-0.25450.52180.0228-0.19120.3998-0.06230.450823.2931-49.6137-61.5001
61.5328-0.15060.89311.6460.18182.11110.19460.1887-0.1534-0.1228-0.04080.14660.2489-0.1652-0.17090.18560.0128-0.03990.3739-0.00360.298611.2934-28.1555-75.7457
72.49090.33561.69581.1217-0.26481.0737-0.1494-0.12140.3216-0.06840.05270.1857-0.1678-0.37750.06230.29690.09670.0850.38-0.06860.369114.0244-9.4169-60.8095
82.20350.31580.69821.5929-1.21642.15070.01270.2052-0.0631-0.2866-0.1407-0.2010.23750.18950.0750.35720.00930.0820.2733-0.05140.31542.0419-15.7824-56.6205
91.34330.0726-0.40561.11310.31661.1659-0.35620.25120.2189-1.0759-0.2945-0.2401-1.11070.2590.37581.5885-0.2252-0.3250.69470.53210.953679.3782-40.2721-55.9404
100.1076-0.0487-0.10480.79791.32382.0596-0.69020.04220.5999-0.8343-0.1689-0.6085-0.85730.7410.27252.1892-0.32790.01441.22340.38320.79387.1957-35.9586-61.5768
112.0253-0.68940.90441.21070.73842.2151-0.43010.72760.0519-1.078-0.3320.4025-0.7471-0.45050.41781.2268-0.1896-0.40590.43050.4860.73870.5243-45.2225-49.4972
120.5880.4340.15692.402-0.06030.993-0.25510.00680.4264-0.3572-0.08170.4926-0.3031-0.28890.25960.63070.0496-0.19590.45790.11190.744866.4547-50.2294-33.5724
131.0383-0.09760.33922.17910.61851.34860.01510.14670.2552-0.2063-0.1686-0.5759-0.26290.24510.13040.411-0.04790.02450.3960.12680.536386.6209-70.2005-37.5217
142.69-0.37170.48352.4734-0.52424.11810.33560.2715-0.3899-0.06950.1412-0.79270.00130.3269-0.02290.37540.05030.03390.6427-0.0070.700193.7295-87.8451-34.4266
151.958-0.18980.02710.8710.27381.62140.0117-0.1380.01910.1032-0.18490.0435-0.0694-0.06050.10850.3970.01080.04950.2640.00290.368363.3781-89.3663-31.6694
162.6363-0.7885-0.02662.39650.54571.87740.05370.52090.5445-0.3122-0.1995-0.1532-0.30460.04960.19530.39260.01680.03420.35180.0230.537955.3208-83.3987-54.2525
173.27172.2208-3.4944.9198-3.84276.03850.103-0.2298-0.14130.44950.11810.21370.13810.5612-0.15990.49010.12090.14550.6140.08130.442215.0872-34.3986-19.1057
182.70084.29482.49116.8434.02562.414-0.3237-0.07450.1942-0.2142-0.0353-0.56810.0080.8536-0.32760.9605-0.0530.26180.913-0.10960.7755.4456-45.8417-21.9192
194.44620.1151-3.37711.3896-0.64585.2364-0.7789-0.6446-0.5860.0532-0.1373-0.3403-0.0551.53320.55930.5564-0.00920.01580.67370.19270.626819.0125-35.2774-20.0717
200.6858-0.1947-1.15030.36030.44014.7908-0.4375-0.74090.19020.8812-0.07310.0934-0.19060.21910.42130.7956-0.07560.06160.80280.09720.84020.4302-32.0894-16.0051
215.92912.1053-1.763.35150.10522.78120.0451-0.2910.57210.2926-0.06560.2284-0.58010.1745-0.22080.6935-0.11330.09090.63320.17930.45638.7234-28.7616-19.7698
220.93370.60832.51784.53991.4656.7938-0.0048-0.59420.67471.02670.16230.1776-0.57660.4924-0.13051.1173-0.18170.04250.9146-0.04620.8571-0.6593-37.5054-4.9909
230.0986-0.0784-0.10190.04960.07340.10150.14210.0439-0.6754-0.44310.3353-0.73911.20440.8907-0.46041.12180.22470.19191.3913-0.48181.085632.5408-68.043321.3101
240.95490.303-0.37960.78380.06970.6978-0.3783-0.16810.55610.2786-0.2058-0.0299-0.52490.19590.27980.87080.10740.13391.0613-0.23580.960643.0994-73.8732-4.6634
250.42280.3285-0.59560.6161-1.04135.21710.1742-0.09040.32430.14440.2960.19410.2102-0.2367-0.10620.88810.08710.09111.0525-0.25760.943238.215-73.8516-4.9273
263.70052.20341.33742.58190.55210.55040.18610.2682-0.41330.02140.2299-0.28730.8061.1364-0.2281.19120.305-0.05591.2985-0.14180.863646.7848-79.125911.8733
276.5892.60864.51366.76521.46676.5128-0.3592-0.5288-0.8571-0.06740.127-0.55140.76180.08410.01190.83370.03920.26541.1125-0.19890.620642.3644-82.3122-4.1127
283.6447-1.1185-3.19352.5156-0.22394.5144-0.336-0.2318-0.58820.8634-0.02560.15520.34250.46960.24631.49740.0639-0.00371.4701-0.42311.36844.2879-77.391219.6245
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(chain A and resid 61:133)
2X-RAY DIFFRACTION2(chain A and resid 134:142)
3X-RAY DIFFRACTION3(chain A and resid 143:194)
4X-RAY DIFFRACTION4(chain A and resid 195:229)
5X-RAY DIFFRACTION5(chain A and resid 230:386)
6X-RAY DIFFRACTION6(chain A and resid 387:564)
7X-RAY DIFFRACTION7(chain A and resid 565:756)
8X-RAY DIFFRACTION8(chain A and resid 757:965)
9X-RAY DIFFRACTION9(chain B and resid 60:138)
10X-RAY DIFFRACTION10(chain B and resid 139:167)
11X-RAY DIFFRACTION11(chain B and resid 168:228)
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