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- PDB-4aez: Crystal Structure of Mitotic Checkpoint Complex -

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Basic information

Entry
Database: PDB / ID: 4aez
TitleCrystal Structure of Mitotic Checkpoint Complex
Components
  • MITOTIC SPINDLE CHECKPOINT COMPONENT MAD2
  • MITOTIC SPINDLE CHECKPOINT COMPONENT MAD3
  • WD REPEAT-CONTAINING PROTEIN SLP1
KeywordsCELL CYCLE / KEN-BOX / D-BOX / APC/C
Function / homology
Function and homology information


meiosis I spindle assembly checkpoint signaling / metaphase/anaphase transition of meiosis II / Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components / Inactivation of APC/C via direct inhibition of the APC/C complex / metaphase/anaphase transition of meiosis I / negative regulation of mitotic metaphase/anaphase transition / mitotic checkpoint complex / positive regulation of anaphase-promoting complex-dependent catabolic process / Antigen processing: Ubiquitination & Proteasome degradation / meiotic spindle assembly checkpoint signaling ...meiosis I spindle assembly checkpoint signaling / metaphase/anaphase transition of meiosis II / Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components / Inactivation of APC/C via direct inhibition of the APC/C complex / metaphase/anaphase transition of meiosis I / negative regulation of mitotic metaphase/anaphase transition / mitotic checkpoint complex / positive regulation of anaphase-promoting complex-dependent catabolic process / Antigen processing: Ubiquitination & Proteasome degradation / meiotic spindle assembly checkpoint signaling / mitotic spindle pole body / anaphase-promoting complex / anaphase-promoting complex-dependent catabolic process / mitotic spindle microtubule / anaphase-promoting complex binding / positive regulation of mitotic metaphase/anaphase transition / ubiquitin ligase activator activity / cell division site / mitotic spindle assembly checkpoint signaling / ubiquitin ligase inhibitor activity / nuclear periphery / kinetochore / mitotic spindle / cell division / chromatin / nucleoplasm / nucleus / cytosol / cytoplasm
Similarity search - Function
Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat - #430 / Mad3/Bub1 homology region 1 / Mitotic spindle checkpoint protein Bub1/Mad3 / Mad3/BUB1 homology region 1 / BUB1 N-terminal domain profile. / Mad3/BUB1 hoMad3/BUB1 homology region 1 / : / CDC20/Fizzy WD40 domain / Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A / HORMA domain ...Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat - #430 / Mad3/Bub1 homology region 1 / Mitotic spindle checkpoint protein Bub1/Mad3 / Mad3/BUB1 homology region 1 / BUB1 N-terminal domain profile. / Mad3/BUB1 hoMad3/BUB1 homology region 1 / : / CDC20/Fizzy WD40 domain / Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A / HORMA domain / The WD repeat Cdc20/Fizzy family / Mad2-like / HORMA domain / HORMA domain / HORMA domain profile. / HORMA domain superfamily / YVTN repeat-like/Quinoprotein amine dehydrogenase / 7 Propeller / Methylamine Dehydrogenase; Chain H / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Alpha Horseshoe / Tetratricopeptide-like helical domain superfamily / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / WD domain, G-beta repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / 2-Layer Sandwich / Mainly Beta / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
Mitotic spindle checkpoint component mad2 / Mitotic spindle checkpoint component mad3 / WD repeat-containing protein slp1
Similarity search - Component
Biological speciesSCHIZOSACCHAROMYCES POMBE (fission yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å
AuthorsKulkarni, K.A. / Chao, W.C.H. / Zhang, Z. / Barford, D.
CitationJournal: Nature / Year: 2012
Title: Structure of the Mitotic Checkpoint Complex
Authors: Chao, W.C.H. / Kulkarni, K.A. / Zhang, Z. / Kong, E.H. / Barford, D.
History
DepositionJan 13, 2012Deposition site: PDBE / Processing site: PDBE
Revision 1.0Mar 21, 2012Provider: repository / Type: Initial release
Revision 1.1Apr 25, 2012Group: Other
Revision 1.2Apr 3, 2019Group: Data collection / Other / Source and taxonomy
Category: entity_src_gen / pdbx_database_proc / pdbx_database_status
Item: _entity_src_gen.pdbx_host_org_cell_line / _pdbx_database_status.recvd_author_approval
Revision 1.3May 29, 2019Group: Data collection / Experimental preparation / Category: exptl_crystal_grow / struct_biol / Item: _exptl_crystal_grow.method
Revision 1.4Dec 20, 2023Group: Data collection / Database references ...Data collection / Database references / Other / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_sf

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: WD REPEAT-CONTAINING PROTEIN SLP1
B: MITOTIC SPINDLE CHECKPOINT COMPONENT MAD2
C: MITOTIC SPINDLE CHECKPOINT COMPONENT MAD3
D: WD REPEAT-CONTAINING PROTEIN SLP1
E: MITOTIC SPINDLE CHECKPOINT COMPONENT MAD2
F: MITOTIC SPINDLE CHECKPOINT COMPONENT MAD3
G: WD REPEAT-CONTAINING PROTEIN SLP1
H: MITOTIC SPINDLE CHECKPOINT COMPONENT MAD2
I: MITOTIC SPINDLE CHECKPOINT COMPONENT MAD3


Theoretical massNumber of molelcules
Total (without water)281,5249
Polymers281,5249
Non-polymers00
Water5,729318
1
A: WD REPEAT-CONTAINING PROTEIN SLP1
B: MITOTIC SPINDLE CHECKPOINT COMPONENT MAD2
C: MITOTIC SPINDLE CHECKPOINT COMPONENT MAD3


Theoretical massNumber of molelcules
Total (without water)93,8413
Polymers93,8413
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6610 Å2
ΔGint-20.5 kcal/mol
Surface area32540 Å2
MethodPISA
2
D: WD REPEAT-CONTAINING PROTEIN SLP1
E: MITOTIC SPINDLE CHECKPOINT COMPONENT MAD2
F: MITOTIC SPINDLE CHECKPOINT COMPONENT MAD3


Theoretical massNumber of molelcules
Total (without water)93,8413
Polymers93,8413
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5620 Å2
ΔGint-24.3 kcal/mol
Surface area29730 Å2
MethodPISA
3
G: WD REPEAT-CONTAINING PROTEIN SLP1
H: MITOTIC SPINDLE CHECKPOINT COMPONENT MAD2
I: MITOTIC SPINDLE CHECKPOINT COMPONENT MAD3


Theoretical massNumber of molelcules
Total (without water)93,8413
Polymers93,8413
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5790 Å2
ΔGint-25.2 kcal/mol
Surface area30540 Å2
MethodPISA
Unit cell
Length a, b, c (Å)70.810, 286.900, 72.010
Angle α, β, γ (deg.)90.00, 119.04, 90.00
Int Tables number4
Space group name H-MP1211

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Components

#1: Protein WD REPEAT-CONTAINING PROTEIN SLP1 / CDC20


Mass: 43980.910 Da / Num. of mol.: 3 / Fragment: RESIDUES 88-488
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) SCHIZOSACCHAROMYCES POMBE (fission yeast)
Plasmid: PFBDM / Cell line (production host): High Five / Production host: TRICHOPLUSIA NI (cabbage looper) / References: UniProt: P78972
#2: Protein MITOTIC SPINDLE CHECKPOINT COMPONENT MAD2 / MAD2


Mass: 23482.852 Da / Num. of mol.: 3 / Mutation: YES
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) SCHIZOSACCHAROMYCES POMBE (fission yeast)
Plasmid: PFBDM / Cell line (production host): High Five / Production host: TRICHOPLUSIA NI (cabbage looper) / References: UniProt: O14417
#3: Protein MITOTIC SPINDLE CHECKPOINT COMPONENT MAD3 / MAD3


Mass: 26377.539 Da / Num. of mol.: 3 / Fragment: RESIDUES 1-233
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) SCHIZOSACCHAROMYCES POMBE (fission yeast)
Plasmid: PFBDM / Cell line (production host): High Five / Production host: TRICHOPLUSIA NI (cabbage looper) / References: UniProt: O59767
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 318 / Source method: isolated from a natural source / Formula: H2O
Compound detailsENGINEERED RESIDUE IN CHAIN B, LEU 12 TO ALA ENGINEERED RESIDUE IN CHAIN B, ARG 133 TO ALA ...ENGINEERED RESIDUE IN CHAIN B, LEU 12 TO ALA ENGINEERED RESIDUE IN CHAIN B, ARG 133 TO ALA ENGINEERED RESIDUE IN CHAIN E, LEU 12 TO ALA ENGINEERED RESIDUE IN CHAIN E, ARG 133 TO ALA ENGINEERED RESIDUE IN CHAIN H, LEU 12 TO ALA ENGINEERED RESIDUE IN CHAIN H, ARG 133 TO ALA

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.28 Å3/Da / Density % sol: 46.04 % / Description: NONE
Crystal growMethod: vapor diffusion, hanging drop / pH: 8.8
Details: CRYSTALS WERE OBTAINED IN A HANGING-DROP FASHION BY PRE-INCUBATING 1:1 V/V OF 4.5 MG/ML OF PROTEIN WITH THE CRYSTALLIZATION SOLUTION, 100 MM TRIS-HCL PH 8.8, 21% PEG 3350, 30% ETHYLENE ...Details: CRYSTALS WERE OBTAINED IN A HANGING-DROP FASHION BY PRE-INCUBATING 1:1 V/V OF 4.5 MG/ML OF PROTEIN WITH THE CRYSTALLIZATION SOLUTION, 100 MM TRIS-HCL PH 8.8, 21% PEG 3350, 30% ETHYLENE GLYCOL, 5 MM DTT, AND 5 MM EDTA AT 20 OC FOR 24 HOURS, FOLLOWED BY STREAK SEEDING.

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I02 / Wavelength: 0.9795
DetectorType: ADSC CCD / Detector: CCD / Date: Feb 14, 2011
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9795 Å / Relative weight: 1
ReflectionResolution: 2.3→62.96 Å / Num. obs: 110660 / % possible obs: 99.9 % / Observed criterion σ(I): 1.5 / Redundancy: 5.5 % / Biso Wilson estimate: 41.76 Å2 / Rmerge(I) obs: 0.06 / Net I/σ(I): 16.4
Reflection shellResolution: 2.3→2.42 Å / Redundancy: 5.6 % / Rmerge(I) obs: 0.59 / Mean I/σ(I) obs: 3.1 / % possible all: 100

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Processing

Software
NameVersionClassification
PHENIX(PHENIX.REFINE)refinement
XDSdata reduction
SCALAdata scaling
AMoREphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRIES 2VFX, 2WVI, 3EMH

2vfx

2wvi

3emh


Resolution: 2.3→62.957 Å / SU ML: 0.83 / σ(F): 1.35 / Phase error: 25.6 / Stereochemistry target values: ML
Details: DISORDERED REGIONS HAVE BEEN OMITTED IN THE REFINEMENT.
RfactorNum. reflection% reflection
Rfree0.2671 1973 1.8 %
Rwork0.2207 --
obs0.2215 110586 99.84 %
Solvent computationShrinkage radii: 0.95 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 48.44 Å2 / ksol: 0.347 e/Å3
Displacement parametersBiso mean: 60.41 Å2
Baniso -1Baniso -2Baniso -3
1-3.6148 Å20 Å21.766 Å2
2---2.3924 Å20 Å2
3----1.2223 Å2
Refinement stepCycle: LAST / Resolution: 2.3→62.957 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms16578 0 0 318 16896
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00616958
X-RAY DIFFRACTIONf_angle_d0.95923042
X-RAY DIFFRACTIONf_dihedral_angle_d14.9225998
X-RAY DIFFRACTIONf_chiral_restr0.072557
X-RAY DIFFRACTIONf_plane_restr0.0042957
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.3-2.35750.37041320.30987767X-RAY DIFFRACTION100
2.3575-2.42130.36521450.29847785X-RAY DIFFRACTION100
2.4213-2.49250.33391350.27447740X-RAY DIFFRACTION100
2.4925-2.5730.34911270.25377791X-RAY DIFFRACTION100
2.573-2.6650.28691340.22967747X-RAY DIFFRACTION100
2.665-2.77170.31721480.22327742X-RAY DIFFRACTION100
2.7717-2.89780.28461560.22577744X-RAY DIFFRACTION100
2.8978-3.05060.2511550.2177728X-RAY DIFFRACTION100
3.0506-3.24170.25141400.21277770X-RAY DIFFRACTION100
3.2417-3.4920.22581420.21367759X-RAY DIFFRACTION100
3.492-3.84330.25581490.20317756X-RAY DIFFRACTION100
3.8433-4.39940.23921460.19377752X-RAY DIFFRACTION100
4.3994-5.54220.25121270.1967797X-RAY DIFFRACTION100
5.5422-62.98050.2711370.24257735X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
15.808-3.8174-2.07474.98412.22231.03960.18910.1326-0.1964-0.0276-0.2065-0.31970.1542-0.3520.00910.3723-0.0475-0.13470.497-0.00310.26574.8867-14.308850.7481
21.89610.60050.16283.1097-0.72353.1965-0.13170.09870.4298-0.3520.10090.0749-0.3266-0.3226-0.05370.18310.0583-0.03710.22110.00880.2288-21.199414.693110.086
33.4333-0.2040.67144.11950.0821.3566-0.06570.17110.2086-0.13340.05580.3771-0.079-0.57180.01160.09410.042-0.00020.372-0.02610.1943-34.54334.292214.636
41.08340.195-0.15432.22520.04190.9097-0.2040.2257-0.1408-0.41840.1608-0.35880.0536-0.01830.04610.15460.01070.06060.2087-0.07650.1715-14.0114-3.185710.534
54.7561-1.3652-1.73226.26455.45694.81970.18931.8033-0.448-1.8654-0.462-0.9566-0.02180.12530.28450.5930.09940.2070.52230.03460.3982-8.95710.2114-1.6864
62.71440.32930.07174.9662-0.40225.01180.09070.2286-0.4769-0.29-0.1227-0.32060.7027-0.069-0.03630.23530.0004-0.02890.2209-0.03720.2374-1.522-19.793439.3606
73.88190.6248-1.51516.5639-1.97846.95620.0224-0.25010.01470.1706-0.0861-0.26280.1968-0.07460.02590.1506-0.0007-0.13410.2564-0.00710.1786-4.1127-13.889951.2143
83.47922.3654-0.07085.96640.09081.74190.0804-0.20840.03760.0557-0.14560.40630.4174-0.38660.03330.2252-0.0938-0.0810.3403-0.04720.2122-14.9591-14.745938.5132
91.05520.3237-0.06754.1016-2.12244.9178-0.0955-0.29190.0340.7167-0.2938-0.6993-0.51940.28910.12050.29470-0.15910.41680.01480.2775-0.9262-11.346755.8788
104.70373.5589-0.71663.1801-1.13854.01690.1378-0.34340.0150.2782-0.22250.02330.0345-0.56750.12180.1806-0.0816-0.10020.3464-0.00740.1338-13.2-7.683845.2116
116.44091.5579-1.09985.3491-0.00037.25650.1745-0.4160.11470.3862-0.2295-0.0007-0.2002-0.3651-0.05680.15170.0242-0.0640.139-0.03950.1707-12.04933.142734.8431
120.68740.5964-0.80632.61080.74521.9624-0.0238-0.27310.72450.0790.03360.0093-0.32170.06730.01420.31050.07-0.07830.2188-0.12440.3817-2.475420.798134.7597
134.8213-0.4782-0.67877.9524-1.99225.9882-0.1356-0.22650.483-0.3061-0.2621-0.9652-0.080.38140.39680.13320.05190.0240.3173-0.04650.462216.497213.042528.4425
142.4679-1.6517-1.80022.25742.30124.8764-0.501-0.3565-0.18580.39520.233-0.06910.91790.70090.27760.28520.13950.16820.32770.12540.482922.80840.353130.3493
156.08953.60051.57595.5172-0.61868.97160.1518-0.3090.868-0.1853-0.0569-0.6239-1.0481-0.1448-0.22670.5762-0.10440.13010.2635-0.12740.428244.5886-20.959726.1137
161.83281.23830.58772.2795-0.26660.4911-0.4597-0.0737-0.43790.37290.243-0.40770.2906-0.45180.36080.9269-0.06880.09860.64280.31510.7836-46.043-58.787350.5467
173.02760.862-1.21223.08660.30783.51750.03610.22170.2122-0.32470.0504-0.2142-0.3601-0.2414-0.00950.21360.00110.03470.1103-0.02250.1301-34.1286-31.835717.4844
180.7890.0970.56142.16510.28090.9446-0.27060.6224-0.1654-0.7339-0.04540.12650.2827-0.3475-0.02720.4602-0.2285-0.00080.5917-0.19210.1722-43.9242-41.09898.0417
190.3463-0.188-0.5631.0620.41590.9377-0.39020.1917-0.6328-0.1752-0.0226-0.34240.74740.0437-0.23690.38730.09370.3525-0.2953-0.2910.3044-32.7439-54.035223.7368
202.44590.2897-1.79752.73690.91297.4094-0.1437-0.3916-0.2423-0.0429-0.0129-0.75760.3220.7649-0.07320.1371-0.03480.02730.186-0.0050.2954-25.1547-37.158126.5829
210.6749-0.7436-0.63273.6947-1.00722.1972-0.20.0149-0.92910.0065-0.11930.06550.8199-0.31090.04940.8326-0.2230.12960.44390.05730.6834-53.167-67.697343.4195
225.07511.1374-4.01126.6194-3.03825.20650.1183-0.9787-0.22670.7332-0.1751-0.0532-0.33520.73440.09110.9262-0.31630.10471.05990.3710.6925-56.0954-54.427454.9467
231.83660.6226-0.55163.0022-0.03170.2360.2794-0.1374-0.95490.1877-0.20380.20860.9771-0.7617-0.11130.5652-0.2156-0.01880.422-0.00310.5473-55.6575-56.329337.4312
246.02361.2023-0.79064.6301-1.64015.20030.72560.142-0.89870.9387-0.2076-0.15420.0975-0.6543-0.53970.7847-0.2759-0.02361.0380.32750.8777-63.3293-60.986854.2173
254.81461.92480.27526.61682.01081.54760.3896-0.2833-0.61530.1741-0.24060.5190.646-0.736-0.06980.5027-0.30.00620.4743-0.04310.4776-60.3969-52.768939.9932
263.53830.43040.90984.03470.85042.9482-0.33240.2364-0.284-0.121-0.05220.3030.3011-0.65660.10220.2395-0.10250.0810.2692-0.06990.2323-51.2551-41.842837.0276
273.0009-0.16510.25214.01091.44954.25070.0063-0.24080.18850.3278-0.0444-0.0023-0.1152-0.0299-0.00460.2391-0.00070.05340.1385-0.03270.1631-44.1861-25.671647.9995
282.71050.77830.310.5305-0.70832.44250.1273-0.5835-0.6430.8111-0.2419-0.66070.11190.49190.03580.8065-0.0577-0.40250.51110.21740.6106-30.8272-39.119462.6143
290.5130.33490.28350.27460.29190.360.0017-0.147-0.1463-0.00320.07640.1555-0.0802-0.1712-0.13341.5834-0.4512-0.59541.01430.52271.1244-40.0993-45.804769.0912
303.96971.34691.54078.5687-1.69573.8077-0.64310.04730.1278-0.42920.2447-0.086-0.4965-0.37960.49190.8949-0.03910.14920.7336-0.060.6541-18.7108-69.144981.5482
312.8104-2.1451.06662.7388-0.48233.30040.63781.24380.1598-0.6603-0.656-0.23620.47941.29980.15840.6303-0.08420.21880.70390.14390.6165-27.500739.674855.7684
321.9359-0.67161.04172.1221-0.3671.918-0.32770.06480.2351-0.39440.19120.4306-0.9577-0.16260.17510.80880.07270.04580.33460.11760.7405-56.397166.371652.536
333.4314-0.68461.32112.3630.21921.7831-0.4703-0.41890.04910.13660.22320.5178-0.931-0.55940.25530.88460.30120.1820.4290.08990.7373-59.998263.654865.1232
340.6235-0.07410.21021.8574-1.07670.7742-0.009-0.2942-0.45980.13250.25970.9394-0.0432-0.37460.08960.5253-0.05220.24550.14620.11571.0222-55.655745.004458.4198
351.9996-0.42980.57284.28240.53114.0347-0.36260.1744-0.5185-1.40850.07090.8665-0.1536-0.13790.10320.82-0.0179-0.02320.344-0.110.8724-53.749156.073343.5715
361.38720.26972.452.9828-1.30225.50090.01070.001-0.0293-0.1403-0.2715-0.11010.47030.40990.0140.40630.00850.20450.54430.04850.6298-29.495328.677767.4161
373.83830.545-1.13691.56720.15683.94750.2030.1393-0.0058-0.1661-0.0895-0.2992-0.31741.1624-0.16270.2617-0.06150.21780.58430.09910.8921-20.492839.960166.3056
380.78380.67471.5555.8069-2.2645.5767-0.3096-0.0531-0.4815-0.4838-0.0254-0.04640.6921-0.03640.40740.31840.00440.13420.26350.050.5716-37.311939.478870.1128
394.0479-0.64934.77058.8907-4.83387.5058-0.24310.2402-0.6344-1.8854-0.3115-0.85871.32651.03490.53571.88660.3620.38450.84890.12091.0572-18.746638.118252.036
401.93311.3529-0.96023.7235-0.91294.9776-0.075-0.05820.0143-0.0726-0.2273-0.98650.07161.22940.05570.4212-0.07870.02030.68270.16840.8253-19.982338.195371.1476
416.49057.3809-0.00428.722-1.26294.8191-0.3156-0.0489-1.0242-0.8089-0.0061-0.57160.34520.90030.32511.189-0.10250.34870.54810.12030.6233-29.122451.197270.5881
420.8869-0.0294-0.30861.38580.78070.5911-0.06990.15610.56-0.028-0.1304-0.0724-0.47060.2691-0.67670.7835-0.35070.23330.30960.2550.846-29.116865.799554.647
432.5066-0.4060.01510.4243-0.17790.2977-0.26140.56550.0655-0.75820.1726-0.8129-0.12760.65840.05350.8209-0.24850.45381.00920.0650.8243-20.778862.976339.3723
443.705-1.83441.43452.5558-0.38313.00140.29230.3511-0.5595-0.4748-0.1426-0.2051-0.70440.6555-0.1730.87490.06050.550.7014-0.03350.8997-18.976847.233933.4591
457.2025-0.4473-1.13758.1204-1.37752.61410.46890.39170.7301-0.2939-0.0520.0188-1.0081-0.5159-0.38560.53620.1096-0.00040.26250.14850.52-13.106724.86475.5792
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(CHAIN A AND RESID 125:144)
2X-RAY DIFFRACTION2(CHAIN A AND RESID 162:234)
3X-RAY DIFFRACTION3(CHAIN A AND RESID 235:302)
4X-RAY DIFFRACTION4(CHAIN A AND RESID 303:461)
5X-RAY DIFFRACTION5(CHAIN A AND RESID 462:467)
6X-RAY DIFFRACTION6(CHAIN B AND RESID 13:64)
7X-RAY DIFFRACTION7(CHAIN B AND RESID 65:103)
8X-RAY DIFFRACTION8(CHAIN B AND RESID 104:145)
9X-RAY DIFFRACTION9(CHAIN B AND RESID 146:176)
10X-RAY DIFFRACTION10(CHAIN B AND RESID 177:201)
11X-RAY DIFFRACTION11(CHAIN C AND RESID 9:37)
12X-RAY DIFFRACTION12(CHAIN C AND RESID 38:129)
13X-RAY DIFFRACTION13(CHAIN C AND RESID 130:162)
14X-RAY DIFFRACTION14(CHAIN C AND RESID 163:209)
15X-RAY DIFFRACTION15(CHAIN C AND RESID 210:223)
16X-RAY DIFFRACTION16(CHAIN D AND RESID 129:166)
17X-RAY DIFFRACTION17(CHAIN D AND RESID 167:241)
18X-RAY DIFFRACTION18(CHAIN D AND RESID 242:302)
19X-RAY DIFFRACTION19(CHAIN D AND RESID 303:430)
20X-RAY DIFFRACTION20(CHAIN D AND RESID 431:467)
21X-RAY DIFFRACTION21(CHAIN E AND RESID 14:81)
22X-RAY DIFFRACTION22(CHAIN E AND RESID 82:96)
23X-RAY DIFFRACTION23(CHAIN E AND RESID 97:145)
24X-RAY DIFFRACTION24(CHAIN E AND RESID 146:177)
25X-RAY DIFFRACTION25(CHAIN E AND RESID 178:202)
26X-RAY DIFFRACTION26(CHAIN F AND RESID 10:41)
27X-RAY DIFFRACTION27(CHAIN F AND RESID 42:129)
28X-RAY DIFFRACTION28(CHAIN F AND RESID 130:178)
29X-RAY DIFFRACTION29(CHAIN F AND RESID 179:194)
30X-RAY DIFFRACTION30(CHAIN F AND RESID 195:222)
31X-RAY DIFFRACTION31(CHAIN G AND RESID 126:170)
32X-RAY DIFFRACTION32(CHAIN G AND RESID 171:214)
33X-RAY DIFFRACTION33(CHAIN G AND RESID 215:281)
34X-RAY DIFFRACTION34(CHAIN G AND RESID 282:424)
35X-RAY DIFFRACTION35(CHAIN G AND RESID 425:467)
36X-RAY DIFFRACTION36(CHAIN H AND RESID 6:84)
37X-RAY DIFFRACTION37(CHAIN H AND RESID 85:106)
38X-RAY DIFFRACTION38(CHAIN H AND RESID 115:145)
39X-RAY DIFFRACTION39(CHAIN H AND RESID 146:151)
40X-RAY DIFFRACTION40(CHAIN H AND RESID 152:201)
41X-RAY DIFFRACTION41(CHAIN I AND RESID 10:14)
42X-RAY DIFFRACTION42(CHAIN I AND RESID 15:104)
43X-RAY DIFFRACTION43(CHAIN I AND RESID 105:134)
44X-RAY DIFFRACTION44(CHAIN I AND RESID 135:198)
45X-RAY DIFFRACTION45(CHAIN I AND RESID 214:222)

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