- PDB-4a4m: Crystal structure of the light-activated constitutively active N2... -
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基本情報
登録情報
データベース: PDB / ID: 4a4m
タイトル
Crystal structure of the light-activated constitutively active N2C, M257Y,D282C rhodopsin mutant in complex with a peptide resembling the C-terminus of the Galpha-protein subunit (GaCT)
要素
GUANINE NUCLEOTIDE-BINDING PROTEIN G(T) SUBUNIT ALPHA-3
RHODOPSIN
キーワード
SIGNALING PROTEIN / G-PROTEIN / G-PROTEIN-COUPLED RECEPTORS / SIGNAL TANSDUCTION / VISUAL SYSTEM / METARHODOPSIN-II
機能・相同性
機能・相同性情報
Adenylate cyclase inhibitory pathway / detection of light stimulus involved in visual perception / negative regulation of cyclic-nucleotide phosphodiesterase activity / Opsins / VxPx cargo-targeting to cilium / opsin binding / rod bipolar cell differentiation / rod photoreceptor outer segment / sperm head plasma membrane / sensory perception of umami taste ...Adenylate cyclase inhibitory pathway / detection of light stimulus involved in visual perception / negative regulation of cyclic-nucleotide phosphodiesterase activity / Opsins / VxPx cargo-targeting to cilium / opsin binding / rod bipolar cell differentiation / rod photoreceptor outer segment / sperm head plasma membrane / sensory perception of umami taste / absorption of visible light / Sensory perception of sweet, bitter, and umami (glutamate) taste / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / The canonical retinoid cycle in rods (twilight vision) / sensory perception of sweet taste / : / photoreceptor inner segment membrane / podosome assembly / G protein-coupled opsin signaling pathway / 11-cis retinal binding / G protein-coupled photoreceptor activity / detection of chemical stimulus involved in sensory perception of bitter taste / cellular response to light stimulus / G protein-coupled receptor complex / Inactivation, recovery and regulation of the phototransduction cascade / thermotaxis / Activation of the phototransduction cascade / phototransduction, visible light / outer membrane / detection of temperature stimulus involved in thermoception / photoreceptor cell maintenance / arrestin family protein binding / ADP signalling through P2Y purinoceptor 12 / Extra-nuclear estrogen signaling / photoreceptor outer segment membrane / G alpha (i) signalling events / phototransduction / photoreceptor outer segment / G-protein alpha-subunit binding / response to light stimulus / acyl binding / sperm midpiece / visual perception / photoreceptor inner segment / guanyl-nucleotide exchange factor activity / G protein-coupled receptor binding / G-protein beta/gamma-subunit complex binding / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / photoreceptor disc membrane / microtubule cytoskeleton organization / GDP binding / heterotrimeric G-protein complex / cell-cell junction / gene expression / G protein-coupled receptor signaling pathway / Golgi membrane / GTPase activity / GTP binding / protein kinase binding / zinc ion binding / identical protein binding / membrane / metal ion binding / plasma membrane / cytoplasm 類似検索 - 分子機能
Rhodopsin, N-terminal / Amino terminal of the G-protein receptor rhodopsin / Rhodopsin / Opsin / Visual pigments (opsins) retinal binding site / Visual pigments (opsins) retinal binding site. / Rhopdopsin 7-helix transmembrane proteins / Rhodopsin 7-helix transmembrane proteins / G-protein alpha subunit, group I / Serpentine type 7TM GPCR chemoreceptor Srsx ...Rhodopsin, N-terminal / Amino terminal of the G-protein receptor rhodopsin / Rhodopsin / Opsin / Visual pigments (opsins) retinal binding site / Visual pigments (opsins) retinal binding site. / Rhopdopsin 7-helix transmembrane proteins / Rhodopsin 7-helix transmembrane proteins / G-protein alpha subunit, group I / Serpentine type 7TM GPCR chemoreceptor Srsx / G-alpha domain profile. / Guanine nucleotide binding protein (G-protein), alpha subunit / G protein alpha subunit, helical insertion / G-protein alpha subunit / G protein alpha subunit / G-protein coupled receptors family 1 signature. / G protein-coupled receptor, rhodopsin-like / GPCR, rhodopsin-like, 7TM / G-protein coupled receptors family 1 profile. / 7 transmembrane receptor (rhodopsin family) / Up-down Bundle / P-loop containing nucleoside triphosphate hydrolase / Mainly Alpha 類似検索 - ドメイン・相同性
ACETATE ION / PALMITIC ACID / RETINAL / Rhodopsin / Guanine nucleotide-binding protein G(t) subunit alpha-1 / Guanine nucleotide-binding protein G(t) subunit alpha-3 類似検索 - 構成要素
ENGINEERED RESIDUE IN CHAIN A, ASN 2 TO CYS ENGINEERED RESIDUE IN CHAIN A, MET 257 TO TYR ...ENGINEERED RESIDUE IN CHAIN A, ASN 2 TO CYS ENGINEERED RESIDUE IN CHAIN A, MET 257 TO TYR ENGINEERED RESIDUE IN CHAIN A, ASP 282 TO CYS ENGINEERED RESIDUE IN CHAIN B, LYS 345 TO LEU
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 3
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試料調製
結晶
マシュー密度: 7.96 Å3/Da / 溶媒含有率: 84.43 % / 解説: NONE
結晶化
pH: 4.5 詳細: 3.0-3.4 M AMMONIUM SULPHATE, 100 MM SODIUM ACETATE PH 4.5
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データ収集
回折
平均測定温度: 100 K
放射光源
由来: シンクロトロン / サイト: SLS / ビームライン: X06SA / 波長: 1
検出器
タイプ: DECTRIS PILATUS 6M / 検出器: PIXEL
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1 Å / 相対比: 1
反射
解像度: 3.3→45 Å / Num. obs: 17649 / % possible obs: 94.8 % / Observed criterion σ(I): -3 / 冗長度: 5.7 % / Biso Wilson estimate: 75.56 Å2 / Rmerge(I) obs: 0.18 / Net I/σ(I): 8.5
反射 シェル
解像度: 3.3→3.48 Å / 冗長度: 2.9 % / Rmerge(I) obs: 0.66 / Mean I/σ(I) obs: 1.8 / % possible all: 65.4