+Open data
-Basic information
Entry | Database: PDB / ID: 3zyk | ||||||
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Title | Structure of CALM (PICALM) ANTH domain | ||||||
Components | PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN | ||||||
Keywords | ENDOCYTOSIS / ENDOBREVIN / SYNAPTOBREVIN / VAMP2 / VAMP3 / AP180 / PLASMA MEMBRANE / ADAPTOR PROTEIN | ||||||
Function / homology | Function and homology information regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process / negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process / membrane bending / RND3 GTPase cycle / vesicle cargo loading / endosome to plasma membrane transport vesicle / synaptic vesicle budding from presynaptic endocytic zone membrane / 1-phosphatidylinositol binding / regulation of terminal button organization / positive regulation of synaptic vesicle clustering ...regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process / negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process / membrane bending / RND3 GTPase cycle / vesicle cargo loading / endosome to plasma membrane transport vesicle / synaptic vesicle budding from presynaptic endocytic zone membrane / 1-phosphatidylinositol binding / regulation of terminal button organization / positive regulation of synaptic vesicle clustering / regulation of protein transport / extrinsic component of presynaptic endocytic zone membrane / positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process / regulation of synaptic vesicle transport / Golgi Associated Vesicle Biogenesis / amyloid-beta clearance by transcytosis / clathrin heavy chain binding / clathrin coat of coated pit / regulation of vesicle size / synaptic vesicle maturation / negative regulation of receptor-mediated endocytosis / negative regulation of protein localization to cell surface / clathrin coat assembly / Cargo recognition for clathrin-mediated endocytosis / positive regulation of synaptic vesicle endocytosis / Clathrin-mediated endocytosis / vesicle budding from membrane / positive regulation of dendrite extension / clathrin-dependent endocytosis / negative regulation of protein localization to plasma membrane / parallel fiber to Purkinje cell synapse / dendrite morphogenesis / neurofibrillary tangle / clathrin-coated vesicle / regulation of synaptic vesicle endocytosis / endosomal transport / low-density lipoprotein particle receptor binding / clathrin binding / positive regulation of axonogenesis / positive regulation of amyloid-beta formation / hemopoiesis / regulation of endocytosis / synaptic vesicle endocytosis / clathrin-coated pit / vesicle-mediated transport / phosphatidylinositol-4,5-bisphosphate binding / axonogenesis / receptor-mediated endocytosis / SNARE binding / Schaffer collateral - CA1 synapse / tau protein binding / receptor internalization / small GTPase binding / SH3 domain binding / multicellular organismal-level iron ion homeostasis / endocytosis / regulation of protein localization / synaptic vesicle / presynaptic membrane / postsynapse / postsynaptic membrane / intracellular iron ion homeostasis / vesicle / postsynaptic density / learning or memory / early endosome / endosome / negative regulation of gene expression / neuronal cell body / positive regulation of DNA-templated transcription / Golgi apparatus / cell surface / membrane / identical protein binding / nucleus / plasma membrane Similarity search - Function | ||||||
Biological species | RATTUS NORVEGICUS (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.8 Å | ||||||
Authors | Miller, S.E. / Sahlender, D.A. / Graham, S.C. / Honing, S. / Robinson, M.S. / Peden, A.A. / Owen, D.J. | ||||||
Citation | Journal: Cell / Year: 2011 Title: The molecular basis for the endocytosis of small R-SNAREs by the clathrin adaptor CALM. Authors: Miller, S.E. / Sahlender, D.A. / Graham, S.C. / Honing, S. / Robinson, M.S. / Peden, A.A. / Owen, D.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3zyk.cif.gz | 237.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3zyk.ent.gz | 191.9 KB | Display | PDB format |
PDBx/mmJSON format | 3zyk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zy/3zyk ftp://data.pdbj.org/pub/pdb/validation_reports/zy/3zyk | HTTPS FTP |
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-Related structure data
Related structure data | 3zylC 3zymC 1hf8S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
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-Components
#1: Protein | Mass: 33528.551 Da / Num. of mol.: 2 / Fragment: ANTH DOMAIN, RESIDUES 1-289 Source method: isolated from a genetically manipulated source Source: (gene. exp.) RATTUS NORVEGICUS (Norway rat) / Plasmid: PGEX 4T2 CALMANTH(1-289) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / Variant (production host): PLYSS / References: UniProt: O55012 #2: Water | ChemComp-HOH / | Sequence details | N-TERMINAL GSPIGIH SEQUENCE IS RESIDUAL FROM AFFINITY TAG AND CLONING. | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 52.35 % / Description: NONE |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop Details: CRYSTALS WERE GROWN IN SITTING DROPS CONTAINING EQUAL AMOUNTS (200-400 NL) OF PROTEIN (15 MG/ML IN 20 MM HEPES PH 7.4, 120 MM NACL, 4 MM DTT) AND RESERVOIR SOLUTION (100 MM BIS TRIS PROPANE, ...Details: CRYSTALS WERE GROWN IN SITTING DROPS CONTAINING EQUAL AMOUNTS (200-400 NL) OF PROTEIN (15 MG/ML IN 20 MM HEPES PH 7.4, 120 MM NACL, 4 MM DTT) AND RESERVOIR SOLUTION (100 MM BIS TRIS PROPANE, 200 MM SODIUM MALONATE, 20% (W/V) PEG 3350) AND EQUILIBRATED AGAINST 80 UL OF RESERVOIR SOLUTION AT 16C. CRYSTALS WERE CRYOPROTECTED BY BRIEF IMMERSION (5-60 S) IN RESERVOIR SOLUTION SUPPLEMENTED WITH 25% GLYCEROL AND WERE RAPIDLY CRYOCOOLED IN LIQUID N2 OR A 100 K STREAM OF N2 GAS. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.9763 |
Detector | Type: ADSC CCD / Detector: CCD / Date: Feb 7, 2010 / Details: MIRRORS |
Radiation | Monochromator: DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→73.5 Å / Num. obs: 64198 / % possible obs: 98.9 % / Observed criterion σ(I): -3 / Redundancy: 14.1 % / Biso Wilson estimate: 25.84 Å2 / Rmerge(I) obs: 0.1 / Net I/σ(I): 14.4 |
Reflection shell | Resolution: 1.8→1.85 Å / Redundancy: 14.5 % / Rmerge(I) obs: 0.86 / Mean I/σ(I) obs: 3.1 / % possible all: 98.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1HF8 Resolution: 1.8→40.009 Å / SU ML: 0.2 / σ(F): 1.34 / Phase error: 19.41 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 50.677 Å2 / ksol: 0.356 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 35.69 Å2
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Refinement step | Cycle: LAST / Resolution: 1.8→40.009 Å
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Refine LS restraints |
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Refine LS restraints NCS |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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