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Open data
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Basic information
Entry | Database: PDB / ID: 3w9b | ||||||
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Title | Crystal structure of Candida antarctica lipase B with anion-tag | ||||||
![]() | Lipase B | ||||||
![]() | HYDROLASE / LIPASE (CARBOXYLIC ESTERASE) | ||||||
Function / homology | ![]() triacylglycerol lipase / triglyceride lipase activity / lipid catabolic process Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kim, S.K. / Lee, H.H. / Park, Y.C. / Jeon, S.T. / Son, S.H. / Min, W.K. / Seo, J.H. | ||||||
![]() | ![]() Title: Anion tags improve extracellular production of Candida antarctica lipase B in Escherichia coli Authors: Kim, S.K. / Lee, H.H. / Park, Y.C. / Jeon, S.T. / Son, S.H. / Min, W.K. / Seo, J.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 469.3 KB | Display | ![]() |
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PDB format | ![]() | 389.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1008.4 KB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 50.3 KB | Display | |
Data in CIF | ![]() | 69.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1tcaS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 34274.465 Da / Num. of mol.: 4 / Fragment: UNP residues 26-342 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-PE8 / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.09 Å3/Da / Density % sol: 60.18 % |
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Crystal grow | Temperature: 297 K / Method: vapor diffusion, sitting drop / pH: 4.2 Details: 0.1M phosphate-citrate, 40% PEG600, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Apr 25, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→20 Å / Num. obs: 36843 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 |
Reflection shell | Resolution: 2.9→2.95 Å / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1TCA Resolution: 2.9→19.8 Å / Cor.coef. Fo:Fc: 0.901 / Cor.coef. Fo:Fc free: 0.856 / SU B: 31.377 / SU ML: 0.306 / Cross valid method: THROUGHOUT / ESU R Free: 0.415 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 49.268 Å2
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Refinement step | Cycle: LAST / Resolution: 2.9→19.8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.9→2.974 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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