[English] 日本語
Yorodumi- PDB-1tca: THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF T... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1tca | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA | |||||||||
Components | LIPASE | |||||||||
Keywords | HYDROLASE(CARBOXYLIC ESTERASE) | |||||||||
Function / homology | triacylglycerol lipase / triglyceride lipase activity / lipid catabolic process / Alpha/Beta hydrolase fold, catalytic domain / Alpha/Beta hydrolase fold / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta / Lipase B Function and homology information | |||||||||
Biological species | Candida antarctica (fungus) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 1.55 Å | |||||||||
Authors | Uppenberg, J. / Jones, T.A. | |||||||||
Citation | Journal: Structure / Year: 1994 Title: The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica. Authors: Uppenberg, J. / Hansen, M.T. / Patkar, S. / Jones, T.A. #1: Journal: J.Mol.Biol. / Year: 1994 Title: Crystallization and Preliminary X-Ray Studies of Lipase B from Candida Antarctica Authors: Uppenberg, J. / Patkar, S. / Bergfors, T. / Jones, T.A. | |||||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1tca.cif.gz | 72.6 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1tca.ent.gz | 55.7 KB | Display | PDB format |
PDBx/mmJSON format | 1tca.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tc/1tca ftp://data.pdbj.org/pub/pdb/validation_reports/tc/1tca | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Atom site foot note | 1: CIS PROLINE - PRO 70 / 2: CIS PROLINE - PRO 192 |
-Components
#1: Protein | Mass: 33040.238 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Candida antarctica (fungus) / References: UniProt: P41365, triacylglycerol lipase |
---|---|
#2: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
#3: Water | ChemComp-HOH / |
Sequence details | THE SEQUENCE HAS NOT BEEN REPORTED. IT WAS DERIVED FROM THE DNA SEQUENCE OF J.UPPENBERG ET AL., ...THE SEQUENCE HAS NOT BEEN REPORTED. IT WAS DERIVED FROM THE DNA SEQUENCE OF J.UPPENBERG ET AL., 1994, LISTED IN THE JRNL REFERENCE ABOVE. |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.08 % | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS pH: 3.6 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Radiation | Scattering type: x-ray |
---|---|
Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.55 Å / Lowest resolution: 100 Å / Num. obs: 37486 / % possible obs: 95 % / Num. measured all: 140416 / Rmerge(I) obs: 0.04 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 1.55→7.5 Å / σ(F): 2 /
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.55→7.5 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.157 / Rfactor Rwork: 0.157 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 1.1 |