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- PDB-1hfb: Crystal structure of the tyrosine-regulated 3-deoxy-D-arabino-hep... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1hfb | ||||||
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Title | Crystal structure of the tyrosine-regulated 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Saccharomyces cerevisiae complexed with phosphoenolpyruvate | ||||||
![]() | TYROSINE-REGULATED 3-DEOXY-D-ARABINO-HEPTULOSONATE-7-PHOSPHATE SYNTHASE | ||||||
![]() | LYASE / BETA-ALPHA-BARREL | ||||||
Function / homology | ![]() 3-deoxy-7-phosphoheptulonate synthase / 3-deoxy-7-phosphoheptulonate synthase activity / chorismate biosynthetic process / aromatic amino acid family biosynthetic process / amino acid biosynthetic process / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schneider, T.R. / Hartmann, M. / Braus, G.H. | ||||||
![]() | ![]() Title: Evolution of Feedback-Inhibited Beta /Alpha Barrel Isoenzymes by Gene Duplication and a Single Mutation Authors: Hartmann, M. / Schneider, T.R. / Pfeil, A. / Heinrich, G. / Lipscomb, W. / Braus, G.H. | ||||||
History |
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Remark 700 | SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS AB, BA, CB, DA, EB, FA, GB, AND HA BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS AB, BA, CB, DA, EB, FA, GB, AND HA BELOW ARE ACTUALLY 8-STRANDED BARRELS. THESE ARE REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 530 KB | Display | ![]() |
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PDB format | ![]() | 436.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1qr7S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given / Matrix: (1),Details | THE 8 MOLECULES CONTAINED IN THE ASSYMETRIC UNIT AREARRANGED IN TWO TETRAMERS (DIMER OF DIMERS) WITH APPROXIMATE222 POINT SYMMETRY. THE TETRAMERS HAVE VERY SIMILAR ORIENTATIONS,WITH THEIR CENTERS OF MASS BEING RELATED BY ACRYSTALLOGRAPHIC TRANSLATION OF (0.5,0.5,0). | |
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Components
#1: Protein | Mass: 39797.055 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: RH1326 / Production host: ![]() ![]() #2: Chemical | ChemComp-PEP / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 44.69 % | ||||||||||||||||||||||||
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Crystal grow | pH: 9 / Details: pH 9.00 | ||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 21 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 1, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9076 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→40 Å / Num. obs: 210365 / % possible obs: 96.3 % / Observed criterion σ(I): -3 / Redundancy: 2.01 % / Rmerge(I) obs: 0.037 |
Reflection | *PLUS Redundancy: 2.9 % / Num. measured all: 423490 |
Reflection shell | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 1.97 Å / % possible obs: 89.3 % / Redundancy: 1.2 % / Rmerge(I) obs: 0.354 / Mean I/σ(I) obs: 2.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: CHAINS A AND B OF PDB ENTRY 1QR7 Resolution: 1.9→40 Å / Data cutoff high absF: 10000 / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 41.9802 Å2 / ksol: 0.34361 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.9→40 Å
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Refine LS restraints |
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Xplor file |
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Refinement | *PLUS Rfactor Rfree: 0.261 / Rfactor Rwork: 0.208 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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