ソフトウェア 名称 バージョン 分類 NB SCALEPACKデータスケーリング Show REFMAC5.5.0109精密化 Show PDB_EXTRACT3.11 データ抽出 Show SERGUIデータ収集 HKL-2000データ削減 HKL-2000データスケーリング MOLREP位相決定
精密化 構造決定の手法 : 分子置換開始モデル : PDB ENTRY 3FZI解像度 : 1.9→50 Å / Cor.coef. Fo :Fc : 0.958 / Cor.coef. Fo :Fc free : 0.949 / Occupancy max : 1 / Occupancy min : 0.3 / SU B : 4.559 / SU ML : 0.063 / 交差検証法 : THROUGHOUT / σ(F) : 0 / ESU R : 0.112 / ESU R Free : 0.104 / 立体化学のターゲット値 : MAXIMUM LIKELIHOODRfactor 反射数 %反射 Selection details Rfree 0.1924 3265 5.1 % RANDOM Rwork 0.1719 - - - obs 0.173 64530 99.57 % -
溶媒の処理 イオンプローブ半径 : 0.8 Å / 減衰半径 : 0.8 Å / VDWプローブ半径 : 1.4 Å / 溶媒モデル : MASK原子変位パラメータ Biso max : 83.43 Å2 / Biso mean : 27.027 Å2 / Biso min : 2 Å2 Baniso -1 Baniso -2 Baniso -3 1- -0.1 Å2 -0.05 Å2 0 Å2 2- - -0.1 Å2 0 Å2 3- - - 0.15 Å2
精密化ステップ サイクル : LAST / 解像度 : 1.9→50 Åタンパク質 核酸 リガンド 溶媒 全体 原子数 4258 0 59 381 4698
拘束条件 Refine-ID タイプ Dev ideal Dev ideal target 数 X-RAY DIFFRACTION r_bond_refined_d0.009 0.022 4477 X-RAY DIFFRACTION r_angle_refined_deg1.133 1.947 6007 X-RAY DIFFRACTION r_dihedral_angle_1_deg6.148 5 521 X-RAY DIFFRACTION r_dihedral_angle_2_deg35.132 22.798 243 X-RAY DIFFRACTION r_dihedral_angle_3_deg14.06 15 772 X-RAY DIFFRACTION r_dihedral_angle_4_deg15.304 15 47 X-RAY DIFFRACTION r_chiral_restr0.083 0.2 598 X-RAY DIFFRACTION r_gen_planes_refined0.004 0.021 3483 X-RAY DIFFRACTION r_mcbond_it0.394 1.5 2579 X-RAY DIFFRACTION r_mcangle_it0.767 2 4167 X-RAY DIFFRACTION r_scbond_it1.384 3 1898 X-RAY DIFFRACTION r_scangle_it2.308 4.5 1840
LS精密化 シェル 解像度 : 1.9→1.949 Å / Total num. of bins used : 20 Rfactor 反射数 %反射 Rfree 0.258 233 - Rwork 0.214 4532 - all - 4765 - obs - 4765 99.75 %
精密化 TLS 手法 : refined / Refine-ID : X-RAY DIFFRACTION
大きな表を表示 (25 x 27) 大きな表を隠す ID L11 (°2 )L12 (°2 )L13 (°2 )L22 (°2 )L23 (°2 )L33 (°2 )S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2 )T12 (Å2 )T13 (Å2 )T22 (Å2 )T23 (Å2 )T33 (Å2 )Origin x (Å)Origin y (Å)Origin z (Å)1 2.273 0.9536 -0.3082 3.4672 0.2529 3.4071 -0.0443 0.0074 -0.1113 -0.1693 0.0335 -0.0941 0.0744 0.0724 0.0108 0.0543 0.0001 -0.0218 0.0429 -0.0119 0.0824 -9.714 20.635 -3.454 2 6.4134 0.0887 -2.3885 6.0412 -2.4815 7.9719 -0.0099 0.0303 -0.2287 -0.0223 0.1098 -0.2751 0.0858 0.3332 -0.0999 0.0719 0.0016 -0.0124 0.0874 -0.0469 0.1767 -4.258 14.636 -3.726 3 1.8952 1.2215 0.0607 3.3718 1.1164 1.7394 -0.161 0.261 -0.0601 -0.5403 0.2027 -0.0949 -0.1876 0.154 -0.0417 0.1367 -0.0353 -0.0173 0.1287 -0.0256 0.1277 -11.183 21.195 -11.507 4 6.9206 2.0135 -6.0486 2.8093 -2.0429 16.7521 -0.0273 0.5629 -0.2886 -0.4945 0.1782 -0.2337 -0.2426 0.0759 -0.151 0.2266 -0.0115 0.0453 0.1265 -0.0546 0.1885 -11.785 12.203 -13.113 5 3.0238 2.7895 0.0865 7.1303 0.5194 2.8174 -0.2051 0.2864 0.0766 -0.7206 0.203 -0.0983 -0.0986 0.0448 0.0021 0.1398 -0.0313 -0.0128 0.1382 -0.052 0.1123 -16.633 24.767 -14.84 6 1.8691 0.4175 0.2802 3.695 0.3547 1.285 -0.0999 0.1215 0.092 -0.2803 0.0071 0.0926 -0.2245 -0.0361 0.0928 0.143 0.0014 -0.0401 0.0793 -0.021 0.0788 -20.968 27.714 -9.975 7 3.6393 2.3309 0.1914 7.3619 0.7589 2.62 -0.1146 0.1432 0.0699 -0.2731 -0.0251 -0.0718 -0.2184 0.076 0.1396 0.0939 0.0303 -0.0546 0.0906 -0.0091 0.0462 -18.075 32.769 -3.242 8 6.3257 3.6072 -0.5565 4.519 -0.0018 2.7537 -0.1649 0.1941 0.4911 -0.3778 -0.009 0.2501 -0.4483 -0.0555 0.1739 0.1999 0.0333 -0.096 0.0659 0 0.1416 -20.107 42.488 -4.568 9 5.6003 1.6123 0.0985 2.5383 0.3185 0.7215 -0.0762 0.1444 -0.0004 -0.0684 -0.0924 0.296 -0.0892 -0.2034 0.1686 0.0938 0.0092 -0.0368 0.133 -0.0653 0.1029 -26.788 30.226 1.536 10 2.2957 -1.9277 0.3597 3.9585 -0.1053 3.4477 -0.0779 -0.0937 0.4902 0.1056 -0.0367 -0.275 -0.3303 0.1724 0.1146 0.1203 -0.0276 -0.0454 0.0673 -0.0399 0.1679 -6.711 41.981 10.459 11 1.8341 0.0558 0.42 1 0.3993 0.9156 -0.0211 -0.1199 0.1114 0.0135 -0.0498 0.1014 -0.1091 -0.0389 0.071 0.0907 -0.0103 -0.0237 0.0902 -0.0362 0.0815 -14.729 35.707 9.546 12 30.0779 3.4795 12.5406 0.2275 -3.4914 7.7993 -0.4972 3.2182 0.5252 -0.0477 0.1277 -0.148 -0.1125 1.6744 0.3695 0.221 -0.0892 0.0369 0.5543 0.0323 0.1788 6.474 34.15 0.986 13 3.9653 1.015 0.8723 1.4952 0.2819 0.8225 -0.0464 0.0127 0.0348 0.0177 -0.0508 -0.0014 -0.0473 0.0504 0.0972 0.0791 -0.0159 -0.0117 0.0524 -0.0136 0.0632 -9.973 31.23 7.122 14 4.8881 -0.1571 -1.1601 1.2662 0.644 3.0525 -0.0457 -0.2995 -0.3966 0.1178 -0.0846 0.0689 0.1762 0.0349 0.1303 0.0906 -0.0113 -0.0049 0.054 -0.0229 0.1068 -13.001 21.316 7.193 15 1.6932 -0.891 0.7244 3.505 1.6801 2.361 -0.132 -0.2813 0.1221 0.5633 -0.2103 0.3262 0.3547 -0.4885 0.3422 0.2276 -0.0159 0.0624 0.2466 -0.1591 0.2103 -29.823 44.172 26.262 16 3.9459 -5.6345 -1.0007 9.5246 4.5083 7.4906 -0.1986 -0.3815 -0.4415 1.2795 -0.5739 0.988 1.1792 -1.05 0.7725 0.6289 -0.3973 0.3049 0.6946 -0.323 0.4 -33.856 39.009 30.083 17 0.8846 -0.7529 -1.7045 3.95 2.4613 6.9266 -0.0552 -0.0382 0.1063 0.1115 -0.4167 0.5616 -0.0839 -0.7498 0.4719 0.133 0.0597 -0.0215 0.2757 -0.2075 0.3063 -31.246 47.873 20.565 18 9.082 0.4281 -1.8294 5.3523 5.8124 19.2104 -0.0947 0.0365 0.0559 0.1509 -0.997 1.1143 0.6103 -2.4959 1.0916 0.0669 -0.0527 0.0429 0.6265 -0.383 0.4686 -40.851 43.75 20.547 19 9.4657 -1.454 -11.5694 7.4475 2.57 25.4336 -0.0464 0.8904 0.0735 -1.088 -1.3244 0.921 -1.4483 -1.8099 1.3708 0.3151 0.3128 -0.3406 0.4627 -0.442 0.5255 -32.009 52.628 14.4 20 7.8109 -2.2969 -6.9499 4.2626 4.9227 13.3139 -0.3208 0.4922 0.0877 -0.2284 -0.2952 0.3852 -0.0933 -0.8355 0.616 0.1639 0.0438 -0.0778 0.2045 -0.1429 0.2461 -27.954 50.023 15.054 21 8.6663 1.1709 -0.0863 5.1023 0.5547 6.4983 -0.5863 -0.1766 1.1545 -0.2723 -0.2817 0.488 -0.8183 -0.9858 0.868 0.3141 0.2391 -0.2808 0.2922 -0.324 0.4604 -32.09 59.923 20.736 22 3.6077 -1.4148 -0.6561 3.1204 2.122 3.2649 -0.1889 -0.078 0.4902 -0.1801 -0.0324 -0.1067 -0.4323 -0.1686 0.2212 0.2571 0.0743 -0.1392 0.0887 -0.1153 0.298 -21.41 59.31 20.549 23 2.0303 -1.1085 1.2108 10.3733 0.4916 3.8449 -0.4709 -0.4298 0.5008 0.028 -0.1605 -0.2195 -0.7324 -0.5799 0.6314 0.3399 0.2381 -0.2685 0.2485 -0.3271 0.4572 -26.642 67.15 30.419 24 2.2864 -1.0553 0.2324 4.6331 1.4683 3.7724 -0.2147 -0.2121 0.5045 0.2041 0.4601 -0.6924 0.0763 0.5266 -0.2454 0.1404 0.0965 -0.16 0.1916 -0.2273 0.3375 -7.129 53.236 27.713 25 1.8087 -1.2387 -0.7511 6.6522 3.4679 1.7937 -0.373 -0.4554 0.3066 0.5477 0.4043 -0.2075 0.285 0.1577 -0.0312 0.3247 0.1563 -0.1324 0.2393 -0.1831 0.1953 -15.036 50.557 34.561 26 2.9306 -2.7879 -1.2927 5.3995 1.6647 2.584 -0.2608 -0.2419 0.2633 0.5719 0.1794 -0.3231 0.4965 0.0447 0.0814 0.3202 0.1174 -0.1153 0.1865 -0.1445 0.1638 -15.81 47.819 31.946 27 0.9722 -0.9142 0.3085 9.2589 5.6206 7.143 -0.3918 -0.3591 0.0574 1.4311 -0.1246 0.5684 0.86 -0.489 0.5165 0.4277 0.0776 0.0324 0.2675 -0.164 0.1548 -25.635 49.104 36.742
精密化 TLSグループ 大きな表を表示 (5 x 27) 大きな表を隠す ID Refine-ID Refine TLS-ID Auth asym-ID Auth seq-ID 1 X-RAY DIFFRACTION 1 A2 - 18 2 X-RAY DIFFRACTION 2 A19 - 30 3 X-RAY DIFFRACTION 3 A31 - 48 4 X-RAY DIFFRACTION 4 A49 - 56 5 X-RAY DIFFRACTION 5 A57 - 65 6 X-RAY DIFFRACTION 6 A66 - 102 7 X-RAY DIFFRACTION 7 A103 - 119 8 X-RAY DIFFRACTION 8 A120 - 135 9 X-RAY DIFFRACTION 9 A136 - 153 10 X-RAY DIFFRACTION 10 A154 - 169 11 X-RAY DIFFRACTION 11 A170 - 206 12 X-RAY DIFFRACTION 12 A207 - 211 13 X-RAY DIFFRACTION 13 A212 - 229 14 X-RAY DIFFRACTION 14 A230 - 257 15 X-RAY DIFFRACTION 15 D2 - 19 16 X-RAY DIFFRACTION 16 D20 - 31 17 X-RAY DIFFRACTION 17 D32 - 44 18 X-RAY DIFFRACTION 18 D45 - 57 19 X-RAY DIFFRACTION 19 D58 - 65 20 X-RAY DIFFRACTION 20 D66 - 73 21 X-RAY DIFFRACTION 21 D74 - 91 22 X-RAY DIFFRACTION 22 D92 - 136 23 X-RAY DIFFRACTION 23 D137 - 150 24 X-RAY DIFFRACTION 24 D151 - 183 25 X-RAY DIFFRACTION 25 D184 - 210 26 X-RAY DIFFRACTION 26 D211 - 229 27 X-RAY DIFFRACTION 27 D230 - 257