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Yorodumi- PDB-3vhl: Crystal structure of the DHR-2 domain of DOCK8 in complex with Cd... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3vhl | ||||||
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Title | Crystal structure of the DHR-2 domain of DOCK8 in complex with Cdc42 (T17N mutant) | ||||||
Components |
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Keywords | SIGNALING PROTEIN / signal transduction / guanine nucleotide exchang factor / GTPase | ||||||
Function / homology | Function and homology information memory T cell proliferation / RHOJ GTPase cycle / CDC42 GTPase cycle / GBD domain binding / submandibular salivary gland formation / actin filament branching / RAC1 GTPase cycle / Golgi transport complex / positive regulation of pinocytosis / modification of synaptic structure ...memory T cell proliferation / RHOJ GTPase cycle / CDC42 GTPase cycle / GBD domain binding / submandibular salivary gland formation / actin filament branching / RAC1 GTPase cycle / Golgi transport complex / positive regulation of pinocytosis / modification of synaptic structure / endothelin receptor signaling pathway involved in heart process / Cdc42 protein signal transduction / cardiac neural crest cell migration involved in outflow tract morphogenesis / positive regulation of synapse structural plasticity / dendritic cell migration / storage vacuole / apolipoprotein A-I receptor binding / positive regulation of epithelial cell proliferation involved in lung morphogenesis / neuron fate determination / modulation by host of viral process / immunological synapse formation / organelle transport along microtubule / regulation of attachment of spindle microtubules to kinetochore / positive regulation of pseudopodium assembly / Inactivation of CDC42 and RAC1 / cardiac conduction system development / Factors involved in megakaryocyte development and platelet production / GTP-dependent protein binding / regulation of filopodium assembly / establishment of Golgi localization / leading edge membrane / neuropilin signaling pathway / positive regulation of intracellular protein transport / cell junction assembly / filopodium assembly / negative regulation of T cell apoptotic process / cellular response to chemokine / establishment of epithelial cell apical/basal polarity / regulation of modification of postsynaptic structure / dendritic spine morphogenesis / mitogen-activated protein kinase kinase kinase binding / embryonic heart tube development / thioesterase binding / regulation of stress fiber assembly / RHO GTPases activate KTN1 / regulation of lamellipodium assembly / nuclear migration / DCC mediated attractive signaling / adherens junction organization / sprouting angiogenesis / Wnt signaling pathway, planar cell polarity pathway / CD28 dependent Vav1 pathway / regulation of postsynapse organization / positive regulation of filopodium assembly / regulation of mitotic nuclear division / establishment or maintenance of cell polarity / phagocytosis, engulfment / RHOV GTPase cycle / small GTPase-mediated signal transduction / heart contraction / Myogenesis / RHOJ GTPase cycle / Golgi organization / RHOQ GTPase cycle / positive regulation of cytokinesis / cell leading edge / RHO GTPases activate PAKs / lamellipodium membrane / CDC42 GTPase cycle / RHOU GTPase cycle / macrophage differentiation / RHOG GTPase cycle / RHO GTPases Activate WASPs and WAVEs / RAC3 GTPase cycle / RAC2 GTPase cycle / RHO GTPases activate IQGAPs / spindle midzone / positive regulation of T cell migration / negative regulation of protein-containing complex assembly / phagocytic vesicle / positive regulation of lamellipodium assembly / positive regulation of substrate adhesion-dependent cell spreading / positive regulation of stress fiber assembly / GPVI-mediated activation cascade / RAC1 GTPase cycle / EPHB-mediated forward signaling / positive regulation of establishment of T cell polarity / substantia nigra development / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / guanyl-nucleotide exchange factor activity / small monomeric GTPase / G protein activity / filopodium / positive regulation of DNA replication / secretory granule / actin filament organization / integrin-mediated signaling pathway / RHO GTPases Activate Formins / regulation of actin cytoskeleton organization / FCGR3A-mediated phagocytosis Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.085 Å | ||||||
Authors | Hanawa-Suetsugu, K. / Kukimoto-Niino, M. / Nishizak, T. / Terada, T. / Shirouzu, M. / Fukui, Y. / Yokoyama, S. | ||||||
Citation | Journal: Blood / Year: 2012 Title: DOCK8 is a Cdc42 activator critical for interstitial dendritic cell migration during immune responses. Authors: Harada, Y. / Tanaka, Y. / Terasawa, M. / Pieczyk, M. / Habiro, K. / Katakai, T. / Hanawa-Suetsugu, K. / Kukimoto-Niino, M. / Nishizaki, T. / Shirouzu, M. / Duan, X. / Uruno, T. / Nishikimi, ...Authors: Harada, Y. / Tanaka, Y. / Terasawa, M. / Pieczyk, M. / Habiro, K. / Katakai, T. / Hanawa-Suetsugu, K. / Kukimoto-Niino, M. / Nishizaki, T. / Shirouzu, M. / Duan, X. / Uruno, T. / Nishikimi, A. / Sanematsu, F. / Yokoyama, S. / Stein, J.V. / Kinashi, T. / Fukui, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3vhl.cif.gz | 196.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3vhl.ent.gz | 155.7 KB | Display | PDB format |
PDBx/mmJSON format | 3vhl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vh/3vhl ftp://data.pdbj.org/pub/pdb/validation_reports/vh/3vhl | HTTPS FTP |
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-Related structure data
Related structure data | 2wm9S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 33787.785 Da / Num. of mol.: 1 / Fragment: DHR-2 domain (UNP RESIDUES 1787-2067) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Dock8 / Plasmid: PX101019-11 / Production host: cell-free protein synthsis (unknown) / References: UniProt: Q8C147 | ||
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#2: Protein | Mass: 21474.535 Da / Num. of mol.: 1 / Mutation: T17N, C188S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CDC42 / Plasmid: PX080214-12 / Production host: cell-free protein synthsis (unknown) / References: UniProt: P60953 | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 43.35 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 0.2M Potassium phosphate, 20% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NW12A / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Dec 15, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.085→30 Å / Num. obs: 28080 / % possible obs: 99.5 % / Observed criterion σ(I): -3 / Redundancy: 5.5 % / Rsym value: 0.06 / Net I/σ(I): 24.6 |
Reflection shell | Resolution: 2.09→2.16 Å / Redundancy: 5 % / Mean I/σ(I) obs: 4.96 / Rsym value: 0.338 / % possible all: 98.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2WM9 Resolution: 2.085→29.679 Å / SU ML: 0.55 / σ(F): 0 / Phase error: 22.44 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.95 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 47.677 Å2 / ksol: 0.387 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.085→29.679 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 14
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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