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- PDB-3syc: Crystal structure of the G protein-gated inward rectifier K+ chan... -

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Basic information

Entry
Database: PDB / ID: 3syc
TitleCrystal structure of the G protein-gated inward rectifier K+ channel GIRK2 (Kir3.2) D228N mutant
ComponentsG protein-activated inward rectifier potassium channel 2
KeywordsMETAL TRANSPORT / ion channel / potassium channel / inward rectification / sodium binding / PIP2 binding / G protein binding
Function / homology
Function and homology information


G-protein activated inward rectifier potassium channel activity / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / inward rectifier potassium channel activity / neuronal cell body membrane / parallel fiber to Purkinje cell synapse / potassium ion import across plasma membrane / G-protein alpha-subunit binding / regulation of ion transmembrane transport / integral component of presynaptic membrane ...G-protein activated inward rectifier potassium channel activity / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / inward rectifier potassium channel activity / neuronal cell body membrane / parallel fiber to Purkinje cell synapse / potassium ion import across plasma membrane / G-protein alpha-subunit binding / regulation of ion transmembrane transport / integral component of presynaptic membrane / axon / dendrite / cell surface / plasma membrane / cytoplasm
Similarity search - Function
Potassium channel, inwardly rectifying, Kir3.2 / G protein-activated inward rectifier potassium channel 1 / Inward rectifier potassium channel transmembrane domain / Potassium channel, inwardly rectifying, transmembrane domain / Potassium channel, inwardly rectifying, Kir / Inward rectifier potassium channel C-terminal domain / Inward rectifier potassium channel, C-terminal / Potassium channel, inwardly rectifying, Kir, cytoplasmic / Helix Hairpins - #70 / Immunoglobulin E-set ...Potassium channel, inwardly rectifying, Kir3.2 / G protein-activated inward rectifier potassium channel 1 / Inward rectifier potassium channel transmembrane domain / Potassium channel, inwardly rectifying, transmembrane domain / Potassium channel, inwardly rectifying, Kir / Inward rectifier potassium channel C-terminal domain / Inward rectifier potassium channel, C-terminal / Potassium channel, inwardly rectifying, Kir, cytoplasmic / Helix Hairpins - #70 / Immunoglobulin E-set / Helix Hairpins / Immunoglobulin-like / Sandwich / Orthogonal Bundle / Mainly Beta / Mainly Alpha
Similarity search - Domain/homology
: / G protein-activated inward rectifier potassium channel 2
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.41 Å
AuthorsWhorton, M.R. / MacKinnon, R.
CitationJournal: Cell(Cambridge,Mass.) / Year: 2011
Title: Crystal Structure of the Mammalian GIRK2 K(+) Channel and Gating Regulation by G Proteins, PIP(2), and Sodium.
Authors: Whorton, M.R. / Mackinnon, R.
History
DepositionJul 16, 2011Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 12, 2011Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: G protein-activated inward rectifier potassium channel 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)39,2967
Polymers39,0611
Non-polymers2356
Water0
1
A: G protein-activated inward rectifier potassium channel 2
hetero molecules

A: G protein-activated inward rectifier potassium channel 2
hetero molecules

A: G protein-activated inward rectifier potassium channel 2
hetero molecules

A: G protein-activated inward rectifier potassium channel 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)157,18228
Polymers156,2444
Non-polymers93824
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_545-x,-y-1,z1
crystal symmetry operation3_445-y-1/2,x-1/2,z1
crystal symmetry operation4_545y+1/2,-x-1/2,z1
Buried area26730 Å2
ΔGint-145 kcal/mol
Surface area49790 Å2
MethodPISA
Unit cell
Length a, b, c (Å)86.488, 86.488, 179.627
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number90
Space group name H-MP4212
Components on special symmetry positions
IDModelComponents
11A-501-

K

21A-502-

K

31A-503-

K

41A-504-

K

51A-505-

K

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Components

#1: Protein G protein-activated inward rectifier potassium channel 2 / GIRK-2 / Inward rectifier K(+) channel Kir3.2 / Potassium channel / inwardly rectifying subfamily J member 6


Mass: 39060.973 Da / Num. of mol.: 1 / Fragment: UNP residues 52-380 / Mutation: D228N
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Girk2, Kcnj6, Kcnj7, W / Plasmid: pPICZ / Production host: Pichia pastoris (fungus) / Strain (production host): SMD1163 / References: UniProt: P48542
#2: Chemical
ChemComp-K / POTASSIUM ION


Mass: 39.098 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: K
Sequence detailsUNP P48542 S260T, I313M, AND M344L ARE NATURAL VARIANTS.

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.3 Å3/Da / Density % sol: 71.39 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 6
Details: 50 mM sodium citrate, pH 6.0, 1 M sodium nitrate, 24-26% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: NSLS / Beamline: X29A / Wavelength: 1.075 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 2, 2010
RadiationMonochromator: Rosenbaum-Rock double crystal sagittal focusing
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.075 Å / Relative weight: 1
ReflectionResolution: 3.4→42.043 Å / Num. obs: 9865 / % possible obs: 99.5 % / Redundancy: 5.6 % / Rmerge(I) obs: 0.092 / Χ2: 1.344 / Net I/σ(I): 11.7
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique allΧ2Diffraction-ID% possible all
3.4-3.525.80.9319481.017199.9
3.52-3.665.80.6189731.09199.9
3.66-3.835.80.3739581.236199.9
3.83-4.035.80.2749711.3421100
4.03-4.285.70.1659711.609199.9
4.28-4.615.80.1089741.627199.9
4.61-5.085.60.0979971.66199.8
5.08-5.815.60.0849881.541199.8
5.81-7.325.50.07210071.243199.2
7.32-42.04350.04810781.062196.7

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Processing

Software
NameVersionClassificationNB
SCALEPACKdata scaling
REFMAC5.5.0109refinement
PDB_EXTRACT3.1data extraction
CBASSdata collection
DENZOdata reduction
MOLREPphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 2E4F
Resolution: 3.41→42.04 Å / Cor.coef. Fo:Fc: 0.903 / Cor.coef. Fo:Fc free: 0.921 / Occupancy max: 1 / Occupancy min: 0.13 / SU B: 49.323 / SU ML: 0.37 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.481 / Stereochemistry target values: MAXIMUM LIKELIHOOD
Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : RESIDUAL ONLY
RfactorNum. reflection% reflectionSelection details
Rfree0.2705 684 6.9 %RANDOM
Rwork0.2506 ---
obs0.252 9857 99.17 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK
Displacement parametersBiso max: 444.6 Å2 / Biso mean: 173.3819 Å2 / Biso min: 75.84 Å2
Baniso -1Baniso -2Baniso -3
1--0.56 Å20 Å20 Å2
2---0.56 Å20 Å2
3---1.11 Å2
Refinement stepCycle: LAST / Resolution: 3.41→42.04 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2366 0 6 0 2372
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0120.0222417
X-RAY DIFFRACTIONr_angle_refined_deg1.4531.9463295
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.7875307
X-RAY DIFFRACTIONr_dihedral_angle_2_deg34.85623.51194
X-RAY DIFFRACTIONr_dihedral_angle_3_deg19.42515376
X-RAY DIFFRACTIONr_dihedral_angle_4_deg17.0661511
X-RAY DIFFRACTIONr_chiral_restr0.0930.2393
X-RAY DIFFRACTIONr_gen_planes_refined0.0060.0211789
X-RAY DIFFRACTIONr_mcbond_it0.4861.51537
X-RAY DIFFRACTIONr_mcangle_it0.96122465
X-RAY DIFFRACTIONr_scbond_it1.4233880
X-RAY DIFFRACTIONr_scangle_it2.5924.5830
LS refinement shellResolution: 3.406→3.493 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.29 48 -
Rwork0.279 630 -
all-678 -
obs--94.83 %
Refinement TLS params.Method: refined / Origin x: 9.8009 Å / Origin y: -31.2644 Å / Origin z: 53.1924 Å
111213212223313233
T0.5963 Å2-0.0208 Å20.1445 Å2-0.6798 Å20.0293 Å2--0.2823 Å2
L4.3465 °20.2569 °2-0.2872 °2-3.3709 °21.5725 °2--3.6201 °2
S-0.0912 Å °1.5893 Å °-0.2563 Å °-1.2809 Å °-0.0136 Å °-0.3223 Å °-0.5141 Å °0.1996 Å °0.1048 Å °

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