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- PDB-3pux: Crystal Structure of an outward-facing MBP-Maltose transporter co... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3pux | |||||||||
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Title | Crystal Structure of an outward-facing MBP-Maltose transporter complex bound to ADP-BeF3 | |||||||||
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![]() | HYDROLASE/TRANSPORT PROTEIN / ATP Binding Cassette / Nucleotide Binding Domain / Substrate Binding Protein / ABC Transporter / importer / ATPase / ATP binding / Maltodextrin binding / transmembrane integral membrane / HYDROLASE-TRANSPORT PROTEIN complex | |||||||||
Function / homology | ![]() ABC-type maltose transporter / ABC-type maltose transporter activity / negative regulation of maltose transport / enzyme IIA-maltose transporter complex / negative regulation of transmembrane transport / detection of maltose stimulus / maltose transport complex / carbohydrate transport / carbohydrate transmembrane transporter activity / maltose binding ...ABC-type maltose transporter / ABC-type maltose transporter activity / negative regulation of maltose transport / enzyme IIA-maltose transporter complex / negative regulation of transmembrane transport / detection of maltose stimulus / maltose transport complex / carbohydrate transport / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / ATP-binding cassette (ABC) transporter complex / cell chemotaxis / outer membrane-bounded periplasmic space / DNA-binding transcription factor binding / periplasmic space / DNA damage response / ATP hydrolysis activity / ATP binding / membrane / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Oldham, M.L. / Chen, J. | |||||||||
![]() | ![]() Title: Snapshots of the maltose transporter during ATP hydrolysis. Authors: Oldham, M.L. / Chen, J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 778 KB | Display | ![]() |
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PDB format | ![]() | 634 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3puvC ![]() 3puwC ![]() 3rlfC ![]() 2r6gS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 2 types, 3 molecules EAB
#1: Protein | Mass: 41622.047 Da / Num. of mol.: 1 / Fragment: unp residues 27-396 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#4: Protein | Mass: 42184.535 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Maltose transport system permease protein ... , 2 types, 2 molecules FG
#2: Protein | Mass: 57052.898 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#3: Protein | Mass: 32246.227 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Sugars , 1 types, 1 molecules
#5: Polysaccharide | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose / alpha-maltose |
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-Non-polymers , 6 types, 269 molecules ![](data/chem/img/UMQ.gif)
![](data/chem/img/PGV.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/ADP.gif)
![](data/chem/img/BEF.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/PGV.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/ADP.gif)
![](data/chem/img/BEF.gif)
![](data/chem/img/HOH.gif)
#6: Chemical | ChemComp-UMQ / | ||||||||
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#7: Chemical | ChemComp-PGV / ( #8: Chemical | #9: Chemical | #10: Chemical | #11: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.92 Å3/Da / Density % sol: 68.59 % |
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Crystal grow | Temperature: 293 K / pH: 7.5 Details: 27% PEG 400, 0.1 M HEPES, 10 mM magnesium chloride, 50 mM sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: May 10, 2009 |
Radiation | Monochromator: DOUBLE CRYSTAL MONOCHROMATOR AND VERTICALLY FOCUSING MIRROR Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97965 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→20 Å / Num. obs: 91147 / % possible obs: 59.7 % / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.055 |
Reflection shell | Resolution: 2.2→2.28 Å / Redundancy: 2.5 % / Mean I/σ(I) obs: 1.9 / Rsym value: 0.451 / % possible all: 16.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2R6G Resolution: 2.3→19.81 Å / Cor.coef. Fo:Fc: 0.919 / Cor.coef. Fo:Fc free: 0.885 / SU B: 16.402 / SU ML: 0.181 / Cross valid method: THROUGHOUT / ESU R: 0.455 / ESU R Free: 0.297 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 48.32 Å2
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Refinement step | Cycle: LAST / Resolution: 2.3→19.81 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.3→2.36 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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