温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 詳細: Protein Solution (15 mg/ml RmlA Q83s protein, 10mM MOPS pH 7.5, 25mM dGTP) mixed in a 1:1 ratio with the well solution (10% MEPEG5K, 120mM MgCl2, 100mM Tris pH 8.5, 1mM Suramine) ...詳細: Protein Solution (15 mg/ml RmlA Q83s protein, 10mM MOPS pH 7.5, 25mM dGTP) mixed in a 1:1 ratio with the well solution (10% MEPEG5K, 120mM MgCl2, 100mM Tris pH 8.5, 1mM Suramine) Cryoprotected with 20% Ethylene Glycol, 10% MEPEG5K, 120mM MgCl2, 100mM Tris pH 8.5, 1mM Suramine, VAPOR DIFFUSION, HANGING DROP, temperature 298K