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Open data
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Basic information
Entry | Database: PDB / ID: 1mp3 | ||||||
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Title | L89T VARIANT OF S. ENTERICA RmlA | ||||||
![]() | GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE | ||||||
![]() | TRANSFERASE | ||||||
Function / homology | ![]() glucose-1-phosphate thymidylyltransferase / glucose-1-phosphate thymidylyltransferase activity / extracellular polysaccharide biosynthetic process / nucleotide binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Barton, W.A. / Biggins, J.B. / Jiang, J. / Thorson, J.T. / Nikolov, D.B. | ||||||
![]() | ![]() Title: Expanding pyrimidine diphosphosugar libraries via structure-based nucleotidylyltransferase engineering Authors: Barton, W.A. / Biggins, J.B. / Jiang, J. / Thorson, J.T. / Nikolov, D.B. | ||||||
History |
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Remark 999 | SEQUENCE Author states the sequence of the deposited model differs from the published sequence, ...SEQUENCE Author states the sequence of the deposited model differs from the published sequence, because there are confirmed natural mutations in the variant of Salmonella used in this entry. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 123.5 KB | Display | ![]() |
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PDB format | ![]() | 97.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 585.9 KB | Display | ![]() |
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Full document | ![]() | 600.2 KB | Display | |
Data in XML | ![]() | 15.2 KB | Display | |
Data in CIF | ![]() | 21.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1mp4C ![]() 1mp5C ![]() 1iinS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32415.045 Da / Num. of mol.: 2 / Mutation: L89T Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q9F7G8, UniProt: A0A6C6YZ99*PLUS, glucose-1-phosphate thymidylyltransferase #2: Chemical | ChemComp-TTP / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.61 Å3/Da / Density % sol: 52.83 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7.0, temperature 100K | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.4 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Date: Mar 29, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.072 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→30 Å / Num. obs: 33576 / Observed criterion σ(I): 0 |
Reflection | *PLUS Highest resolution: 2.2 Å / % possible obs: 95 % / Redundancy: 4 % / Rmerge(I) obs: 0.049 |
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Processing
Refinement | Method to determine structure: ![]() Starting model: PDB EMTRY 1IIN Resolution: 2.2→30 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.2→30 Å
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Refinement | *PLUS Highest resolution: 2.21 Å / Lowest resolution: 8 Å / % reflection Rfree: 10 % / Rfactor Rfree: 0.281 / Rfactor Rwork: 0.243 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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