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TitleExpanding the Nucleotide and Sugar 1-Phosphate Promiscuity of Nucleotidyltransferase RmlA via Directed Evolution.
Journal, issue, pagesJ. Biol. Chem., Vol. 286, Page 13235-13243, Year 2011
Publish dateNov 11, 2010 (structure data deposition date)
AuthorsMoretti, R. / Chang, A. / Peltier-Pain, P. / Bingman, C.A. / Phillips, G.N. / Thorson, J.S.
External linksJ. Biol. Chem. / PubMed:21317292
MethodsX-ray diffraction
Resolution2.4 - 2.6 Å
Structure data

PDB-3pkp:
Q83S Variant of S. Enterica RmlA with dATP
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-3pkq:
Q83D Variant of S. Enterica RmlA with dGTP
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-DTP:
2'-DEOXYADENOSINE 5'-TRIPHOSPHATE

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-DGT:
2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • salmonella enterica subsp. enterica serovar typhimurium (bacteria)
KeywordsTRANSFERASE / nucleotidylyltransferase / Directed evolution / Structural Genomics / PSI-2 / Protein Structure Initiative / Center for Eukaryotic Structural Genomics / CESG

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