[English] 日本語
Yorodumi- PDB-3mjh: Crystal Structure of Human Rab5A in complex with the C2H2 Zinc Fi... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3mjh | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal Structure of Human Rab5A in complex with the C2H2 Zinc Finger of EEA1 | ||||||
Components |
| ||||||
Keywords | PROTEIN TRANSPORT / PROTEIN-ZINC FINGER COMPLEX / BETA BETA ALPHA FOLD / BETA HAIRPIN / Rab5A GTPase / EEA1 | ||||||
Function / homology | Function and homology information serine-pyruvate aminotransferase complex / synaptic vesicle to endosome fusion / regulation of endosome size / postsynaptic early endosome / cytoplasmic side of early endosome membrane / Toll Like Receptor 9 (TLR9) Cascade / 1-phosphatidylinositol binding / synaptic vesicle recycling / axonal spine / amyloid-beta clearance by transcytosis ...serine-pyruvate aminotransferase complex / synaptic vesicle to endosome fusion / regulation of endosome size / postsynaptic early endosome / cytoplasmic side of early endosome membrane / Toll Like Receptor 9 (TLR9) Cascade / 1-phosphatidylinositol binding / synaptic vesicle recycling / axonal spine / amyloid-beta clearance by transcytosis / chemical synaptic transmission, postsynaptic / vesicle fusion / modulation by host of viral process / regulation of autophagosome assembly / GTP-dependent protein binding / regulation of filopodium assembly / RAB geranylgeranylation / early endosome to late endosome transport / RAB GEFs exchange GTP for GDP on RABs / early phagosome / TBC/RABGAPs / regulation of synaptic vesicle exocytosis / positive regulation of exocytosis / Synthesis of PIPs at the plasma membrane / canonical Wnt signaling pathway / endomembrane system / phagocytic vesicle / axon terminus / phagocytosis / somatodendritic compartment / Prevention of phagosomal-lysosomal fusion / ruffle / small monomeric GTPase / G protein activity / clathrin-coated endocytic vesicle membrane / regulation of long-term neuronal synaptic plasticity / intracellular protein transport / Schaffer collateral - CA1 synapse / terminal bouton / receptor internalization / recycling endosome / endocytosis / phagocytic vesicle membrane / GDP binding / melanosome / synaptic vesicle / actin cytoskeleton / Clathrin-mediated endocytosis / Factors involved in megakaryocyte development and platelet production / early endosome membrane / postsynapse / early endosome / calmodulin binding / endosome membrane / endosome / membrane raft / axon / intracellular membrane-bounded organelle / GTPase activity / dendrite / neuronal cell body / glutamatergic synapse / GTP binding / protein homodimerization activity / extracellular exosome / zinc ion binding / nucleoplasm / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.03 Å | ||||||
Authors | Mishra, A.K. / Eathiraj, S. / Lambright, D.G. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2010 Title: Structural basis for Rab GTPase recognition and endosome tethering by the C2H2 zinc finger of Early Endosomal Autoantigen 1 (EEA1). Authors: Mishra, A. / Eathiraj, S. / Corvera, S. / Lambright, D.G. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 3mjh.cif.gz | 100.5 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb3mjh.ent.gz | 75.9 KB | Display | PDB format |
PDBx/mmJSON format | 3mjh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mj/3mjh ftp://data.pdbj.org/pub/pdb/validation_reports/mj/3mjh | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
Unit cell |
|
-Components
-Protein / Protein/peptide , 2 types, 4 molecules ACBD
#1: Protein | Mass: 18801.445 Da / Num. of mol.: 2 / Fragment: residues 16-183 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RAB5, RAB5A / Plasmid: modified pET15b, modified pET28a,pGEX / Production host: Escherichia coli (E. coli) Strain (production host): K12, BL21 (DE3)Codon Plus RIL cells References: UniProt: P20339, small monomeric GTPase #2: Protein/peptide | Mass: 3678.005 Da / Num. of mol.: 2 / Fragment: C2H2-type, residues 36-69 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: Early Endosomal Antigen1(EEA1), EEA1, ZFYVE2 / Plasmid: modified pET15b / Production host: Escherichia coli (E. coli) Strain (production host): K12, BL21(DE3)Codon Plus RIL cells References: UniProt: Q15075 |
---|
-Non-polymers , 4 types, 318 molecules
#3: Chemical | #4: Chemical | #5: Chemical | #6: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 42.72 % |
---|---|
Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 18% PEG 4000, 50mM sodium acetate, 0.2M sodium-potassium phosphate, 10% glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
-Data collection
Diffraction | Mean temperature: 298 K |
---|---|
Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH3R / Wavelength: 1.5418 Å |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Dec 6, 2007 / Details: mirrors |
Radiation | Monochromator: Osmic Mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→50 Å / Num. obs: 27931 / % possible obs: 97.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 4.5 % / Biso Wilson estimate: 35.344 Å2 / Rsym value: 0.042 / Net I/σ(I): 44.7 |
-Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: mouse Rab5C Resolution: 2.03→20 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.917 / SU B: 3.351 / SU ML: 0.094 / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / ESU R: 0.275 / ESU R Free: 0.223 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 37.193 Å2
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.03→20 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.025→2.077 Å / Total num. of bins used: 20
|