- PDB-3l70: Cytochrome BC1 complex from chicken with trifloxystrobin bound -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 3l70
Title
Cytochrome BC1 complex from chicken with trifloxystrobin bound
Components
(Cytochrome b-c1 complex subunit Rieske, ...) x 2
(Mitochondrial ubiquinol-cytochrome c reductase ...) x 4
(Mitochondrial ubiquinol-cytochrome-c reductase complex core protein ...) x 2
Cytochrome b
Mitochondrial cytochrome c1, heme protein
Keywords
OXIDOREDUCTASE / CYTOCHROME BC1 / MEMBRANE PROTEIN / HEME PROTEIN / RIESKE IRON SULFUR PROTEIN / CYTOCHROME B / CYTOCHROME C1 / COMPLEX III / MITOCHONDRIAL PROCESSING PROTEIN / UBIQUINONE / TRIFLOXYSTROBIN OXIDOREDUCTASE / REDOX ENZYME RESPIRATORY CHAIN / ELECTRON TRANSPORT / HEME / INNER MEMBRANE / MEMBRANE / STROBILURINS BINDING / MITOCHONDRION / TRANSMEMBRANE / STIGMATELLIN / IRON / MITOCHONDRIAL INNER MEMBRANE / RESPIRATORY CHAIN / IRON-SULFUR / TRANSIT PEPTIDE / Metal-binding / Mitochondrion inner membrane / Transport / Disulfide bond
Function / homology
Function and homology information
Respiratory electron transport / quinol-cytochrome-c reductase / : / quinol-cytochrome-c reductase / ubiquinol-cytochrome-c reductase activity / mitochondrial electron transport, ubiquinol to cytochrome c / respiratory electron transport chain / 2 iron, 2 sulfur cluster binding / response to oxidative stress / electron transfer activity ...Respiratory electron transport / quinol-cytochrome-c reductase / : / quinol-cytochrome-c reductase / ubiquinol-cytochrome-c reductase activity / mitochondrial electron transport, ubiquinol to cytochrome c / respiratory electron transport chain / 2 iron, 2 sulfur cluster binding / response to oxidative stress / electron transfer activity / oxidoreductase activity / heme binding / mitochondrion / membrane / metal ion binding / cytoplasm Similarity search - Function
Journal: To be Published Title: Famoxadone and related inhibitors bind like methoxy acrylate inhibitors in the Qo site of the BC1 compl and fix the rieske iron-sulfur protein in a positio close to but distinct from that seen ...Title: Famoxadone and related inhibitors bind like methoxy acrylate inhibitors in the Qo site of the BC1 compl and fix the rieske iron-sulfur protein in a positio close to but distinct from that seen with stigmatellin and other "distal" Qo inhibitors. Authors: Huang, L. / Berry, E.A.
The deposited coordinates (20 chains plus hetero groups) make up the asymmetric unit which is the biological assembly. One other subunit of the biological assembly (Subunit 11) is lost during purification or crystallization and is not present in the deposited structure.
Mass: 18.015 Da / Num. of mol.: 19 / Source method: isolated from a natural source / Formula: H2O
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Details
Sequence details
THE COMPLETE SEQUENCE OF CHAIN I AND V IS ...THE COMPLETE SEQUENCE OF CHAIN I AND V IS MLSVAARSGPFAPYLSAAAHAVPGPLKALAPAALRAEKVVLDLKRPLLCRESMSGRSARRDLVAGISLNAPASVRY, UNP residues 1-76. THE N-TERMINUS IS DISORDERED.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 4.02 Å3/Da / Density % sol: 69.38 % Description: REMARK: THE DATA WAS COLLECTED IN TWO PASSES- HIGH RES PASS WAS INTEGRATED 60 TO 2.8 A, LOW RES 60 TO 3.26 A, AND BOTH PASSES WERE SCALED SIMULTANEOUSLY IN SCALEPACK. DISTANCE 400 MM FOR ...Description: REMARK: THE DATA WAS COLLECTED IN TWO PASSES- HIGH RES PASS WAS INTEGRATED 60 TO 2.8 A, LOW RES 60 TO 3.26 A, AND BOTH PASSES WERE SCALED SIMULTANEOUSLY IN SCALEPACK. DISTANCE 400 MM FOR HI RES, 700 MM FOR LOW RES PASS. RESOLUTION USED IN REFINEMENT WAS 25 TO 2.75 A.
Crystal grow
Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 6.77 Details: FINAL CONCENTRATIONS BEFORE DIFFUSION: 50 MM CACODYLATE, 9.4 MM TRISHCL, 10 MM MGCL2, 50 G/L GLYCEROL, 30 G/L PEG 3350DA, 0.23 MM EDTA, 0.47 G/L UNDECYL MALTOSIDE, 31 MM OCTYL GLUCOSIDE, PH ...Details: FINAL CONCENTRATIONS BEFORE DIFFUSION: 50 MM CACODYLATE, 9.4 MM TRISHCL, 10 MM MGCL2, 50 G/L GLYCEROL, 30 G/L PEG 3350DA, 0.23 MM EDTA, 0.47 G/L UNDECYL MALTOSIDE, 31 MM OCTYL GLUCOSIDE, PH 6.77, VAPOR DIFFUSION, SITTING DROP, temperature 277K
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Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: SYNCHROTRON / Site: ALS / Beamline: 5.0.2 / Wavelength: 1 / Wavelength: 1 Å
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