PDB-3l28: Crystal structure of Zaire Ebola VP35 interferon inhibitory domai...

ID or keywords:

Entry

Database: PDB / ID: 3l28

Title

Crystal structure of Zaire Ebola VP35 interferon inhibitory domain K339A mutant

Components

Polymerase cofactor VP35

Keywords

RNA BINDING PROTEIN / RNA BINDING DOMAIN / INTERFERON ANTIVIRAL EVASION / RNA REPLICATION / RNA-BINDING PROTEIN / TRANSCRIPTION / Host cytoplasm / Interferon antiviral system evasion / RNA-binding / Virion

Function / homology

Function and homology information

suppression by virus of host cytokine production / symbiont-mediated suppression of host defenses / symbiont-mediated suppression of host RNAi-mediated antiviral immune response / negative regulation of miRNA-mediated gene silencing / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF7 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IKBKE activity / suppression by virus of host type I interferon production / symbiont-mediated suppression of host antigen processing and presentation of peptide antigen via MHC class II / positive regulation of protein sumoylation / molecular sequestering activity ...
Similarity search - Function

Biological species

Zaire ebolavirus

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.4 Å

Authors

Leung, D.W. / Prins, K.C. / Borek, D.M. / Farahbakhsh, M. / Tufariello, J.M. / Ramanan, P. / Nix, J.C. / Helgeson, L.A. / Otwinowski, Z. / Honzatko, R.B. ...

Citation

Journal: Nat.Struct.Mol.Biol. / Year: 2010
Title: Structural basis for dsRNA recognition and interferon antagonism by Ebola VP35.
Authors: Leung, D.W. / Prins, K.C. / Borek, D.M. / Farahbakhsh, M. / Tufariello, J.M. / Ramanan, P. / Nix, J.C. / Helgeson, L.A. / Otwinowski, Z. / Honzatko, R.B. / Basler, C.F. / Amarasinghe, G.K.

History

Deposition	Dec 14, 2009	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Jan 26, 2010	Provider: repository / Type: Initial release
Revision 1.1	Jul 13, 2011	Group: Advisory / Version format compliance
Revision 1.2	Oct 13, 2021	Group: Database references / Derived calculations Category: database_2 / pdbx_struct_conn_angle ...database_2 / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
Revision 1.3	Sep 6, 2023	Group: Data collection / Refinement description Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.4	Nov 22, 2023	Group: Data collection / Category: chem_comp_atom / chem_comp_bond / Item: _chem_comp_atom.atom_id / _chem_comp_bond.atom_id_2
Revision 1.5	Oct 30, 2024	Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

Structure viewer	Molecule: MolmilJmol/JSmol

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Validation report

Summary document	3l28_validation.pdf.gz	480.4 KB	Display	wwPDB validaton report
Full document	3l28_full_validation.pdf.gz	485.3 KB	Display
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Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/l2/3l28 ftp://data.pdbj.org/pub/pdb/validation_reports/l2/3l28	HTTPS FTP

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Related structure data

Related structure data	3l25C 3l26C 3l27C 3fkeS 3l29 3l2a S: Starting model for refinement C: citing same article (ref.)
Similar structure data	6l62-d 6wgr-d 3c2g-1 5nfy-2 3c2g-2 6ekv-d 6q0i-d 6i3z-d 6q20-d 6q0h-d Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#178 - Oct 2014 Ebola Virus Proteins similarity (7)

Deposited unit

A: Polymerase cofactor VP35

B: Polymerase cofactor VP35

C: Polymerase cofactor VP35

D: Polymerase cofactor VP35

E: Polymerase cofactor VP35

F: Polymerase cofactor VP35

hetero molecules

87 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author

Unit cell

Length a, b, c (Å)	53.381, 91.880, 102.595
Angle α, β, γ (deg.)	90.00, 99.60, 90.00
Int Tables number	4
Space group name H-M	P12₁1

#1: Protein	Polymerase cofactor VP35 Mass: 14246.213 Da / Num. of mol.: 6 / Fragment: VP35 INTERFERON INHIBITORY DOMAIN / Mutation: K339A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Zaire ebolavirus / Strain: Mayinga-76 / Gene: VP35 / Plasmid: MODIFIED PET15B / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q05127
#2: Chemical	ChemComp-SO4 / SULFATE ION Mass: 96.063 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: SO₄
#3: Chemical	ChemComp-CL / CHLORIDE ION Mass: 35.453 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: Cl
#4: Chemical	ChemComp-NA / SODIUM ION Mass: 22.990 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: Na
#5: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 499 / Source method: isolated from a natural source / Formula: H₂O
Has protein modification	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.9 Å³/Da / Density % sol: 57.62 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 2.8 Details: 0.1M SODIUM CITRATE, 0.3M LITHIUM SULFATE, 13% PEG8000, pH 2.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.979 Å
Detector	Type: ADSC QUANTUM 315r / Detector: CCD / Date: Mar 20, 2009
Radiation	Monochromator: DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.979 Å / Relative weight: 1
Reflection	Resolution: 2.4→50 Å / Num. all: 38190 / Num. obs: 38190 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.8 % / R_merge(I) obs: 0.044 / Net I/σ(I): 29
Reflection shell	Resolution: 2.4→2.42 Å / Redundancy: 3.7 % / R_merge(I) obs: 0.433 / Mean I/σ(I) obs: 2.8 / % possible all: 100

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Processing

Software

Name	Version	Classification
HKL-3000		data collection
PHASER		phasing
REFMAC	5.3.0037	refinement
HKL-3000		data reduction
SCALEPACK		data scaling

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 3FKE

3fke

Resolution: 2.4→33.77 Å / Cor.coef. F_o:F_c: 0.926 / Cor.coef. F_o:F_c free: 0.864 / SU B: 14.97 / SU ML: 0.205 / TLS residual ADP flag: LIKELY RESIDUAL / Isotropic thermal model: ISOTROPIC / Cross valid method: THROUGHOUT / ESU R: 0.483 / ESU R Free: 0.303 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.26908	1720	5.1 %	RANDOM
R_work	0.19987	-	-	-
obs	0.20352	31958	87.91 %	-
all	-	31960	-	-

Solvent computation

Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK

Displacement parameters

B_iso mean: 9.914 Å²

	B_aniso -1	B_aniso -2	B_aniso -3
1-	-0.2 Å²	0 Å²	0.01 Å²
2-	-	0.15 Å²	0 Å²
3-	-	-	0.06 Å²

Refinement step

Cycle: LAST / Resolution: 2.4→33.77 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	5637	0	67	499	6203

Refine LS restraints

Refine-ID	Type	Dev ideal	Dev ideal target	Number
X-RAY DIFFRACTION	r_bond_refined_d	0.007	0.022	5870
X-RAY DIFFRACTION	r_bond_other_d
X-RAY DIFFRACTION	r_angle_refined_deg	0.873	1.98	7963
X-RAY DIFFRACTION	r_angle_other_deg
X-RAY DIFFRACTION	r_dihedral_angle_1_deg	4.401	5	730
X-RAY DIFFRACTION	r_dihedral_angle_2_deg	32.749	23.75	248
X-RAY DIFFRACTION	r_dihedral_angle_3_deg	13.949	15	996
X-RAY DIFFRACTION	r_dihedral_angle_4_deg	11.144	15	43
X-RAY DIFFRACTION	r_chiral_restr	0.055	0.2	888
X-RAY DIFFRACTION	r_gen_planes_refined	0.002	0.02	4423
X-RAY DIFFRACTION	r_gen_planes_other
X-RAY DIFFRACTION	r_nbd_refined	0.158	0.2	2946
X-RAY DIFFRACTION	r_nbd_other
X-RAY DIFFRACTION	r_nbtor_refined	0.291	0.2	4036
X-RAY DIFFRACTION	r_nbtor_other
X-RAY DIFFRACTION	r_xyhbond_nbd_refined	0.105	0.2	378
X-RAY DIFFRACTION	r_xyhbond_nbd_other
X-RAY DIFFRACTION	r_metal_ion_refined	0.044	0.2	2
X-RAY DIFFRACTION	r_metal_ion_other
X-RAY DIFFRACTION	r_symmetry_vdw_refined	0.143	0.2	66
X-RAY DIFFRACTION	r_symmetry_vdw_other
X-RAY DIFFRACTION	r_symmetry_hbond_refined	0.079	0.2	11
X-RAY DIFFRACTION	r_symmetry_hbond_other
X-RAY DIFFRACTION	r_symmetry_metal_ion_refined
X-RAY DIFFRACTION	r_symmetry_metal_ion_other
X-RAY DIFFRACTION	r_mcbond_it	1.197	2	3681
X-RAY DIFFRACTION	r_mcbond_other
X-RAY DIFFRACTION	r_mcangle_it	1.903	3	5984
X-RAY DIFFRACTION	r_scbond_it	1.492	2	2215
X-RAY DIFFRACTION	r_scangle_it	2.27	3	1979
X-RAY DIFFRACTION	r_rigid_bond_restr
X-RAY DIFFRACTION	r_sphericity_free
X-RAY DIFFRACTION	r_sphericity_bonded

LS refinement shell

Resolution: 2.4→2.462 Å / Total num. of bins used: 20

	R_factor	Num. reflection	% reflection
R_free	0.292	74	-
R_work	0.214	1338	-
obs	-	-	50.27 %

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	21.1869	-13.1396	6.678	25.7725	-10.8123	22.359	0.1273	-0.188	0.3556	0.3002	-0.3959	-0.5886	0.0315	1.4294	0.2686	0.0054	0.0644	0.0542	0.2172	0.0282	0.0619	13.008	-5.401	29.953
2	5.4681	1.0842	2.1515	10.8464	3.8518	1.95	0.3021	-0.2431	0.3262	-0.6081	-0.0338	0.0596	-0.0345	0.6806	-0.2683	0.1531	-0.0286	0.0747	0.2049	0.0037	0.0327	8.591	3.135	22.625
3	1.7837	-0.8539	0.1807	2.7629	-1.1794	2.6642	0.0177	-0.179	-0.2076	0.1722	-0.0355	0.0081	0.0822	0.4297	0.0179	0.0709	0.0244	0.0263	0.0836	0.0327	0.042	4.248	-5.258	28.73
4	3.4613	-3.4364	-1.5262	4.3102	-0.7833	6.5526	-0.0475	0.3206	-0.2504	0.2733	-0.074	-0.0938	0.3017	-0.0495	0.1216	0.0816	0.0094	0.0021	0.0584	-0.0557	0.0522	-0.774	-4.59	17.956
5	9.6306	-0.3983	-5.8278	10.7684	-8.7392	11.0272	0.1572	0.6117	-1.5213	-0.3191	0.1906	0.2794	0.6316	-0.5551	-0.3479	0.0657	0.0519	0.0293	0.0395	0.0004	0.1908	-3.961	-9.941	28.001
6	1.1255	-2.617	-1.0857	11.6579	-0.7173	2.9331	-0.1062	-0.2735	0.1055	0.2742	0.0954	-0.1025	-0.0969	0.1904	0.0108	0.0239	0.0094	-0.0219	0.0869	0.0011	0.04	-2.126	7.096	38.03
7	0.9871	-0.7599	0.1537	2.0555	1.611	2.0575	-0.1921	0.0313	-0.0186	-0.0019	0.1015	0.4565	0.1742	-0.344	0.0906	0.1102	0.0165	0.0245	0.0621	-0.0239	0.109	-9.708	6.084	33.254
8	2.6771	0.6697	0.6231	2.6546	-0.0584	0.716	0.0706	-0.1896	0.0717	0.3501	-0.1585	-0.0498	0.1348	-0.1427	0.0879	0.0938	0.0141	0.0178	0.0408	-0.0219	0.0291	-7.257	7.822	37.44
9	7.1943	1.68	7.2743	3.2228	3.158	12.3949	-0.0161	-0.1199	0.0256	0.0442	-0.355	0.6762	-0.0156	-0.773	0.3711	0.0044	0.089	-0.0469	0.166	-0.0951	0.248	-23.847	9.735	17.012
10	3.9068	-0.4152	0.5559	2.2378	1.6136	1.3545	-0.2913	0.2898	0.2772	-0.6012	0.0355	0.1241	-0.5528	0.0603	0.2558	0.1178	0.0301	-0.0056	0.0559	-0.0246	0.1165	-12.901	9.226	15.934
11	4.3731	-0.2028	5.6655	2.6421	2.0717	27.9263	0.0777	-0.1895	-0.3854	-0.1431	-0.3099	0.5417	0.2326	-1.1092	0.2322	-0.0301	0.0106	-0.0007	0.0996	-0.1012	0.1901	-23.339	0.103	17.844
12	1.0559	0.8171	3.4637	0.6408	2.7895	12.7604	-0.0111	-0.1196	-0.1784	0.2081	-0.0862	0.2239	0.558	0.0697	0.0973	0.0864	0.0207	0.0069	0.0918	-0.0487	0.1006	-14.298	-1.027	21.937
13	11.3749	8.8525	-4.0678	9.9465	-4.2407	8.0845	-0.4302	0.4523	-0.0207	-0.6525	0.0872	0.2674	0.0315	-0.4138	0.343	0.0867	0.0656	-0.0768	0.1477	-0.0973	-0.006	-10.726	1.41	5.809
14	10.6036	-4.8523	-3.0719	25.2736	3.8793	1.1554	0.16	0.0526	0.5084	2.2089	-0.7797	0.443	0.5151	0.7398	0.6197	0.3878	-0.1688	-0.1688	0.2658	-0.0685	0.3819	-0.411	15.12	11.224
15	1.0913	-0.1707	-1.2544	8.5583	0.4731	1.4509	-0.0625	0.1445	-0.4132	-0.3028	-0.2343	-0.7143	-0.0308	0.0604	0.2968	0.1282	0.0854	-0.0305	0.2501	-0.0303	0.0668	-0.084	-0.272	8.696
16	3.8852	0.2731	-1.4979	3.2776	1.5101	5.9236	0.0612	0.3371	0.1738	-0.4417	0.0934	-0.0325	-0.5375	0.0812	-0.1546	0.0513	-0.0092	0.0097	0.131	-0.006	0.0499	-2.618	4.245	6.984
17	4.0992	-0.1619	3.1264	5.7751	-3.1012	6.6284	-0.5001	0.4367	0.5856	-0.6752	0.2001	-0.2196	-1.0077	1.2066	0.3001	0.4371	-0.2071	-0.0205	0.2556	0.0793	0.1795	-9.4	45.651	29.141
18	5.6082	-4.9506	-1.4536	6.7677	-0.3682	1.5142	-0.132	0.4073	0.612	-0.1187	-0.1255	-0.394	-0.0334	0.1121	0.2575	0.1102	-0.047	-0.0061	0.0816	-0.026	0.0686	-10.897	36.494	30.816
19	8.1423	-6.7341	7.5513	10.6467	-6.6691	7.0386	0.4058	1.1471	0.5258	-0.7735	-0.6005	-0.4904	-0.0525	0.3877	0.1947	0.2985	-0.0512	0.0129	0.1364	0.0056	0.1047	-18.995	42.136	25.665
20	10.6372	1.5044	9.5105	0.3096	1.0827	9.2142	-0.3561	0.2191	0.3887	0.0059	0.3357	0.2607	-1.1341	0.1223	0.0204	0.4442	0.0419	-0.0654	-0.0327	-0.0517	0.2186	-18.583	49.34	35.382
21	15.7912	-3.8828	12.0149	1.3494	-3.3723	9.5845	0.0469	-0.6913	0.1721	-0.1122	-0.0039	0.0597	-0.1855	-0.5408	-0.043	0.1713	0.0158	-0.0381	0.057	-0.0934	0.1358	-19.683	41.551	38.334
22	2.2559	-1.2919	1.7955	3.684	0.5406	2.2652	-0.0342	0.1154	-0.0046	-0.0867	0	-0.1143	0.0469	0.1259	0.0342	0.0648	-0.0034	0.0067	0.0542	0.0091	0.0398	-16.953	26.974	26.908
23	1.8687	0.7372	3.85	1.0992	1.0285	8.2296	-0.0391	-0.1209	0.303	0.2809	0.0218	-0.2108	0.1795	-0.4317	0.0173	0.0747	0.0254	-0.0017	0.029	-0.0415	0.0898	-22.553	30.983	32.772
24	3.8193	2.5951	-0.3421	7.0128	4.3818	12.7388	-0.1316	0.2138	-0.1293	-0.2427	-0.1311	0.1958	-0.1	-0.1534	0.2627	0.0202	0.0515	0.0034	0.0343	-0.0018	0.0814	-17.02	23.363	23.793
25	2.6025	-3.2772	-5.447	5.3526	4.3959	17.0702	-0.1646	-0.5116	-0.0832	0.3442	0.2072	0.0384	0.7306	-0.1544	-0.0426	0.2203	-0.0353	0.02	0.1216	-0.004	0.0552	-15.502	18.06	53.63
26	1.899	-0.5089	-0.7744	1.0832	1.3353	6.8057	0.0181	-0.2056	-0.0088	0.3232	0.0518	-0.1003	0.2441	0.2994	-0.0699	0.1593	0.018	-0.0001	0.0372	-0.0347	0.0695	-12.091	25.265	50.077
27	3.5441	-0.241	-0.1041	0.5932	-1.6105	4.6538	0.0667	-0.1625	-0.2955	0.0777	0.0434	0.3128	0.2039	-0.5226	-0.1101	0.1045	-0.0588	0.0618	0.0905	-0.0475	0.1086	-23.649	24.381	47.404
28	0.0173	0.2253	0.1239	3.741	3.6246	5.8851	-0.1228	0.1084	0.3412	-0.0585	-0.0263	0.1754	-0.3082	-0.4812	0.1491	0.1175	-0.0134	0.0099	0.1549	-0.1046	0.0865	-18.972	34.45	51.633
29	40.0077	-9.7359	-25.7496	6.6011	10.8153	21.4629	1.0794	-0.5565	-0.6983	-0.827	1.1816	-0.9505	-0.3985	2.0977	-2.261	0.1879	0.1491	-0.0996	0.5913	-0.391	0.4182	4.3	31.595	52.624
30	2.4728	3.4835	2.6617	15.652	3.0933	2.905	0.1052	0.5304	-0.1032	-1.6388	0.7122	-1.3652	-0.4704	0.7499	-0.8174	0.1983	-0.0852	0.2356	0.2254	-0.1785	0.3398	-0.713	34.645	43.544
31	0.7673	1.9191	-1.3506	5.8333	-2.4074	3.2889	0.0573	-0.2498	0.3985	0.0419	-0.0591	-0.1901	-0.2079	-0.0167	0.0018	0.0895	0.0113	0.0077	0.0816	-0.1329	0.1223	-10.302	40.126	51.139
32	10.3558	-5.4245	-1.7775	6.8636	-3.7162	5.6745	-0.13	-0.8135	0.4294	0.0195	0.2348	-1.2738	-0.6375	1.5525	-0.1049	0.07	-0.0834	-0.0129	0.4162	-0.1958	0.3163	2.087	37.084	50.702
33	15.3684	2.2597	7.201	0.4993	-1.0669	30.4209	1.5996	-1.6232	-2.0506	0.5702	-0.5247	-1.1808	2.3351	-1.2423	-1.0749	0.5239	-0.2936	-0.3696	0.2176	0.2527	0.3062	-16.117	-26.584	-4.365
34	23.2326	0.0186	10.7428	4.9088	4.034	8.2685	1.1625	-0.4917	-1.4887	0.6671	-0.2696	-0.4145	1.3054	0.152	-0.8928	0.2114	0.0113	-0.1261	0.029	0.0614	0.2067	-8.497	-21.557	-5.891
35	6.3557	-8.8602	-6.9286	12.3516	9.6588	7.5531	-0.1063	-0.8844	0.316	1.5095	0.8773	0.3308	1.2285	-1.5107	-0.7711	0.4188	-0.1187	-0.036	0.8338	-0.0617	-0.0788	-15.797	-16.878	5.099
36	7.6058	-2.0794	3.2076	0.5977	-0.827	1.4382	0.8248	-2.9274	-0.8174	0.1017	-0.1302	0.3857	0.2701	-1.0777	-0.6947	0.0712	-0.2687	-0.0263	0.897	0.1909	0.11	-25.158	-19.102	-5.006
37	13.981	-0.3952	6.4441	0.5736	-1.082	8.8612	0.0754	-1.2542	0.2844	-0.1034	-0.0245	0.2136	0.1275	-0.6586	-0.0508	0.075	-0.0487	-0.0084	0.0908	-0.0227	0.034	-19.791	-15.955	-11.261
38	4.1017	3.5968	2.251	3.3804	2.9077	5.0865	0.0347	-0.1441	0.3859	0.1539	-0.155	-0.0662	0.553	-0.2933	0.1203	0.12	0.1033	-0.0418	0.2599	-0.1109	-0.0583	-6.496	-12.019	0.578
39	11.8471	-3.2065	1.426	8.0883	3.0864	11.9283	-0.3338	0.2474	0.846	-0.1196	0.1912	-0.9997	-0.0422	0.8661	0.1426	0.1054	0.0665	-0.0676	0.0652	-0.0525	0.1141	-1.094	-12.182	-5.815
40	10.0185	-1.4424	7.7608	5.2082	-2.2225	15.2082	-0.3972	-0.2821	0.6488	0.0562	0.181	-0.2781	0.0189	0.7547	0.2163	0.0706	0.043	-0.0427	0.0763	-0.1297	0.1605	0.454	-11.106	-1.856
41	0.1111	-0.3976	-1.0612	6.9774	0.7743	11.776	-0.1537	0.265	0.6558	0.0485	-0.4522	-1.4479	0.0285	1.355	0.6058	-0.0686	-0.0772	0.0374	0.3392	0.194	0.6588	-2.207	-4.926	-24.434
42	3.8895	-1.7265	1.6885	13.0849	-9.2501	13.3106	-0.2493	0.9832	0.2975	-1.1186	-0.354	-0.7916	0.5516	0.2684	0.6033	0.059	-0.1166	0.0906	0.1475	0.1028	0.1822	-9.74	-9.186	-25.529
43	11.4243	-0.8712	11.2163	5.9135	8.6862	32.6647	-0.2561	0.6787	1.4001	0.2751	0.1482	-1.1918	-1.1856	0.6449	0.1079	0.1465	-0.1333	-0.2244	-0.108	0.2443	0.6418	-10.462	2.147	-21.83
44	3.4046	0.0626	2.9929	1.5077	-1.0557	3.4497	-0.3489	0.1529	0.6319	0.1025	-0.1346	-0.1793	-0.3382	-0.0034	0.4834	0.0983	-0.0433	-0.0442	0.047	0.0449	0.1103	-15.468	-9.035	-21.897
45	22.5602	-2.1055	2.0378	12.6593	11.9303	21.0523	-0.6948	-0.6386	-0.5941	-0.4662	1.3827	-0.5885	-1.5158	-0.1505	-0.6878	0.3105	0.1582	0.0296	0.1784	0.2154	0.2196	-6.812	-27.927	-28.669
46	7.1781	1.5846	3.1302	1.6423	4.0042	9.8579	0.2163	-0.2194	-0.6697	-0.5228	0.0732	0.1179	0.9236	0.9314	-0.2895	0.1816	-0.0777	0.0089	0.2339	0.0732	0.2103	-19.833	-23.463	-20.951
47	6.4256	-0.8606	3.0603	2.5546	-2.2952	9.2065	-0.0199	0.2217	0.1835	-0.0851	0.1478	-0.1409	0.1789	0.0825	-0.1279	0.0973	-0.0105	-0.0234	-0.0238	-0.0321	0.0581	-20.116	-14.405	-20.866
48	5.2239	-2.7199	-3.9121	1.7947	0.2949	10.9451	0.2952	0.7345	-0.3358	0.1273	-0.17	0.4664	0.2994	-0.4098	-0.1252	0.1095	0.0153	-0.0129	0.1608	-0.0173	0.1358	-15.641	-24.806	-27.716

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	A	218 - 225
2	X-RAY DIFFRACTION	2	A	226 - 235
3	X-RAY DIFFRACTION	3	A	236 - 267
4	X-RAY DIFFRACTION	4	A	268 - 278
5	X-RAY DIFFRACTION	5	A	279 - 286
6	X-RAY DIFFRACTION	6	A	287 - 305
7	X-RAY DIFFRACTION	7	A	306 - 321
8	X-RAY DIFFRACTION	8	A	322 - 340
9	X-RAY DIFFRACTION	9	B	218 - 231
10	X-RAY DIFFRACTION	10	B	232 - 248
11	X-RAY DIFFRACTION	11	B	249 - 270
12	X-RAY DIFFRACTION	12	B	271 - 283
13	X-RAY DIFFRACTION	13	B	284 - 298
14	X-RAY DIFFRACTION	14	B	299 - 309
15	X-RAY DIFFRACTION	15	B	310 - 319
16	X-RAY DIFFRACTION	16	B	320 - 340
17	X-RAY DIFFRACTION	17	C	218 - 236
18	X-RAY DIFFRACTION	18	C	237 - 243
19	X-RAY DIFFRACTION	19	C	244 - 251
20	X-RAY DIFFRACTION	20	C	252 - 270
21	X-RAY DIFFRACTION	21	C	271 - 287
22	X-RAY DIFFRACTION	22	C	288 - 318
23	X-RAY DIFFRACTION	23	C	319 - 327
24	X-RAY DIFFRACTION	24	C	328 - 340
25	X-RAY DIFFRACTION	25	D	218 - 229
26	X-RAY DIFFRACTION	26	D	230 - 254
27	X-RAY DIFFRACTION	27	D	255 - 274
28	X-RAY DIFFRACTION	28	D	275 - 292
29	X-RAY DIFFRACTION	29	D	293 - 302
30	X-RAY DIFFRACTION	30	D	303 - 315
31	X-RAY DIFFRACTION	31	D	316 - 327
32	X-RAY DIFFRACTION	32	D	328 - 340
33	X-RAY DIFFRACTION	33	E	222 - 234
34	X-RAY DIFFRACTION	34	E	235 - 244
35	X-RAY DIFFRACTION	35	E	245 - 256
36	X-RAY DIFFRACTION	36	E	257 - 268
37	X-RAY DIFFRACTION	37	E	269 - 280
38	X-RAY DIFFRACTION	38	E	281 - 301
39	X-RAY DIFFRACTION	39	E	302 - 321
40	X-RAY DIFFRACTION	40	E	322 - 340
41	X-RAY DIFFRACTION	41	F	217 - 238
42	X-RAY DIFFRACTION	42	F	239 - 250
43	X-RAY DIFFRACTION	43	F	251 - 269
44	X-RAY DIFFRACTION	44	F	270 - 297
45	X-RAY DIFFRACTION	45	F	298 - 305
46	X-RAY DIFFRACTION	46	F	310 - 317
47	X-RAY DIFFRACTION	47	F	318 - 325
48	X-RAY DIFFRACTION	48	F	326 - 340

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