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- PDB-3l25: Crystal structure of Zaire Ebola VP35 interferon inhibitory domai... -

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Basic information

Entry
Database: PDB / ID: 3l25
TitleCrystal structure of Zaire Ebola VP35 interferon inhibitory domain bound to 8 bp dsRNA
Components
  • Polymerase cofactor VP35
  • RNA (5'-R(*CP*GP*CP*AP*UP*GP*CP*G)-3')
KeywordsRNA BINDING PROTEIN/RNA / RNA BINDING DOMAIN / COILED COIL / INTERFERON ANTIVIRAL SYSTEM EVASION / RNA REPLICATION / RNA BINDING PROTEIN / TRANSCRIPTION / Host cytoplasm / RNA-binding / Virion / RNA BINDING PROTEIN-RNA complex
Function / homology
Function and homology information


suppression by virus of host cytokine production / symbiont-mediated suppression of host defenses / symbiont-mediated suppression of host RNAi-mediated antiviral immune response / negative regulation of miRNA-mediated gene silencing / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF7 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IKBKE activity / suppression by virus of host type I interferon production / symbiont-mediated suppression of host antigen processing and presentation of peptide antigen via MHC class II / molecular sequestering activity / positive regulation of protein sumoylation ...suppression by virus of host cytokine production / symbiont-mediated suppression of host defenses / symbiont-mediated suppression of host RNAi-mediated antiviral immune response / negative regulation of miRNA-mediated gene silencing / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF7 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IKBKE activity / suppression by virus of host type I interferon production / symbiont-mediated suppression of host antigen processing and presentation of peptide antigen via MHC class II / molecular sequestering activity / positive regulation of protein sumoylation / : / viral transcription / viral genome replication / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / symbiont-mediated suppression of host toll-like receptor signaling pathway / viral nucleocapsid / host cell cytoplasm / negative regulation of gene expression / RNA binding
Similarity search - Function
Filoviridae VP35, C-terminal inhibitory domain, beta-sheet subdomain / Filoviridae VP35, C-terminal inhibitory domain, helical subdomain / Filoviruses VP35 interferon inhibitory domain, beta-sheet subdomain / Filoviridae VP35 protein / Filoviruses VP35 interferon inhibitory domain / Filoviruses VP35 interferon inhibitory domain, helical subdomain / Filoviridae VP35 / Filoviruses VP35 interferon inhibitory domain profile. / Seminal Fluid Protein PDC-109 (Domain B) / Helicase, Ruva Protein; domain 3 ...Filoviridae VP35, C-terminal inhibitory domain, beta-sheet subdomain / Filoviridae VP35, C-terminal inhibitory domain, helical subdomain / Filoviruses VP35 interferon inhibitory domain, beta-sheet subdomain / Filoviridae VP35 protein / Filoviruses VP35 interferon inhibitory domain / Filoviruses VP35 interferon inhibitory domain, helical subdomain / Filoviridae VP35 / Filoviruses VP35 interferon inhibitory domain profile. / Seminal Fluid Protein PDC-109 (Domain B) / Helicase, Ruva Protein; domain 3 / Ribbon / Orthogonal Bundle / Mainly Beta / Mainly Alpha
Similarity search - Domain/homology
FORMIC ACID / RNA / Polymerase cofactor VP35
Similarity search - Component
Biological speciesZaire ebolavirus
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å
AuthorsLeung, D.W. / Prins, K.C. / Borek, D.M. / Farahbakhsh, M. / Tufariello, J.M. / Ramanan, P. / Nix, J.C. / Helgeson, L.A. / Otwinowski, Z. / Honzatko, R.B. ...Leung, D.W. / Prins, K.C. / Borek, D.M. / Farahbakhsh, M. / Tufariello, J.M. / Ramanan, P. / Nix, J.C. / Helgeson, L.A. / Otwinowski, Z. / Honzatko, R.B. / Basler, C.F. / Amarasinghe, G.K.
Citation
#1: Journal: To be Published
Title: Preliminary X-ray studies of the Ebola VP35 IFN inhibitory domain bound to double stranded RNA
Authors: Leung, D.W. / Prins, K.C. / Borek, D.M. / Farahbakhsh, M. / Tufariello, J.M. / Ramanan, P. / Nix, J.C. / Helgeson, L.A. / Otwinowski, Z. / Honzatko, R.B. / Basler, C.F. / Amarasinghe, G.K.
History
DepositionDec 14, 2009Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 26, 2010Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Version format compliance
Revision 1.2Sep 6, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Polymerase cofactor VP35
B: Polymerase cofactor VP35
C: RNA (5'-R(*CP*GP*CP*AP*UP*GP*CP*G)-3')
D: Polymerase cofactor VP35
E: Polymerase cofactor VP35
F: RNA (5'-R(*CP*GP*CP*AP*UP*GP*CP*G)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)63,47244
Polymers61,9256
Non-polymers1,54738
Water6,035335
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)78.947, 84.571, 89.316
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

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Protein / RNA chain , 2 types, 6 molecules ABDECF

#1: Protein
Polymerase cofactor VP35


Mass: 14210.523 Da / Num. of mol.: 4 / Fragment: Zaire Ebola VP35 interferon inhibitory domain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Zaire ebolavirus / Strain: Mayinga-76 / Gene: VP35 / Plasmid: MODIFIED PET15B / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q05127
#2: RNA chain RNA (5'-R(*CP*GP*CP*AP*UP*GP*CP*G)-3')


Mass: 2541.577 Da / Num. of mol.: 2 / Source method: obtained synthetically

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Non-polymers , 6 types, 373 molecules

#3: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Mg
#4: Chemical
ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Cl
#5: Chemical
ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Na
#6: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H8O3
#7: Chemical...
ChemComp-FMT / FORMIC ACID / Formic acid


Mass: 46.025 Da / Num. of mol.: 24 / Source method: obtained synthetically / Formula: CH2O2
#8: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 335 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.41 Å3/Da / Density % sol: 48.91 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.2
Details: 3M SODIUM FORMATE, 0.1M SODIUM CITRATE, 5% GLYCEROL, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Components of the solutions
IDNameCrystal-IDSol-ID
1SODIUM FORMATE11
2SODIUM CITRATE11
3GLYCEROL11
4SODIUM FORMATE12
5SODIUM CITRATE12
6GLYCEROL12

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.979 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Mar 20, 2009
RadiationMonochromator: DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 2→50 Å / Num. all: 41135 / Num. obs: 41135 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.8 % / Rmerge(I) obs: 0.121 / Net I/σ(I): 15.9
Reflection shellResolution: 2→2.03 Å / Redundancy: 4.7 % / Rmerge(I) obs: 0.758 / Mean I/σ(I) obs: 2.2 / % possible all: 100

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Processing

Software
NameVersionClassification
HKL-3000data collection
PHASERphasing
REFMAC5.3.0037refinement
HKL-3000data reduction
SCALEPACKdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 3FKE

3fke


Resolution: 2→44.68 Å / Cor.coef. Fo:Fc: 0.958 / Cor.coef. Fo:Fc free: 0.939 / SU B: 5.526 / SU ML: 0.1 / Isotropic thermal model: ISOTROPIC / Cross valid method: THROUGHOUT / ESU R: 0.164 / ESU R Free: 0.145 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.20744 2038 5 %RANDOM
Rwork0.16938 ---
all0.17129 39012 --
obs0.17129 39012 99.7 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 17.138 Å2
Baniso -1Baniso -2Baniso -3
1-0 Å20 Å20 Å2
2--0 Å20 Å2
3---0 Å2
Refinement stepCycle: LAST / Resolution: 2→44.68 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3862 336 94 335 4627
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0110.0224381
X-RAY DIFFRACTIONr_bond_other_d0.0020.028
X-RAY DIFFRACTIONr_angle_refined_deg1.2262.0785976
X-RAY DIFFRACTIONr_angle_other_deg1.241316
X-RAY DIFFRACTIONr_dihedral_angle_1_deg4.7735498
X-RAY DIFFRACTIONr_dihedral_angle_2_deg32.19223.952167
X-RAY DIFFRACTIONr_dihedral_angle_3_deg13.73215691
X-RAY DIFFRACTIONr_dihedral_angle_4_deg18.881528
X-RAY DIFFRACTIONr_chiral_restr0.0780.2681
X-RAY DIFFRACTIONr_gen_planes_refined0.0050.023164
X-RAY DIFFRACTIONr_nbd_refined0.1910.22060
X-RAY DIFFRACTIONr_nbd_other0.2040.210
X-RAY DIFFRACTIONr_nbtor_refined0.2970.23002
X-RAY DIFFRACTIONr_nbtor_other0.0690.22
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.1360.2316
X-RAY DIFFRACTIONr_metal_ion_refined0.0970.219
X-RAY DIFFRACTIONr_symmetry_vdw_refined0.2110.277
X-RAY DIFFRACTIONr_symmetry_vdw_other0.2990.21
X-RAY DIFFRACTIONr_symmetry_hbond_refined0.1420.226
X-RAY DIFFRACTIONr_mcbond_it0.4711.52548
X-RAY DIFFRACTIONr_mcangle_it0.87524068
X-RAY DIFFRACTIONr_scbond_it1.57731932
X-RAY DIFFRACTIONr_scangle_it2.4744.51908
LS refinement shellResolution: 2→2.051 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.243 143 -
Rwork0.211 2773 -
obs--96.84 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
120.319508.110713.3804026.2624-0.1007-0.5573-1.1163-0.46860.6084-1.26850.34781.4551-0.50770.04680.1132-0.01980.2212-0.0950.28326.158-2.016-39.65
25.4447-1.51831.61343.1903-0.23273.7059-0.00240.305-0.1243-0.16980.11-0.38860.09950.5463-0.1075-0.06690.00730.01140.0069-0.0211-0.0545-2.3274.052-38.8
313.80582.6941-4.21835.5183-1.28634.15920.0187-0.8647-0.8520.5358-0.1384-0.62470.33850.8440.11970.0640.0376-0.07420.08360.01790.0968-0.287-0.504-28.063
417.10967.27982.05399.03143.2581.20450.1395-1.0701-0.05270.6771-0.1483-0.2117-0.16320.3110.00880.06080.0003-0.02590.04220.0207-0.0179-1.377.992-26.86
56.71931.3255.16835.0441-0.86188.9854-0.34410.03650.0957-0.13750.0511-0.2561-0.70290.07140.2929-0.0588-0.04240.0817-0.0163-0.00350.0012-3.2915.944-42.833
66.2869-9.5018-5.027918.71995.55834.97640.26760.2160.2685-0.3243-0.35720.3316-0.08630.16190.0897-0.0751-0.0552-0.0286-0.0409-0.0081-0.0639-16.33510.559-43.908
71.4049-1.50870.63113.3496-1.58324.6088-0.32470.07270.43590.30880.0302-0.2786-0.69290.15420.29460.048-0.0715-0.0277-0.03560.0158-0.027-7.16421.526-35.133
83.81720.9071-1.04123.4575-0.48833.6124-0.2340.32750.1194-0.30030.18850.1644-0.0335-0.11720.0455-0.0599-0.0893-0.031-0.02620.021-0.0385-7.35619.73-41.861
921.40043.749720.155610.6072-4.309325.1618-0.3927-1.12582.27281.2860.1712-0.953-0.80430.15750.22160.2856-0.0129-0.01010.127-0.26150.3097-16.37330.569-9.148
107.50870.58482.58123.66091.46125.1536-0.0484-0.30240.44370.3676-0.0740.1793-0.2146-0.23830.1223-0.02960.01090.0145-0.056-0.0312-0.0391-23.18722.681-15.68
114.7346-0.08013.1923.5313-1.35166.4535-0.1014-0.33270.220.1224-0.0037-0.1442-0.47530.05140.1052-0.0175-0.0286-0.0256-0.053-0.0306-0.0248-12.99323.802-19.154
125.6782-2.29470.36638.05982.19184.63280.0836-0.26060.00420.2617-0.0749-0.25590.15550.3277-0.0087-0.0967-0.0401-0.0036-0.05220.019-0.0883-11.88616.316-19.074
1317.29640.4122-2.17165.4662-1.78754.1244-0.18270.1199-0.56080.4920.05590.2798-0.2742-0.18330.12680.10950.0281-0.0483-0.06840.0105-0.0961-23.889.755-8.295
1414.723-5.90961.311311.3734-4.41391.79580.0646-0.1246-0.0420.8410.01260.3056-1.07210.2732-0.0771-0.01430.03040.0352-0.1356-0.06050.0473-34.95114.917-13.765
157.9367-3.03963.12497.4564-1.69845.07770.21690.0629-0.2074-0.1433-0.03560.3977-0.0705-0.1844-0.1812-0.04930.00830.0041-0.1001-0.0118-0.1182-26.4974.14-16.334
166.53180.4317-1.04153.92670.28243.50730.0668-0.0215-0.16540.1233-0.08410.1292-0.032-0.25940.0172-0.02820.0175-0.0289-0.1087-0.0035-0.0779-24.9716.37-12.062
1710.73415.5758-1.277117.6388-1.60999.39920.3954-0.4119-1.51510.3845-0.1853-0.92960.7318-0.2455-0.21020.05720.0211-0.0788-0.00040.02440.1932-20.353-23.804-25.115
183.2049-0.3025-0.70766.86821.94323.4030.0814-0.2095-0.0090.1740.0096-0.3580.1660.2906-0.0909-0.0992-0.023-0.0234-0.08520.0085-0.0928-23.35-11.258-26.198
197.25335.94792.268735.79321.62132.43160.1001-0.7724-0.49860.7928-0.10950.23610.5411-0.07740.0094-0.065-0.01080.0133-0.01810.05650.0017-30.514-22.225-25.81
206.70972.8735-1.78679.9609-2.6592.9099-0.26030.344-0.7381-0.58680.0084-0.69280.4118-0.03380.25180.00190.01630.0029-0.0216-0.04610.0027-23.059-19.931-35.763
2110.73279.88110.98524.0499-4.74234.5887-0.22210.718-0.257-0.78260.2534-0.24190.4765-0.1606-0.03120.0129-0.01650.001-0.0157-0.0135-0.0522-31.523-19.172-37.439
225.42942.52431.57343.95392.88394.71180.2088-0.4470.0520.2359-0.3660.28170.1978-0.41760.1573-0.0066-0.01810.0123-0.02220.044-0.0422-36.906-12.16-19.229
237.16711.93192.7030.53880.55362.71890.2589-0.23170.00990.1547-0.09970.0979-0.0004-0.3558-0.1593-0.00740.01180.0121-0.01240.0015-0.0011-39.883-5.148-25.9
245.3534-0.70171.56054.46560.275413.5640.0511-0.38880.10570.4447-0.07050.23630.0832-0.74160.0195-0.1032-0.06060.0379-0.05350.0006-0.022-40.493-10.607-24.863
2511.1812-4.6344-9.814329.5786-5.966112.25490.15911.4506-0.3229-1.8714-0.34440.87650.9519-1.26680.18530.2336-0.1329-0.30060.37650.16790.4183-50.3560.116-55.928
262.75713.09311.593913.02050.61671.0652-0.06370.10030.3316-0.4430.35421.1132-0.1361-0.6921-0.2906-0.04360.0369-0.02310.08080.10550.0624-44.878.435-47.286
273.5188-1.6569-0.427110.26356.49469.77180.21990.53110.2756-0.61180.1368-0.0051-0.5022-0.2826-0.3566-0.09410.0190.021-0.04830.0584-0.0629-37.516.355-49.609
285.68741.7245-1.32567.7777-0.39159.6217-0.23710.8997-0.0212-0.72730.40830.33640.2826-0.6389-0.17110.0285-0.1121-0.08650.11770.03570.0275-43.72-4.567-52.362
295.2338-0.6978-1.97144.0376-1.62051.7187-0.15080.1916-0.08710.1250.17060.06580.1975-0.2555-0.0199-0.1018-0.0356-0.0201-0.05330.0027-0.0777-39.506-3.688-42.402
304.27590.194-1.90498.5747-0.31637.16660.02330.40440.3409-0.40280.2091-0.2826-0.3555-0.2725-0.2324-0.15560.011-0.00610.0541-0.0033-0.0428-29.2159.516-55.27
315.52441.2355-0.62021.88832.20553.4788-0.05770.07610.02370.09770.03010.0110.0538-0.11070.0276-0.10580.0252-0.0005-0.0709-0.0023-0.097-25.2224.141-48.469
325.20712.1419-2.1148.8427-5.986415.55530.15610.13380.12880.01780.0222-0.1814-0.61430.1617-0.1783-0.10420.01060.0038-0.0689-0.0128-0.0587-24.10610.279-55.4
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A218 - 224
2X-RAY DIFFRACTION2A225 - 254
3X-RAY DIFFRACTION3A255 - 274
4X-RAY DIFFRACTION4A275 - 285
5X-RAY DIFFRACTION5A286 - 304
6X-RAY DIFFRACTION6A305 - 309
7X-RAY DIFFRACTION7A310 - 321
8X-RAY DIFFRACTION8A322 - 340
9X-RAY DIFFRACTION9B218 - 223
10X-RAY DIFFRACTION10B224 - 250
11X-RAY DIFFRACTION11B251 - 276
12X-RAY DIFFRACTION12B277 - 286
13X-RAY DIFFRACTION13B287 - 301
14X-RAY DIFFRACTION14B302 - 307
15X-RAY DIFFRACTION15B308 - 318
16X-RAY DIFFRACTION16B319 - 340
17X-RAY DIFFRACTION17D218 - 227
18X-RAY DIFFRACTION18D228 - 243
19X-RAY DIFFRACTION19D244 - 251
20X-RAY DIFFRACTION20D252 - 277
21X-RAY DIFFRACTION21D278 - 287
22X-RAY DIFFRACTION22D288 - 305
23X-RAY DIFFRACTION23D306 - 317
24X-RAY DIFFRACTION24D318 - 340
25X-RAY DIFFRACTION25E218 - 223
26X-RAY DIFFRACTION26E224 - 237
27X-RAY DIFFRACTION27E238 - 245
28X-RAY DIFFRACTION28E246 - 264
29X-RAY DIFFRACTION29E265 - 287
30X-RAY DIFFRACTION30E288 - 306
31X-RAY DIFFRACTION31E307 - 326
32X-RAY DIFFRACTION32E327 - 340

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