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基本情報
登録情報 | データベース: PDB / ID: 3jbr | ||||||
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タイトル | Cryo-EM structure of the rabbit voltage-gated calcium channel Cav1.1 complex at 4.2 angstrom | ||||||
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![]() | MEMBRANE PROTEIN / voltage-gated calcium channel | ||||||
機能・相同性 | ![]() voltage-gated calcium channel activity involved in regulation of presynaptic cytosolic calcium levels / Phase 2 - plateau phase / Phase 0 - rapid depolarisation / Presynaptic depolarization and calcium channel opening / Regulation of insulin secretion / positive regulation of calcium ion transmembrane transport via high voltage-gated calcium channel / positive regulation of high voltage-gated calcium channel activity / membrane depolarization during atrial cardiac muscle cell action potential / positive regulation of muscle contraction / membrane depolarization during AV node cell action potential ...voltage-gated calcium channel activity involved in regulation of presynaptic cytosolic calcium levels / Phase 2 - plateau phase / Phase 0 - rapid depolarisation / Presynaptic depolarization and calcium channel opening / Regulation of insulin secretion / positive regulation of calcium ion transmembrane transport via high voltage-gated calcium channel / positive regulation of high voltage-gated calcium channel activity / membrane depolarization during atrial cardiac muscle cell action potential / positive regulation of muscle contraction / membrane depolarization during AV node cell action potential / regulation of presynaptic cytosolic calcium ion concentration / high voltage-gated calcium channel activity / L-type voltage-gated calcium channel complex / photoreceptor ribbon synapse / positive regulation of calcium ion transport / calcium ion import / regulation of calcium ion transmembrane transport via high voltage-gated calcium channel / voltage-gated calcium channel complex / neuromuscular junction development / cellular response to caffeine / calcium ion import across plasma membrane / regulation of heart rate by cardiac conduction / calcium channel regulator activity / regulation of ryanodine-sensitive calcium-release channel activity / voltage-gated calcium channel activity / muscle contraction / release of sequestered calcium ion into cytosol / T-tubule / visual perception / protein localization to plasma membrane / phosphoprotein binding / calcium ion transmembrane transport / sarcolemma / calcium ion transport / actin filament binding / presynapse / chemical synaptic transmission / transmembrane transporter binding / calmodulin binding / protein domain specific binding / protein kinase binding / identical protein binding / metal ion binding / plasma membrane 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() ![]() ![]() | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.2 Å | ||||||
![]() | Wu, J.P. / Yan, Z. / Yan, N. | ||||||
![]() | ![]() タイトル: Structure of the voltage-gated calcium channel Cav1.1 complex. 著者: Jianping Wu / Zhen Yan / Zhangqiang Li / Chuangye Yan / Shan Lu / Mengqiu Dong / Nieng Yan / ![]() 要旨: The voltage-gated calcium channel Ca(v)1.1 is engaged in the excitation-contraction coupling of skeletal muscles. The Ca(v)1.1 complex consists of the pore-forming subunit α1 and auxiliary subunits ...The voltage-gated calcium channel Ca(v)1.1 is engaged in the excitation-contraction coupling of skeletal muscles. The Ca(v)1.1 complex consists of the pore-forming subunit α1 and auxiliary subunits α2δ, β, and γ. We report the structure of the rabbit Ca(v)1.1 complex determined by single-particle cryo-electron microscopy. The four homologous repeats of the α1 subunit are arranged clockwise in the extracellular view. The γ subunit, whose structure resembles claudins, interacts with the voltage-sensing domain of repeat IV (VSD(IV)), whereas the cytosolic β subunit is located adjacent to VSD(II) of α1. The α2 subunit interacts with the extracellular loops of repeats I to III through its VWA and Cache1 domains. The structure reveals the architecture of a prototypical eukaryotic Ca(v) channel and provides a framework for understanding the function and disease mechanisms of Ca(v) and Na(v) channels. | ||||||
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構造の表示
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構造ビューア | 分子: ![]() ![]() |
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PDBx/mmCIF形式 | ![]() | 448 KB | 表示 | ![]() |
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PDB形式 | ![]() | 335.4 KB | 表示 | ![]() |
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-関連構造データ
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集合体
登録構造単位 | ![]()
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要素
-Voltage-dependent L-type calcium channel subunit ... , 2種, 2分子 AB
#1: タンパク質 | 分子量: 208655.859 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() ![]() |
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#2: タンパク質 | 分子量: 40300.004 Da / 分子数: 1 / 由来タイプ: 組換発現 詳細: Chain B is for a subunit that is docked by a homolog crystal structure (PDB code: 1T0J) 由来: (組換発現) ![]() ![]() ![]() ![]() |
-Voltage-dependent calcium channel ... , 2種, 2分子 EF
#3: タンパク質 | 分子量: 25082.254 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() ![]() |
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#4: タンパク質 | 分子量: 116775.609 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() ![]() |
-糖 , 2種, 17分子 ![](data/chem/img/NAG.gif)
![](data/chem/img/BMA.gif)
![](data/chem/img/BMA.gif)
#6: 糖 | ChemComp-NAG / #7: 糖 | |
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-非ポリマー , 1種, 1分子 ![](data/chem/img/CA.gif)
#5: 化合物 | ChemComp-CA / |
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-詳細
配列の詳細 | RESIDUES 1396-1520 OF ENTITY 1 (CHAIN A), AND RESIDUES 40-55/662-955 OF ENTITY 4 (CHAIN F) ARE ...RESIDUES 1396-1520 OF ENTITY 1 (CHAIN A), AND RESIDUES 40-55/662-955 OF ENTITY 4 (CHAIN F) ARE MODELED AS UNK SINCE THE EM DENSITY IS NOT GOOD ENOUGH TO ASSIGN EXACTLY. THE REAL SEQUENCE OF RESIDUES 1396-1520 OF ENTITY 1 (CHAIN A) SHOULD BE (PHHLDEFKAI |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 | 名称: a membrane protein cryo-sample / タイプ: COMPLEX / 別称: Cav1.1 |
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分子量 | 値: 0.45 MDa / 実験値: NO |
緩衝液 | 名称: 20mM MOPS, 200mM NaCl, 0.5mM CaCl2,0.1% digitonin / pH: 7.4 / 詳細: 20mM MOPS, 200mM NaCl, 0.5mM CaCl2,0.1% digitonin |
試料 | 濃度: 0.1 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
試料支持 | 詳細: carbon coated grid |
急速凍結 | 装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE / 湿度: 100 % / 手法: Blot for 2 seconds before plunging |
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電子顕微鏡撮影
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS / 日付: 2015年2月2日 |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 3.3 nm / 最小 デフォーカス(公称値): 2 nm / Cs: 2.7 mm / カメラ長: 0 mm |
試料ホルダ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER 傾斜角・最大: 0 ° / 傾斜角・最小: 0 ° |
撮影 | 電子線照射量: 50 e/Å2 / フィルム・検出器のモデル: GATAN K2 (4k x 4k) |
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解析
EMソフトウェア |
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CTF補正 | 詳細: each micrograph | ||||||||||||
対称性 | 点対称性: C1 (非対称) | ||||||||||||
3次元再構成 | 解像度: 4.2 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 353372 詳細: (Single particle details: The particles were selected using RELION.) (Single particle--Applied symmetry: C1) 対称性のタイプ: POINT | ||||||||||||
原子モデル構築 | プロトコル: RIGID BODY FIT / 空間: REAL 詳細: REFINEMENT PROTOCOL--rigid body DETAILS--The fitting is mostly refer to a related entry | ||||||||||||
原子モデル構築 | PDB-ID: 1T0J | ||||||||||||
精密化ステップ | サイクル: LAST
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