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Open data
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Basic information
Entry | Database: PDB / ID: 3iqc | ||||||
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Title | Crystal structure of FliS from H. pylori | ||||||
![]() | Flagellar protein | ||||||
![]() | CHAPERONE / flagella / Flagellum | ||||||
Function / homology | ![]() bacterial-type flagellum assembly / bacterial-type flagellum-dependent cell motility / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Lam, W.W.L. / Ling, T.K.W. / Woo, E.J. / Au, S.W.N. | ||||||
![]() | ![]() Title: Molecular interaction of flagellar export chaperone FliS and cochaperone HP1076 in Helicobacter pylori Authors: Lam, W.W.L. / Woo, E.J. / Kotaka, M. / Tam, W.K. / Leung, Y.C. / Ling, T.K.W. / Au, S.W.N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 101.5 KB | Display | ![]() |
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PDB format | ![]() | 79.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 438.1 KB | Display | ![]() |
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Full document | ![]() | 441.4 KB | Display | |
Data in XML | ![]() | 10 KB | Display | |
Data in CIF | ![]() | 13 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3k1hC ![]() 3k1iC ![]() 1orjS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Ens-ID: 1 / Beg auth comp-ID: LYS / Beg label comp-ID: LYS / End auth comp-ID: ILE / End label comp-ID: ILE / Refine code: 5 / Auth seq-ID: 22 - 120 / Label seq-ID: 27 - 125
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Components
#1: Protein | Mass: 14969.931 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.65 Å3/Da / Density % sol: 53.55 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 7.7 Details: 0.1M Hepes, 0.2M NaCl, 25% PEG3350, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Date: Aug 28, 2006 |
Radiation | Monochromator: varimax / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→42.68 Å / Num. obs: 8726 / % possible obs: 97.6 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 5 % / Rmerge(I) obs: 0.038 / Net I/σ(I): 24.4 |
Reflection shell | Resolution: 2.7→2.85 Å / Redundancy: 5.1 % / Rmerge(I) obs: 0.499 / Mean I/σ(I) obs: 3 / Num. unique all: 1270 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1ORJ Resolution: 2.7→30.14 Å / Cor.coef. Fo:Fc: 0.938 / Cor.coef. Fo:Fc free: 0.949 / SU B: 32.078 / SU ML: 0.3 / Cross valid method: THROUGHOUT / ESU R: 0.516 / ESU R Free: 0.365 Stereochemistry target values: MAXIMUM LIKELIHOOD WITH PHASES Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 72.863 Å2
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Refinement step | Cycle: LAST / Resolution: 2.7→30.14 Å
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Refine LS restraints |
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Refine LS restraints NCS | Dom-ID: 1 / Auth asym-ID: A / Ens-ID: 1 / Refine-ID: X-RAY DIFFRACTION
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LS refinement shell | Resolution: 2.7→2.77 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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