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Open data
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Basic information
Entry | Database: PDB / ID: 3k1h | ||||||
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Title | Crystal structure of HP1076 from H.pylori | ||||||
![]() | Putative uncharacterized protein | ||||||
![]() | CHAPERONE / FliS interacting protein / hypothetical protein | ||||||
Function / homology | Flagellar FLiS export co-chaperone, HP1076 / Flagellar FLiS export co-chaperone, HP1076 / FLiS export co-chaperone, HP1076 superfamily / Flagellar FLiS export co-chaperone, HP1076 / Arylsulfatase, C-terminal domain / 2-Layer Sandwich / Alpha Beta / Uncharacterized protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lam, W.W.L. / Ling, T.K.W. / Woo, E.J. / Au, S.W.N. | ||||||
![]() | ![]() Title: Molecular interaction of flagellar export chaperone FliS and cochaperone HP1076 in Helicobacter pylori Authors: Lam, W.W.L. / Woo, E.J. / Kotaka, M. / Tam, W.K. / Leung, Y.C. / Ling, T.K.W. / Au, S.W.N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 59.5 KB | Display | ![]() |
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PDB format | ![]() | 43.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 418.1 KB | Display | ![]() |
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Full document | ![]() | 418.1 KB | Display | |
Data in XML | ![]() | 7.2 KB | Display | |
Data in CIF | ![]() | 9.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 17989.537 Da / Num. of mol.: 1 / Fragment: Residues 21-171 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 46.34 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.1M Bis-Tris, 27% PEG 3350, 0.2M Lithium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Oct 26, 2008 |
Radiation | Monochromator: double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97958 Å / Relative weight: 1 |
Reflection | Resolution: 1.74→50 Å / Num. obs: 16258 / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 7.4 % / Rmerge(I) obs: 0.056 / Net I/σ(I): 45.8 |
Reflection shell | Resolution: 1.74→1.8 Å / Redundancy: 6.1 % / Rmerge(I) obs: 0.443 / Num. unique all: 1456 / % possible all: 90.4 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: MAXIMUM LIKELIHOOD WITH PHASES Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.381 Å2
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Refinement step | Cycle: LAST / Resolution: 1.74→26.06 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.737→1.782 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Origin x: 11.568 Å / Origin y: 14.602 Å / Origin z: -18.096 Å
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