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- PDB-3gmv: Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3gmv | ||||||
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Title | Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Apo Form | ||||||
![]() | Beta-lactamase inhibitory protein BLIP-I | ||||||
![]() | PROTEIN BINDING / 2-layer alpha/beta sandwich | ||||||
Function / homology | BLIP domain / Beta-lactamase-inhibitor protein BLIP / Beta-lactamase-inhibitor protein BLIP domain superfamily / Beta-lactamase inhibitor (BLIP) / Nuclear Transport Factor 2; Chain: A, / Roll / Alpha Beta / Beta-lactamase inhibitory protein BLIP-I precusor![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Lim, D.C. / Gretes, M. / Strynadka, N.C.J. | ||||||
![]() | ![]() Title: Insights into positive and negative requirements for protein-protein interactions by crystallographic analysis of the beta-lactamase inhibitory proteins BLIP, BLIP-I, and BLP. Authors: Gretes, M. / Lim, D.C. / de Castro, L. / Jensen, S.E. / Kang, S.G. / Lee, K.J. / Strynadka, N.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 44.9 KB | Display | ![]() |
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PDB format | ![]() | 34.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 431.3 KB | Display | ![]() |
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Full document | ![]() | 437.1 KB | Display | |
Data in XML | ![]() | 10.9 KB | Display | |
Data in CIF | ![]() | 15.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17218.963 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-TAM / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.77 Å3/Da / Density % sol: 55.58 % |
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Crystal grow | Temperature: 294 K / Method: hanging drop vapor batch Details: 20 mg/ml protein in 50 mM tris, pH 8.0, 50 mM NaCl mixed 1:1 with well solution 30% PEG 4000, 0.3 M lithium chloride, 0.1 M TrisCl, pH 8.5, hanging drop vapor batch, temperature 294K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Oct 13, 2000 |
Radiation | Monochromator: DOUBLE CRYSTAL SILICON (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→25 Å / Num. obs: 17522 / % possible obs: 91 % / Rsym value: 0.041 / Net I/σ(I): 28.336 |
Reflection shell | Resolution: 1.75→1.81 Å / Redundancy: 5.7 % / Mean I/σ(I) obs: 11.6 / Rsym value: 0.094 / % possible all: 68.7 |
-Phasing
Phasing | Method: ![]() |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 14.96 Å2
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Refinement step | Cycle: LAST / Resolution: 1.8→20.49 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.846 Å / Total num. of bins used: 20
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