分子量: 29397.609 Da / 分子数: 2 / 断片: residues 429-544, 667-806 / 変異: I473H, K525E, I780L, Q784K / 由来タイプ: 組換発現 詳細: The I442H K494E I749L and Q753K mutations were created intentionally. The 1st r esidue is a vector encoded affinity tag fragment. Residues 398-513 and 636-775 a re coupled by a synthetic GT ...詳細: The I442H K494E I749L and Q753K mutations were created intentionally. The 1st r esidue is a vector encoded affinity tag fragment. Residues 398-513 and 636-775 a re coupled by a synthetic GT peptide. The numbering is for the mature protein af ter cleavage of the 31 AA signal peptide. 由来: (組換発現) Rattus norvegicus (ドブネズミ) / 遺伝子: GriK2 / プラスミド: pET22B modified / 発現宿主: Escherichia coli (大腸菌) / 株 (発現宿主): OrigamiB (DE3) / 参照: UniProt: P42260
解像度: 1.37→1.42 Å / 冗長度: 1.6 % / Rmerge(I) obs: 0.241 / Mean I/σ(I) obs: 2.9 / % possible all: 89.3
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解析
ソフトウェア
名称
バージョン
分類
HKL-2000
データ収集
PHENIX
(phenix.refine)
精密化
HKL-2000
データ削減
HKL-2000
データスケーリング
精密化
構造決定の手法: フーリエ合成 / 解像度: 1.371→28.687 Å / SU ML: 0.16 / Isotropic thermal model: ISOTROPIC AND TLS / 交差検証法: THROUGHOUT / σ(F): 0 / σ(I): 0 / 立体化学のターゲット値: ML 詳細: Refinement was started with Refmac_5.2. The final rounds of refinement were performed with phenix and included occupancy refinement for ions, and for residues with alternative conformations.
Rfactor
反射数
%反射
Selection details
Rfree
0.176
5438
4.99 %
RANDOM
Rwork
0.1493
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all
0.1506
109022
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obs
0.1506
109022
95.93 %
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溶媒の処理
減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL / Bsol: 43.238 Å2 / ksol: 0.413 e/Å3