ジャーナル: EMBO J / 年: 2009 タイトル: The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria. 著者: Anastasia A Aksyuk / Petr G Leiman / Lidia P Kurochkina / Mikhail M Shneider / Victor A Kostyuchenko / Vadim V Mesyanzhinov / Michael G Rossmann / 要旨: The contractile tail of bacteriophage T4 is a molecular machine that facilitates very high viral infection efficiency. Its major component is a tail sheath, which contracts during infection to less ...The contractile tail of bacteriophage T4 is a molecular machine that facilitates very high viral infection efficiency. Its major component is a tail sheath, which contracts during infection to less than half of its initial length. The sheath consists of 138 copies of the tail sheath protein, gene product (gp) 18, which surrounds the central non-contractile tail tube. The contraction of the sheath drives the tail tube through the outer membrane, creating a channel for the viral genome delivery. A crystal structure of about three quarters of gp18 has been determined and was fitted into cryo-electron microscopy reconstructions of the tail sheath before and after contraction. It was shown that during contraction, gp18 subunits slide over each other with no apparent change in their structure.
A: Tail sheath protein Gp18 B: Tail sheath protein Gp18 C: Tail sheath protein Gp18 D: Tail sheath protein Gp18 E: Tail sheath protein Gp18 F: Tail sheath protein Gp18
分子量: 54645.965 Da / 分子数: 6 / 断片: deletion mutant gp18M: UNP Residues 1-510 / 変異: R510P / 由来タイプ: 組換発現 詳細: six gp18M fitted into one disk of the tail sheath in the contracted conformation 由来: (組換発現) Enterobacteria phage T4 (ファージ) 遺伝子: 18, GB AAA32541 / プラスミド: pET22 / 発現宿主: Escherichia coli (大腸菌) / 株 (発現宿主): BL21(DE3) / 参照: UniProt: P13332
配列の詳細
AUTHORS STATE THAT THE SEQUENCE COMPLETELY AGREES WITH THE GENBANK ENTRY AAA32541, PUBMED REPORT ...AUTHORS STATE THAT THE SEQUENCE COMPLETELY AGREES WITH THE GENBANK ENTRY AAA32541, PUBMED REPORT 2964531. THE SEQUENCE DIFFERENCES MAY REFLECT THE SEQUENCE VARIATION BETWEEN THE VIRUS STRAINS.