Journal: Cell / Year: 2004 Title: Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host. Authors: Petr G Leiman / Paul R Chipman / Victor A Kostyuchenko / Vadim V Mesyanzhinov / Michael G Rossmann / Abstract: The contractile tail of bacteriophage T4 undergoes major structural transitions when the virus attaches to the host cell surface. The baseplate at the distal end of the tail changes from a hexagonal ...The contractile tail of bacteriophage T4 undergoes major structural transitions when the virus attaches to the host cell surface. The baseplate at the distal end of the tail changes from a hexagonal to a star shape. This causes the sheath around the tail tube to contract and the tail tube to protrude from the baseplate and pierce the outer cell membrane and the cell wall before reaching the inner cell membrane for subsequent viral DNA injection. Analogously, the T4 tail can be contracted by treatment with 3 M urea. The structure of the T4 contracted tail, including the head-tail joining region, has been determined by cryo-electron microscopy to 17 A resolution. This 1200 A-long, 20 MDa structure has been interpreted in terms of multiple copies of its approximately 20 component proteins. A comparison with the metastable hexagonal baseplate of the mature virus shows that the baseplate proteins move as rigid bodies relative to each other during the structural change.
History
Deposition
Jul 19, 2004
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Header (metadata) release
Jul 19, 2004
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Map release
Sep 7, 2004
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Update
May 26, 2011
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Current status
May 26, 2011
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Name: Enterobacteria phage T4 / type: virus / ID: 1 / Name.synonym: phage T4 / Details: treated with 3 M urea / NCBI-ID: 10665 / Sci species name: Enterobacteria phage T4 / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No / Syn species name: phage T4
Host (natural)
Organism: Escherichia coli (E. coli) / synonym: BACTERIA(EUBACTERIA)
Molecular weight
Experimental: 220 MDa / Theoretical: 220 MDa
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Experimental details
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Structure determination
Method
cryo EM
Processing
single particle reconstruction
Aggregation state
particle
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Sample preparation
Concentration
5 mg/mL
Buffer
pH: 7.5 / Details: H2O
Grid
Details: 200 mesh cupper grid
Vitrification
Cryogen name: ETHANE / Chamber temperature: 100 K
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Electron microscopy
Microscope
FEI/PHILIPS CM300FEG/T
Temperature
Average: 100 K
Specialist optics
Energy filter - Name: FEI
Date
Jan 6, 2002
Image recording
Category: FILM / Film or detector model: KODAK SO-163 FILM / Digitization - Scanner: ZEISS SCAI / Digitization - Sampling interval: 4.51104 µm / Number real images: 100 / Average electron dose: 20 e/Å2 / Bits/pixel: 8
Tilt angle min
0
Tilt angle max
0
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
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