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- PDB-3e9l: Crystal Structure of Human Prp8, Residues 1755-2016 -

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Basic information

Entry
Database: PDB / ID: 3e9l
TitleCrystal Structure of Human Prp8, Residues 1755-2016
ComponentsPre-mRNA-processing-splicing factor 8
KeywordsRNA BINDING PROTEIN / SPLICING / nucleotidyl transfer / Disease mutation / mRNA processing / mRNA splicing / Nucleus / Phosphoprotein / Retinitis pigmentosa / RNA-binding / Sensory transduction / Spliceosome / Vision
Function / homology
Function and homology information


RNA splicing, via transesterification reactions / U2-type catalytic step 1 spliceosome / U2-type precatalytic spliceosome / U2-type catalytic step 2 spliceosome / K63-linked polyubiquitin modification-dependent protein binding / mRNA Splicing - Minor Pathway / spliceosomal tri-snRNP complex assembly / U5 snRNP / U5 snRNA binding / U2 snRNA binding ...RNA splicing, via transesterification reactions / U2-type catalytic step 1 spliceosome / U2-type precatalytic spliceosome / U2-type catalytic step 2 spliceosome / K63-linked polyubiquitin modification-dependent protein binding / mRNA Splicing - Minor Pathway / spliceosomal tri-snRNP complex assembly / U5 snRNP / U5 snRNA binding / U2 snRNA binding / pre-mRNA intronic binding / U6 snRNA binding / U4/U6 x U5 tri-snRNP complex / U1 snRNA binding / isopeptidase activity / catalytic step 2 spliceosome / mRNA Splicing - Major Pathway / RNA splicing / mRNA splicing, via spliceosome / mRNA processing / cellular response to tumor necrosis factor / cellular response to lipopolysaccharide / nuclear speck / RNA binding / membrane / nucleoplasm / nucleus
Similarity search - Function
Prp8 RNase H domain, fingers region / Prp8 RNase H domain, palm region / Acyl-CoA Binding Protein / PROCT domain / Prp8 RNase domain IV, fingers region / PROCT (NUC072) domain / Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding / PROCN domain / PRO8NT domain / Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain superfamily ...Prp8 RNase H domain, fingers region / Prp8 RNase H domain, palm region / Acyl-CoA Binding Protein / PROCT domain / Prp8 RNase domain IV, fingers region / PROCT (NUC072) domain / Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding / PROCN domain / PRO8NT domain / Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain superfamily / Prp8 RNase domain IV, palm region / Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding / RNA recognition motif, spliceosomal PrP8 / PRP8 domain IV core / U6-snRNA interacting domain of PrP8 / PRP8 domain IV core / PRO8NT (NUC069), PrP8 N-terminal domain / PROCN (NUC071) domain / RNA recognition motif of the spliceosomal PrP8 / U5-snRNA binding site 2 of PrP8 / Pre-mRNA-processing-splicing factor 8 / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / JAB/MPN domain / JAB1/MPN/MOV34 metalloenzyme domain / MPN domain / MPN domain profile. / Nucleotidyltransferase; domain 5 / Ribonuclease H-like superfamily / Up-down Bundle / 2-Layer Sandwich / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
Pre-mRNA-processing-splicing factor 8
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.95 Å
AuthorsPena, V. / Rozov, A. / Wahl, M.C.
CitationJournal: Embo J. / Year: 2008
Title: Structure and function of an RNase H domain at the heart of the spliceosome.
Authors: Pena, V. / Rozov, A. / Fabrizio, P. / Luhrmann, R. / Wahl, M.C.
History
DepositionAug 22, 2008Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 7, 2008Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Advisory / Version format compliance

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Pre-mRNA-processing-splicing factor 8
hetero molecules


Theoretical massNumber of molelcules
Total (without water)29,5944
Polymers29,5121
Non-polymers813
Water5,675315
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
γ
α
β
Length a, b, c (Å)83.426, 83.426, 96.263
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number154
Space group name H-MP3221

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Components

#1: Protein Pre-mRNA-processing-splicing factor 8 / Splicing factor Prp8 / PRP8 homolog / 220 kDa U5 snRNP-specific protein / p220


Mass: 29512.238 Da / Num. of mol.: 1 / Fragment: endonuclease domain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: PRPF8, PRPC8 / Production host: Escherichia coli (E. coli) / References: UniProt: Q6P2Q9
#2: Chemical ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Na
#3: Chemical ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 315 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.28 Å3/Da / Density % sol: 62.46 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5
Details: pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: BESSY / Beamline: 14.2 / Wavelength: 0.91841 Å
DetectorType: MAR CCD 165 mm / Detector: CCD / Date: Feb 28, 2008
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.91841 Å / Relative weight: 1
ReflectionResolution: 1.95→50 Å / Num. all: 28624 / Num. obs: 28624 / % possible obs: 99 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2
Reflection shellResolution: 1.95→2.02 Å / % possible all: 99.9

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Processing

Software
NameVersionClassification
REFMAC5.2.0019refinement
DENZOdata reduction
SCALEPACKdata scaling
SHELXSphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.95→30 Å / Cor.coef. Fo:Fc: 0.952 / Cor.coef. Fo:Fc free: 0.93 / SU B: 6.441 / SU ML: 0.103 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / σ(F): 2 / ESU R: 0.153 / ESU R Free: 0.149 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.24804 181 5 %RANDOM
Rwork0.205 ---
all0.22 3623 --
obs0.20503 28264 98.7 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK
Displacement parametersBiso mean: 38.754 Å2
Baniso -1Baniso -2Baniso -3
1-0.92 Å20.46 Å20 Å2
2--0.92 Å20 Å2
3----1.38 Å2
Refinement stepCycle: LAST / Resolution: 1.95→30 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2083 0 3 315 2401
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0090.0222178
X-RAY DIFFRACTIONr_angle_refined_deg1.1181.9642964
X-RAY DIFFRACTIONr_dihedral_angle_1_deg5.6175266
X-RAY DIFFRACTIONr_dihedral_angle_2_deg38.95624.94795
X-RAY DIFFRACTIONr_dihedral_angle_3_deg14.43115406
X-RAY DIFFRACTIONr_dihedral_angle_4_deg14.546159
X-RAY DIFFRACTIONr_chiral_restr0.0780.2344
X-RAY DIFFRACTIONr_gen_planes_refined0.0040.021605
X-RAY DIFFRACTIONr_nbd_refined0.1960.21052
X-RAY DIFFRACTIONr_nbtor_refined0.3080.21537
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.1360.2219
X-RAY DIFFRACTIONr_metal_ion_refined0.1370.27
X-RAY DIFFRACTIONr_symmetry_vdw_refined0.1560.232
X-RAY DIFFRACTIONr_symmetry_hbond_refined0.1620.214
X-RAY DIFFRACTIONr_mcbond_it0.4921.51312
X-RAY DIFFRACTIONr_mcangle_it0.9122154
X-RAY DIFFRACTIONr_scbond_it1.4493902
X-RAY DIFFRACTIONr_scangle_it2.4144.5809
LS refinement shellResolution: 1.95→2.003 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.321 91 -
Rwork0.222 1936 -
obs-2828 98.79 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
11.0757-0.1661-0.00711.76290.75732.467-0.01090.06230.0791-0.28320.1438-0.0033-0.23250.0909-0.1328-0.0862-0.016-0.0084-0.09170.027-0.137231.30219.1878.123
20.5335-0.1882-0.22753.8894-0.26311.5664-0.082-0.12050.05190.28610.13250.3810.0472-0.2622-0.0505-0.13660.040.0451-0.0190.0259-0.0820.15614.87226.718
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
1X-RAY DIFFRACTION1AA1761 - 19212 - 162
2X-RAY DIFFRACTION2AA1922 - 2014163 - 255

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