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- PDB-3dw5: Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23S rRN... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3dw5 | ||||||
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Title | Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23S rRNA, U2656-OCH3 modified | ||||||
![]() | Sarcin/Ricin Domain from E. Coli 23 S rRNA | ||||||
![]() | RNA / Sarcin Ricin Loop | ||||||
Function / homology | RNA / RNA (> 10)![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Olieric, V. / Rieder, U. / Lang, K. / Serganov, A. / Schulze-Briese, C. / Micura, R. / Dumas, P. / Ennifar, E. | ||||||
![]() | ![]() Title: A fast selenium derivatization strategy for crystallization and phasing of RNA structures. Authors: Olieric, V. / Rieder, U. / Lang, K. / Serganov, A. / Schulze-Briese, C. / Micura, R. / Dumas, P. / Ennifar, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 44.4 KB | Display | ![]() |
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PDB format | ![]() | 32.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 393.8 KB | Display | ![]() |
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Full document | ![]() | 394 KB | Display | |
Data in XML | ![]() | 5.3 KB | Display | |
Data in CIF | ![]() | 7.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3dvzC ![]() 3dw4C ![]() 3dw6C ![]() 3dw7C ![]() 1q9aS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: RNA chain | Mass: 8758.282 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 35.25 % | ||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 3.2 M (NH4)2SO4, 50 mM K-MOPS pH 7.0, 10 mM MgCl2, 10 mM MnCl2, vapor diffusion, hanging drop, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 90 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Apr 22, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.85 Å / Relative weight: 1 |
Reflection | Highest resolution: 0.96 Å / Num. obs: 39656 / % possible obs: 99.8 % / Redundancy: 11.9 % / Biso Wilson estimate: 12.625 Å2 / Rsym value: 0.032 / Net I/σ(I): 34.5 |
Reflection shell | Resolution: 0.96→0.97 Å / Mean I/σ(I) obs: 3.1 / Num. measured obs: 9571 / Num. unique obs: 1123 / Rsym value: 0.634 / % possible all: 92.9 |
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Processing
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Refinement | Starting model: 1q9a Resolution: 0.96→27.557 Å / Occupancy max: 1 / Occupancy min: 0.25 / SU ML: 0.09 / σ(F): 2 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 39.753 Å2 / ksol: 0.436 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 35.27 Å2 / Biso mean: 14.204 Å2 / Biso min: 7.01 Å2
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Refinement step | Cycle: LAST / Resolution: 0.96→27.557 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 14
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