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- PDB-3cfj: Crystal structure of catalytic elimination antibody 34E4, orthorh... -

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Basic information

Entry
Database: PDB / ID: 3cfj
TitleCrystal structure of catalytic elimination antibody 34E4, orthorhombic crystal form
Components
  • CATALYTIC ANTIBODY FAB 34E4 HEAVY CHAIN fusion
  • CATALYTIC ANTIBODY FAB 34E4 LIGHT CHAIN fusion
KeywordsIMMUNE SYSTEM / IMMUNOGLOBULIN / CATALYTIC ANTIBODY / CHIMERIC FAB / APO FORM / PROTON TRANSFER / CONFORMATIONAL CHANGE / Immunoglobulin domain / Immunoglobulin V region
Function / homology
Function and homology information


Immunoglobulin V-Type / Immunoglobulin V-set domain / Immunoglobulin V-set domain / Immunoglobulin subtype / Immunoglobulin / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain ...Immunoglobulin V-Type / Immunoglobulin V-set domain / Immunoglobulin V-set domain / Immunoglobulin subtype / Immunoglobulin / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins / Immunoglobulin-like fold / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Uncharacterized protein / Uncharacterized protein / Uncharacterized protein
Similarity search - Component
Biological speciesMus musculus (house mouse)
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å
AuthorsDebler, E.W. / Wilson, I.A.
CitationJournal: J.Biol.Chem. / Year: 2008
Title: Conformational isomerism can limit antibody catalysis.
Authors: Debler, E.W. / Muller, R. / Hilvert, D. / Wilson, I.A.
History
DepositionMar 4, 2008Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 15, 2008Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Advisory / Version format compliance
Revision 1.2Jul 19, 2017Group: Database references / Source and taxonomy / Structure summary
Category: entity / entity_src_gen ...entity / entity_src_gen / struct_ref / struct_ref_seq / struct_ref_seq_dif
Item: _entity.details / _entity.pdbx_description

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
L: CATALYTIC ANTIBODY FAB 34E4 LIGHT CHAIN fusion
H: CATALYTIC ANTIBODY FAB 34E4 HEAVY CHAIN fusion
A: CATALYTIC ANTIBODY FAB 34E4 LIGHT CHAIN fusion
B: CATALYTIC ANTIBODY FAB 34E4 HEAVY CHAIN fusion
C: CATALYTIC ANTIBODY FAB 34E4 LIGHT CHAIN fusion
D: CATALYTIC ANTIBODY FAB 34E4 HEAVY CHAIN fusion
E: CATALYTIC ANTIBODY FAB 34E4 LIGHT CHAIN fusion
F: CATALYTIC ANTIBODY FAB 34E4 HEAVY CHAIN fusion
hetero molecules


Theoretical massNumber of molelcules
Total (without water)192,40714
Polymers191,8438
Non-polymers5646
Water9,332518
1
L: CATALYTIC ANTIBODY FAB 34E4 LIGHT CHAIN fusion
H: CATALYTIC ANTIBODY FAB 34E4 HEAVY CHAIN fusion
hetero molecules


Theoretical massNumber of molelcules
Total (without water)48,1494
Polymers47,9612
Non-polymers1882
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4550 Å2
ΔGint28.4 kcal/mol
Surface area20320 Å2
MethodPISA
2
A: CATALYTIC ANTIBODY FAB 34E4 LIGHT CHAIN fusion
B: CATALYTIC ANTIBODY FAB 34E4 HEAVY CHAIN fusion
hetero molecules


Theoretical massNumber of molelcules
Total (without water)48,0533
Polymers47,9612
Non-polymers921
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3980 Å2
ΔGint17.5 kcal/mol
Surface area20560 Å2
MethodPISA
3
C: CATALYTIC ANTIBODY FAB 34E4 LIGHT CHAIN fusion
D: CATALYTIC ANTIBODY FAB 34E4 HEAVY CHAIN fusion
hetero molecules


Theoretical massNumber of molelcules
Total (without water)48,2455
Polymers47,9612
Non-polymers2843
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4260 Å2
ΔGint19.6 kcal/mol
Surface area20500 Å2
MethodPISA
4
E: CATALYTIC ANTIBODY FAB 34E4 LIGHT CHAIN fusion
F: CATALYTIC ANTIBODY FAB 34E4 HEAVY CHAIN fusion


Theoretical massNumber of molelcules
Total (without water)47,9612
Polymers47,9612
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3800 Å2
ΔGint17.4 kcal/mol
Surface area20350 Å2
MethodPISA
Unit cell
γ
α
β
Length a, b, c (Å)81.137, 114.471, 212.131
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11L
21A
31C
41E
12L
22A
32C
42E
13L
23A
33C
43E
14L
24A
34C
44E
15H
25B
35D
45F
16L
26A
36C
46E
17H
27B
37D
47F
18H
28B
38D
48F
19H
29B
39D
49F
110H
210D
111B
211F

NCS domain segments:

Component-ID: 1 / Refine code: 3

Dom-IDEns-IDBeg label comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11LEUSERLA2 - 252 - 24
21LEUSERAC2 - 252 - 24
31LEUSERCE2 - 252 - 24
41LEUSEREG2 - 252 - 24
12SERTHRLA27 - 8026 - 82
22SERTHRAC27 - 8026 - 82
32SERTHRCE27 - 8026 - 82
42SERTHREG27 - 8026 - 82
13LEULYSLA96 - 10798 - 109
23LEULYSAC96 - 10798 - 109
33LEULYSCE96 - 10798 - 109
43LEULYSEG96 - 10798 - 109
14ARGARGLA108 - 211110 - 213
24ARGARGAC108 - 211110 - 213
34ARGARGCE108 - 211110 - 213
44ARGARGEG108 - 211110 - 213
15VALMETHB2 - 602 - 61
25VALMETBD2 - 602 - 61
35VALMETDF2 - 602 - 61
45VALMETFH2 - 602 - 61
16ASPCYSLA82 - 8884 - 90
26ASPCYSAC82 - 8884 - 90
36ASPCYSCE82 - 8884 - 90
46ASPCYSEG82 - 8884 - 90
17PHEASPHB67 - 9668 - 100
27PHEASPBD67 - 9668 - 100
37PHEASPDF67 - 9668 - 100
47PHEASPFH67 - 9668 - 100
18TYRSERHB102 - 113112 - 123
28TYRSERBD102 - 113112 - 123
38TYRSERDF102 - 113112 - 123
48TYRSERFH102 - 113112 - 123
19ALAPROHB114 - 227124 - 223
29ALAPROBD114 - 227124 - 223
39ALAPRODF114 - 227124 - 223
49ALAPROFH114 - 227124 - 223
110PROLYSHB61 - 6662 - 67
210PROLYSDF61 - 6662 - 67
111PROLYSBD61 - 6662 - 67
211PROLYSFH61 - 6662 - 67

NCS ensembles :
ID
1
2
3
4
5
6
7
8
9
10
11

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Components

#1: Antibody
CATALYTIC ANTIBODY FAB 34E4 LIGHT CHAIN fusion


Mass: 23258.826 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Details: FUSION PROTEIN OF THE VARIABLE DOMAIN from mouse (RESIDUES 1-107) AND THE CONSTANT DOMAIN from human (UNP RESIDUES 131-239)
Source: (gene. exp.) Mus musculus (house mouse), (gene. exp.) Homo sapiens (human)
Plasmid: P4XH-M13 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): TOPP2 / References: UniProt: Q8TCD0
#2: Antibody
CATALYTIC ANTIBODY FAB 34E4 HEAVY CHAIN fusion


Mass: 24701.814 Da / Num. of mol.: 4 / Mutation: H108S
Source method: isolated from a genetically manipulated source
Details: FUSION PROTEIN OF THE VARIABLE DOMAIN from mouse(RESIDUES 1-113) AND THE CONSTANT DOMAIN from human (UNP RESIDUES 133-246)
Source: (gene. exp.) Mus musculus (house mouse), (gene. exp.) Homo sapiens (human)
Plasmid: P4XH-M13 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): TOPP2 / References: UniProt: A8K008, UniProt: Q6N089*PLUS
#3: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C3H8O3
#4: Chemical ChemComp-SO4 / SULFATE ION / Sulfate


Mass: 96.063 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: SO4
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 518 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.59 Å3/Da / Density % sol: 52.48 %
Crystal growpH: 4.6
Details: 30% MPEG 2000, 0.2M (NH4)2SO4, 0.1M ACETATE, PH 4.6, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K, pH 4.60

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 8.2.1 / Wavelength: 1.03319
DetectorType: ADSC QUANTUM 210 / Detector: CCD / Date: Jan 28, 2005 / Details: MONOCHROMATOR
RadiationMonochromator: DOUBLE CRYSTAL, SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.03319 Å / Relative weight: 1
ReflectionResolution: 2.6→50 Å / Num. obs: 63551 / % possible obs: 99.7 % / Observed criterion σ(I): 0 / Redundancy: 4.1 % / Biso Wilson estimate: 51.4 Å2 / Rsym value: 0.108 / Net I/σ(I): 11.9
Reflection shellResolution: 2.6→2.69 Å / Redundancy: 4.1 % / Mean I/σ(I) obs: 2 / Rsym value: 0.628 / % possible all: 99.9

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Processing

Software
NameVersionClassification
PHASERphasing
REFMAC5.2.0019refinement
HKL-2000data reduction
HKL-2000data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 1Y0L
Resolution: 2.6→48.34 Å / Cor.coef. Fo:Fc: 0.943 / Cor.coef. Fo:Fc free: 0.905 / SU B: 22.59 / SU ML: 0.233 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 1.22 / ESU R Free: 0.328 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.253 3094 5 %RANDOM
Rwork0.199 ---
obs0.202 58339 99.8 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 38.44 Å2
Baniso -1Baniso -2Baniso -3
1--0.55 Å20 Å20 Å2
2--1.79 Å20 Å2
3----1.24 Å2
Refinement stepCycle: LAST / Resolution: 2.6→48.34 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13377 0 33 518 13928
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0130.02213731
X-RAY DIFFRACTIONr_bond_other_d
X-RAY DIFFRACTIONr_angle_refined_deg1.421.95918690
X-RAY DIFFRACTIONr_angle_other_deg
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.31151748
X-RAY DIFFRACTIONr_dihedral_angle_2_deg34.3124.23539
X-RAY DIFFRACTIONr_dihedral_angle_3_deg16.889152211
X-RAY DIFFRACTIONr_dihedral_angle_4_deg18.9331560
X-RAY DIFFRACTIONr_chiral_restr0.0930.22116
X-RAY DIFFRACTIONr_gen_planes_refined0.0050.0210304
X-RAY DIFFRACTIONr_gen_planes_other
X-RAY DIFFRACTIONr_nbd_refined0.2150.25286
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined0.3060.29182
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.1480.2636
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined0.2580.296
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined0.2060.219
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it0.9371.58915
X-RAY DIFFRACTIONr_mcbond_other
X-RAY DIFFRACTIONr_mcangle_it1.069214107
X-RAY DIFFRACTIONr_scbond_it2.09335524
X-RAY DIFFRACTIONr_scangle_it3.1114.54583
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
Refine LS restraints NCS

Refine-ID: X-RAY DIFFRACTION

Ens-IDDom-IDAuth asym-IDNumberTypeRms dev position (Å)Weight position
11L92tight positional0.050.05
12A92tight positional0.040.05
13C92tight positional0.040.05
14E92tight positional0.050.05
21L220tight positional0.040.05
22A220tight positional0.040.05
23C220tight positional0.040.05
24E220tight positional0.040.05
31L48tight positional0.050.05
32A48tight positional0.040.05
33C48tight positional0.050.05
34E48tight positional0.040.05
41L416tight positional0.060.05
42A416tight positional0.040.05
43C416tight positional0.040.05
44E416tight positional0.050.05
51H240tight positional0.050.05
52B240tight positional0.040.05
53D240tight positional0.040.05
54F240tight positional0.050.05
61L36tight positional0.050.05
62A36tight positional0.030.05
63C36tight positional0.040.05
64E36tight positional0.040.05
71H132tight positional0.040.05
72B132tight positional0.040.05
73D132tight positional0.040.05
74F132tight positional0.040.05
81H48tight positional0.040.05
82B48tight positional0.050.05
83D48tight positional0.050.05
84F48tight positional0.040.05
91H400tight positional0.050.05
92B400tight positional0.050.05
93D400tight positional0.040.05
94F400tight positional0.050.05
101H24tight positional0.030.05
111B24tight positional0.050.05
11L73loose positional0.395
12A73loose positional0.215
13C73loose positional0.275
14E73loose positional0.325
21L183loose positional0.55
22A183loose positional0.45
23C183loose positional0.445
24E183loose positional0.515
31L40loose positional0.425
32A40loose positional0.345
33C40loose positional0.345
34E40loose positional0.45
41L391loose positional0.555
42A391loose positional0.495
43C391loose positional0.495
44E391loose positional0.575
51H225loose positional0.535
52B225loose positional0.675
53D225loose positional0.415
54F225loose positional0.575
61L36loose positional0.685
62A36loose positional0.645
63C36loose positional0.335
64E36loose positional0.335
71H143loose positional0.465
72B143loose positional0.515
73D143loose positional0.485
74F143loose positional0.735
81H41loose positional0.55
82B41loose positional0.555
83D41loose positional0.535
84F41loose positional0.665
91H321loose positional0.455
92B321loose positional0.465
93D321loose positional0.475
94F321loose positional0.495
101H23loose positional0.375
111B23loose positional0.795
11L92tight thermal0.160.5
12A92tight thermal0.110.5
13C92tight thermal0.120.5
14E92tight thermal0.120.5
21L220tight thermal0.130.5
22A220tight thermal0.120.5
23C220tight thermal0.110.5
24E220tight thermal0.10.5
31L48tight thermal0.150.5
32A48tight thermal0.130.5
33C48tight thermal0.110.5
34E48tight thermal0.110.5
41L416tight thermal0.120.5
42A416tight thermal0.110.5
43C416tight thermal0.10.5
44E416tight thermal0.090.5
51H240tight thermal0.10.5
52B240tight thermal0.090.5
53D240tight thermal0.110.5
54F240tight thermal0.090.5
61L36tight thermal0.080.5
62A36tight thermal0.090.5
63C36tight thermal0.140.5
64E36tight thermal0.10.5
71H132tight thermal0.10.5
72B132tight thermal0.110.5
73D132tight thermal0.110.5
74F132tight thermal0.10.5
81H48tight thermal0.10.5
82B48tight thermal0.10.5
83D48tight thermal0.10.5
84F48tight thermal0.090.5
91H400tight thermal0.110.5
92B400tight thermal0.110.5
93D400tight thermal0.090.5
94F400tight thermal0.090.5
101H24tight thermal0.140.5
111B24tight thermal0.070.5
11L73loose thermal1.8510
12A73loose thermal1.0410
13C73loose thermal0.9410
14E73loose thermal1.7610
21L183loose thermal1.5610
22A183loose thermal1.1310
23C183loose thermal1.3510
24E183loose thermal1.4410
31L40loose thermal1.7710
32A40loose thermal1.7610
33C40loose thermal2.2710
34E40loose thermal1.4110
41L391loose thermal1.5910
42A391loose thermal1.410
43C391loose thermal1.2110
44E391loose thermal1.3810
51H225loose thermal1.9110
52B225loose thermal1.7410
53D225loose thermal1.3410
54F225loose thermal1.8410
61L36loose thermal1.0810
62A36loose thermal1.6810
63C36loose thermal210
64E36loose thermal2.0410
71H143loose thermal2.0710
72B143loose thermal1.8410
73D143loose thermal1.5910
74F143loose thermal1.5110
81H41loose thermal1.2310
82B41loose thermal1.1810
83D41loose thermal1.3610
84F41loose thermal2.2810
91H321loose thermal1.2710
92B321loose thermal1.0210
93D321loose thermal1.1410
94F321loose thermal1.3510
101H23loose thermal0.9110
111B23loose thermal0.610
LS refinement shellResolution: 2.6→2.67 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.358 247 -
Rwork0.295 4225 -
obs--99.98 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
14.9082-0.2480.52412.4644-0.00823.1423-0.10830.14270.17460.0230.0002-0.1436-0.08320.0460.1081-0.1329-0.00710.0009-0.27550.0204-0.204733.4882-11.52794.7275
23.28890.6615-0.34661.88410.91714.6836-0.22980.2298-0.2477-0.3520.09180.05270.1360.02920.138-0.0962-0.02330.0205-0.27390.0039-0.173913.9472-26.8867-21.5006
33.4645-0.304-0.09574.24861.26634.03850.00690.09950.28910.1161-0.0432-0.15090.02590.05230.0363-0.2106-0.02880.0178-0.1680.0591-0.078346.1715-3.6536-10.3702
46.9873-0.6259-1.50351.3530.37122.8653-0.02830.40350.3441-0.12490.08160.0867-0.1314-0.1071-0.0532-0.0696-0.02840.017-0.21140.0127-0.110119.6173-13.1866-26.7923
53.62391.10970.08393.17340.20555.48980.0407-0.2872-0.12670.0399-0.1701-0.01990.1469-0.13340.1294-0.1701-0.0085-0.0001-0.1534-0.0089-0.221424.0456-17.332125.3977
63.42481.22273.29792.24180.12248.37630.0534-0.2118-0.1052-0.05940.0312-0.3009-0.04910.4235-0.0846-0.1987-0.05150.02080.0289-0.0354-0.098945.526-4.299251.0891
73.3440.4241.33033.2914-0.1075.16160.18310.2698-0.21480.2106-0.2394-0.04680.0950.12160.0563-0.0336-0.17690.02210.0831-0.0326-0.147611.8322-24.878440.7677
82.46361.17480.2987.2289-0.26533.73960.1875-0.34430.41810.1772-0.26030.1263-0.1249-0.06520.0728-0.2049-0.05570.00360.0691-0.0619-0.096130.7549-3.288356.3287
94.05210.8654-0.44393.8470.57934.4073-0.06810.04210.0490.0768-0.20160.15130.0265-0.00870.2697-0.08710.01230.021-0.2034-0.0128-0.17966.354411.31893.9707
104.37290.7739-0.00611.9798-0.85086.9049-0.09060.13050.3002-0.39370.001-0.184-0.5280.09440.08960.0305-0.02540.0238-0.27610.0105-0.02627.708326.4497-20.8775
113.55780.5974-0.19514.4083-1.2564.2820.0509-0.00720.10150.0472-0.14340.3092-0.0759-0.30340.0925-0.18610.0433-0.0048-0.0921-0.0572-0.0844-4.84333.2489-12.1706
129.8486-1.30351.43931.5536-0.32493.4009-0.13510.5633-0.4375-0.18770.088-0.2437-0.11740.15890.04710.0451-0.03710.0255-0.2237-0.01330.016122.946512.5424-26.3948
133.37980.96870.17633.51990.34665.33960.093-0.1899-0.1650.0721-0.3450.08640.0846-0.21980.252-0.0635-0.03460.0144-0.0095-0.0204-0.159926.6044-17.1801-80.742
142.95250.97562.51982.6211-0.10696.0096-0.0112-0.2228-0.0124-0.21610.0951-0.2557-0.0620.4202-0.0839-0.182-0.04630.02840.1276-0.0233-0.103949.234-3.4249-56.0486
153.48460.23171.16832.53020.42784.6245-0.10850.4523-0.36890.2991-0.23030.48650.3804-0.23770.33890.132-0.19420.12640.2327-0.11420.063215.5281-25.2102-64.5471
161.81541.102-0.1646.5755-0.79753.47090.0039-0.16360.31530.0889-0.00830.2335-0.27030.1150.0044-0.1847-0.0593-0.02070.08010.0225-0.042134.8636-3.5149-49.8813
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
1X-RAY DIFFRACTION1LA1 - 1071 - 109
2X-RAY DIFFRACTION2LA108 - 213110 - 215
3X-RAY DIFFRACTION3HB1 - 1131 - 123
4X-RAY DIFFRACTION4HB114 - 228124 - 224
5X-RAY DIFFRACTION5AC1 - 1071 - 109
6X-RAY DIFFRACTION6AC108 - 213110 - 215
7X-RAY DIFFRACTION7BD1 - 1131 - 123
8X-RAY DIFFRACTION8BD114 - 228124 - 224
9X-RAY DIFFRACTION9CE1 - 1071 - 109
10X-RAY DIFFRACTION10CE108 - 213110 - 215
11X-RAY DIFFRACTION11DF1 - 1131 - 123
12X-RAY DIFFRACTION12DF114 - 228124 - 224
13X-RAY DIFFRACTION13EG1 - 1071 - 109
14X-RAY DIFFRACTION14EG108 - 213110 - 215
15X-RAY DIFFRACTION15FH1 - 1131 - 123
16X-RAY DIFFRACTION16FH114 - 228124 - 224

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