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- PDB-3cd1: Development of a family of redox-sensitive green fluorescent prot... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3cd1 | ||||||
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Title | Development of a family of redox-sensitive green fluorescent protein indicators for use in relatively oxidizing subcellular environments | ||||||
![]() | Green fluorescent protein | ||||||
![]() | LUMINESCENT PROTEIN / GFP / redox | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lohman, J.R. | ||||||
![]() | ![]() Title: Development of a family of redox-sensitive green fluorescent protein indicators for use in relatively oxidizing subcellular environments Authors: Lohman, J.R. / Remington, S.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 107.6 KB | Display | ![]() |
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PDB format | ![]() | 87.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 426.2 KB | Display | ![]() |
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Full document | ![]() | 427.2 KB | Display | |
Data in XML | ![]() | 13.4 KB | Display | |
Data in CIF | ![]() | 19.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28138.557 Da / Num. of mol.: 1 Mutation: C48S,F64L,F99S,S147CR,H148S,M153T,V163A,I167T,Q204C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.95 Å3/Da / Density % sol: 36.97 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 0.1M tris, 24% PEG 1550, 0.04M sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Aug 11, 2007 |
Radiation | Monochromator: Single crystal, cylindrically bent, Si(220) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.312→50 Å / Num. all: 52564 / Num. obs: 52564 / % possible obs: 98.6 % / Observed criterion σ(I): 2 |
Reflection shell | Resolution: 1.312→1.36 Å / % possible all: 92.4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 11.172 Å2
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Refinement step | Cycle: LAST / Resolution: 1.312→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.312→1.346 Å / Total num. of bins used: 20
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