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- PDB-3bbb: Crystal structure of the NM23-H2 transcription factor complex wit... -
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Basic information
Entry | Database: PDB / ID: 3bbb | |||||||||
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Title | Crystal structure of the NM23-H2 transcription factor complex with dinucleotide d(AG) | |||||||||
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![]() | TRANSFERASE / transcription factor / cancer / nm23 gen / hexamer / Activator / Anti-oncogene / ATP-binding / Cell cycle / DNA-binding / Kinase / Magnesium / Metal-binding / Nucleotide metabolism / Nucleotide-binding / Nucleus / Phosphorylation / Transcription regulation | |||||||||
Function / homology | ![]() regulation of epidermis development / nucleoside triphosphate biosynthetic process / Ribavirin ADME / G-quadruplex DNA binding / nucleoside-diphosphate kinase / positive regulation of keratinocyte differentiation / Interconversion of nucleotide di- and triphosphates / UTP biosynthetic process / CTP biosynthetic process / Azathioprine ADME ...regulation of epidermis development / nucleoside triphosphate biosynthetic process / Ribavirin ADME / G-quadruplex DNA binding / nucleoside-diphosphate kinase / positive regulation of keratinocyte differentiation / Interconversion of nucleotide di- and triphosphates / UTP biosynthetic process / CTP biosynthetic process / Azathioprine ADME / nucleoside diphosphate kinase activity / GTP biosynthetic process / histidine kinase / phosphorelay sensor kinase activity / ruffle / positive regulation of epithelial cell proliferation / cell periphery / integrin-mediated signaling pathway / GDP binding / lamellipodium / secretory granule lumen / regulation of apoptotic process / ficolin-1-rich granule lumen / transcription coactivator activity / cell adhesion / Neutrophil degranulation / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / perinuclear region of cytoplasm / positive regulation of transcription by RNA polymerase II / DNA binding / extracellular exosome / extracellular region / ATP binding / identical protein binding / nucleus / metal ion binding / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() synthetic construct (others) | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Weichsel, A. / Montfort, W.R. | |||||||||
![]() | ![]() Title: NM23-H2 may play an indirect role in transcriptional activation of c-myc gene expression but does not cleave the nuclease hypersensitive element III1. Authors: Dexheimer, T.S. / Carey, S.S. / Zuohe, S. / Gokhale, V.M. / Hu, X. / Murata, L.B. / Maes, E.M. / Weichsel, A. / Sun, D. / Meuillet, E.J. / Montfort, W.R. / Hurley, L.H. #1: ![]() Title: X-ray structure of human nucleoside diphosphate kinase B complexed with GDP at 2 A resolution. Authors: Morera, S. / Lacombe, M.L. / Xu, Y. / LeBras, G. / Janin, J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 390.5 KB | Display | ![]() |
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PDB format | ![]() | 320.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 486.7 KB | Display | ![]() |
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Full document | ![]() | 499.8 KB | Display | |
Data in XML | ![]() | 40.8 KB | Display | |
Data in CIF | ![]() | 59.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3bbcC ![]() 3bbfC ![]() 1nueS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17192.859 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P22392, nucleoside-diphosphate kinase, histidine kinase #2: DNA chain | Mass: 597.455 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.92 Å3/Da / Density % sol: 35.79 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: PEG 1500, 50 mM sodium citrate, 20 mM MgCl2, 5 mM DTT, Crystals soaked with 10 mM d(AG), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Dec 13, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 1.3→27 Å / Num. obs: 195789 / % possible obs: 99.3 % / Observed criterion σ(I): 0 / Redundancy: 8 % / Rmerge(I) obs: 0.086 / Net I/σ(I): 16.4 |
Reflection shell | Resolution: 1.3→1.35 Å / Redundancy: 6.8 % / Rmerge(I) obs: 0.42 / Mean I/σ(I) obs: 2.3 / % possible all: 98.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1NUE Resolution: 1.3→27 Å / Cor.coef. Fo:Fc: 0.97 / Cor.coef. Fo:Fc free: 0.956 / SU B: 2.02 / SU ML: 0.039 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.063 / ESU R Free: 0.062 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 14.16 Å2
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Refinement step | Cycle: LAST / Resolution: 1.3→27 Å
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