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- PDB-5iol: Crystal structure of Nucleoside Diphosphate Kinase from Schistoso... -

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Basic information

Entry
Database: PDB / ID: 5iol
TitleCrystal structure of Nucleoside Diphosphate Kinase from Schistosoma mansoni
ComponentsNucleoside diphosphate kinase
KeywordsTRANSFERASE / Nucleoside Diphosphate Kinase / enzyme
Function / homology
Function and homology information


nucleoside-diphosphate kinase / CTP biosynthetic process / UTP biosynthetic process / GTP biosynthetic process / nucleoside diphosphate kinase activity
Similarity search - Function
Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / Alpha-Beta Plaits / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Nucleoside diphosphate kinase
Similarity search - Component
Biological speciesSchistosoma mansoni (invertebrata)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.741 Å
AuthorsTorini, J.R.S. / Romanello, L. / Bird, L.E. / Nettleship, J.E. / Owens, R.J. / Aller, P. / DeMarco, R. / Brandao-Neto, J. / Pereira, H.M.
Funding support Brazil, 2items
OrganizationGrant numberCountry
Sao Paulo Research Foundation (FAPESP)2012/14223-9 Brazil
Brazilian National Council for Scientific and Technological Development (CNPq)474402/2013-4 Brazil
CitationJournal: Mol.Biochem.Parasitol. / Year: 2019
Title: Characterization of a Schistosoma mansoni NDPK expressed in sexual and digestive organs.
Authors: Torini, J.R. / de Freitas Fernandes, A. / Balasco Serrao, V.H. / Romanello, L. / Bird, L.E. / Nettleship, J.E. / Owens, R.J. / Brandao-Neto, J. / Zeraik, A.E. / DeMarco, R. / D'Muniz Pereira, H.
History
DepositionMar 8, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 8, 2017Provider: repository / Type: Initial release
Revision 1.1Apr 17, 2019Group: Author supporting evidence / Data collection / Category: diffrn_source / pdbx_audit_support
Item: _diffrn_source.pdbx_synchrotron_site / _pdbx_audit_support.funding_organization
Revision 1.2May 29, 2019Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.3Jan 1, 2020Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.4Sep 27, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Nucleoside diphosphate kinase
B: Nucleoside diphosphate kinase
C: Nucleoside diphosphate kinase
D: Nucleoside diphosphate kinase
E: Nucleoside diphosphate kinase
F: Nucleoside diphosphate kinase
G: Nucleoside diphosphate kinase
H: Nucleoside diphosphate kinase
I: Nucleoside diphosphate kinase
J: Nucleoside diphosphate kinase
K: Nucleoside diphosphate kinase
L: Nucleoside diphosphate kinase


Theoretical massNumber of molelcules
Total (without water)202,74412
Polymers202,74412
Non-polymers00
Water36,8412045
1
A: Nucleoside diphosphate kinase
B: Nucleoside diphosphate kinase
C: Nucleoside diphosphate kinase
E: Nucleoside diphosphate kinase
F: Nucleoside diphosphate kinase
K: Nucleoside diphosphate kinase


Theoretical massNumber of molelcules
Total (without water)101,3726
Polymers101,3726
Non-polymers00
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area17730 Å2
ΔGint-75 kcal/mol
Surface area32890 Å2
MethodPISA
2
D: Nucleoside diphosphate kinase
G: Nucleoside diphosphate kinase
H: Nucleoside diphosphate kinase
I: Nucleoside diphosphate kinase
J: Nucleoside diphosphate kinase
L: Nucleoside diphosphate kinase


Theoretical massNumber of molelcules
Total (without water)101,3726
Polymers101,3726
Non-polymers00
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area17760 Å2
ΔGint-73 kcal/mol
Surface area33540 Å2
MethodPISA
Unit cell
Length a, b, c (Å)211.760, 82.270, 122.370
Angle α, β, γ (deg.)90.00, 104.89, 90.00
Int Tables number5
Space group name H-MC121

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Components

#1: Protein
Nucleoside diphosphate kinase


Mass: 16895.359 Da / Num. of mol.: 12
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Schistosoma mansoni (invertebrata) / Gene: Smp_092750 / Plasmid: pOPINF / Production host: Escherichia coli (E. coli) / Strain (production host): Lemo21 / References: UniProt: G4VJY9, nucleoside-diphosphate kinase
#2: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 2045 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.54 Å3/Da / Density % sol: 51.58 % / Description: Large plate
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 7.5
Details: 10% PEG 20000, 20%PEG MME 550 100 mM MOPS/HEPES-Na pH 7.5. 20mM of each sodiuml-glutamate, DL-alanine, glycine, DL-lysine HCl, DL-serine

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.92 Å
DetectorType: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Apr 15, 2012
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.92 Å / Relative weight: 1
ReflectionResolution: 1.74→59.13 Å / Num. obs: 204192 / % possible obs: 98.4 % / Redundancy: 3.4 % / Biso Wilson estimate: 21.6 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.07 / Net I/σ(I): 11.2
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsDiffraction-ID% possible all
1.74-1.793.30.7041.9198.3
7.78-59.133.50.03132.5197.2

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
PHENIX1.10.1_2155refinement
xia2data scaling
PHASERphasing
PDB_EXTRACT3.2data extraction
xia2data reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1NDL
Resolution: 1.741→59.13 Å / SU ML: 0.2 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 19.29 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.1909 10267 5.03 %Random selection
Rwork0.163 ---
obs0.1644 204169 98.23 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 1.741→59.13 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms14160 0 0 2045 16205
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00514527
X-RAY DIFFRACTIONf_angle_d0.71619607
X-RAY DIFFRACTIONf_dihedral_angle_d14.7488827
X-RAY DIFFRACTIONf_chiral_restr0.0492082
X-RAY DIFFRACTIONf_plane_restr0.0042561
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.7405-1.76030.29463080.28256426X-RAY DIFFRACTION98
1.7603-1.7810.29523280.27416489X-RAY DIFFRACTION98
1.781-1.80270.30573260.25816405X-RAY DIFFRACTION97
1.8027-1.82550.27643450.24526269X-RAY DIFFRACTION96
1.8255-1.84960.28393240.23966411X-RAY DIFFRACTION98
1.8496-1.87490.25393350.22756562X-RAY DIFFRACTION100
1.8749-1.90170.26263120.21816504X-RAY DIFFRACTION100
1.9017-1.93010.23623520.20576524X-RAY DIFFRACTION99
1.9301-1.96020.2173820.18926543X-RAY DIFFRACTION99
1.9602-1.99240.21533670.18756450X-RAY DIFFRACTION99
1.9924-2.02670.223450.18456521X-RAY DIFFRACTION99
2.0267-2.06360.21633580.17996468X-RAY DIFFRACTION99
2.0636-2.10330.21423400.18246497X-RAY DIFFRACTION99
2.1033-2.14620.21023780.17246438X-RAY DIFFRACTION99
2.1462-2.19290.20173740.16856425X-RAY DIFFRACTION99
2.1929-2.24390.20113380.15726479X-RAY DIFFRACTION99
2.2439-2.30.22673250.1636464X-RAY DIFFRACTION98
2.3-2.36220.19163610.16026399X-RAY DIFFRACTION98
2.3622-2.43170.21113290.15756420X-RAY DIFFRACTION98
2.4317-2.51020.18063380.16146443X-RAY DIFFRACTION97
2.5102-2.59990.18043440.15886225X-RAY DIFFRACTION95
2.5999-2.7040.19423330.15366492X-RAY DIFFRACTION98
2.704-2.82710.19873470.16476532X-RAY DIFFRACTION99
2.8271-2.97610.20763610.16766509X-RAY DIFFRACTION99
2.9761-3.16260.18243320.15646544X-RAY DIFFRACTION99
3.1626-3.40670.17793280.15446499X-RAY DIFFRACTION98
3.4067-3.74950.16123460.14196470X-RAY DIFFRACTION98
3.7495-4.2920.14633450.13166467X-RAY DIFFRACTION97
4.292-5.40680.14643320.12836370X-RAY DIFFRACTION95
5.4068-59.16460.16683340.15186657X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
13.1928-2.99531.13154.7404-1.65972.2399-0.0766-0.01880.21490.2456-0.0144-0.144-0.0988-0.07220.08510.1261-0.0349-0.00170.2092-0.05230.1255111.1216-10.218851.6295
22.5268-2.6481.36077.5296-5.17543.7653-0.06960.0992-0.0101-0.2299-0.0466-0.01120.0532-0.0350.01040.16670.0005-0.00090.2744-0.06570.1592113.0834-12.607442.2974
32.9330.26710.83351.95050.36913.6850.0487-0.12960.12160.3713-0.05140.06630.10320.18010.02230.1873-0.00360.02890.1376-0.08710.1756112.6268-5.0256.0798
49.11080.3294-4.10512.53-1.6596.833-0.2766-0.0155-0.2680.63280.10820.59890.3259-0.72950.19020.3839-0.07770.13110.3106-0.0940.2947106.7-12.515266.7075
51.4257-0.72670.6725.95550.13721.14840.0396-0.09090.07810.03250.03140.47650.0903-0.1581-0.10410.13680.00720.02730.257-0.0510.1177103.5285-10.231847.0044
62.0945-1.10350.20786.7475-2.25451.4354-0.0304-0.0602-0.0692-0.10530.07890.1830.1424-0.27020.030.1892-0.04660.02320.2865-0.04150.1709105.6079-18.737951.422
74.2655-0.10625.95773.0248-0.28758.3625-0.224-0.92490.59040.2508-0.0560.2479-0.2385-0.6260.26560.21640.01190.01430.2869-0.10850.2358103.98780.064555.7859
80.66220.45041.84730.4350.28166.4019-0.257-0.13780.2834-0.0359-0.19060.0435-0.6028-0.18140.50270.23130.0440.01230.2509-0.07180.3185111.84173.227540.0284
92.41320.4753-0.61434.615-0.25121.22870.06480.0942-0.0559-0.1255-0.15520.02120.0979-0.05950.08620.12940.04160.00290.2028-0.04270.0943105.9505-17.598922.6738
102.40770.0732-1.3751.9215-0.77932.18720.18650.46410.0761-0.2032-0.19050.0449-0.1148-0.0330.01790.16140.054-0.04920.2717-0.05450.112102.0736-14.449113.8509
112.154-1.5888-0.15751.9652-0.12510.23290.10670.23140.0142-0.104-0.06040.12230.06390.0384-0.05730.17140.0492-0.03150.2416-0.04210.149296.6371-10.586818.5516
121.66830.93590.76738.15965.35573.5316-0.0762-0.2440.179-0.145-0.55411.1585-0.4057-0.74150.61030.22790.0609-0.02290.3434-0.05190.295996.0838-18.578529.4457
132.63291.340.23923.63091.44812.67620.0893-0.074-0.00430.1666-0.08530.3081-0.0229-0.07610.01790.150.05380.0030.2321-0.05420.1827100.2637-12.695529.7514
141.6907-2.79730.58574.7337-1.37210.6260.30980.2987-0.2337-0.3077-0.40620.31790.0758-0.06360.09460.22160.0417-0.07280.3397-0.13730.2447101.0298-27.473113.743
152.0206-1.14130.90183.461-2.00632.82230.0018-0.27110.16210.0201-0.0084-0.0724-0.04030.08190.0140.1146-0.01280.02170.2233-0.06160.1313125.6561-9.545642.6844
161.6451-0.3854-0.6911.4566-0.14750.87150.1225-0.27440.44950.08370.0071-0.1079-0.17150.0421-0.08550.1569-0.0236-0.02230.2449-0.11560.262131.18271.167743.5831
173.5949-2.26274.155.55180.48817.53020.1482-0.2515-0.12630.02210.0623-0.39890.20190.2097-0.13890.1302-0.02480.01280.26520.00240.1957136.2225-16.260244.8038
182.39460.55961.10731.39530.22842.69090.0235-0.07590.1393-0.0182-0.077-0.22130.03020.29410.04690.1396-0.01730.02240.2424-0.02410.2016133.516-7.065638.1945
190.95340.48590.11827.75440.51220.9349-0.1553-0.70290.0920.63530.3441-0.1346-0.03570.045-0.11060.214-0.0205-0.04680.4954-0.08750.1898127.559-15.62958.0447
202.4982-0.71651.28380.6968-0.68782.7476-0.0471-0.09870.0715-0.03120.0434-0.0063-0.0648-0.00750.00750.1604-0.00910.00550.0906-0.00390.1177177.48-5.357225.6101
214.4418-1.26582.19911.47051.19944.15950.35440.72710.9084-1.0187-0.19190.0576-0.55950.7479-0.03640.5407-0.085-0.04850.15310.07450.3324181.308811.291517.2708
226.2412.0862-4.73571.8924-2.89535.0535-0.22-0.00780.23680.0056-0.0536-0.04430.10820.3840.23970.2879-0.0497-0.00420.21210.02260.1853184.77316.622210.7892
232.1482-0.51231.40392.2827-0.63522.46340.00530.10830.0082-0.1545-0.0523-0.2218-0.01890.2361-0.00410.1502-0.0320.0140.08790.01020.1689185.6352-7.993624.1445
242.43760.88312.09283.37870.89325.8865-0.03550.1278-0.0114-0.215-0.0384-0.27360.17720.29980.05160.1649-0.00760.04180.11640.01520.1465182.7318-9.367415.1278
259.8547-5.3143-0.23367.932-0.42527.30110.1832-0.57930.38060.0313-0.0865-0.6696-0.28280.77150.01170.2452-0.1226-0.00660.21920.01620.217187.90925.069327.1903
260.2964-1.01340.30866.0264-4.21393.8469-0.0552-0.10390.11730.5942-0.0378-0.2262-0.45830.05910.08370.2333-0.0683-0.03950.2716-0.03340.1821180.1024-4.719240.2349
272.6277-1.417-1.75064.30492.98145.3673-0.0141-0.2169-0.2720.01440.0038-0.09650.1133-0.03690.03610.1193-0.0192-0.00910.2080.05210.1989127.09-38.468842.3901
283.34960.986-1.63340.54690.21427.0759-0.034-0.0182-0.0227-0.0717-0.0578-0.021-0.1807-0.0630.10370.12260.0138-0.01240.17090.01130.17122.0411-36.531134.1293
292.4006-0.22930.73330.8362-0.08312.80530.0217-0.4338-0.34980.0665-0.0174-0.08670.09710.1341-0.02160.17870.0013-0.00480.23660.10380.2909133.3192-44.061447.5762
302.17913.72612.83159.50236.985.2020.0495-0.1372-0.23290.28260.3887-0.56750.01930.496-0.49590.21130.0155-0.00320.33180.00710.2537135.5339-32.2835.9476
311.5192-0.7287-0.55263.54231.91912.7188-0.0326-0.1646-0.0017-0.05510.0073-0.0889-0.12610.21250.02850.1544-0.0227-0.02910.26040.03660.2251132.9751-30.336142.3113
326.1360.8565-6.50394.2620.27399.8136-0.2199-0.01-0.71430.00050.06280.09940.35670.28180.14740.1690.0349-0.02820.2390.04110.318134.0759-49.467638.1836
333.5076-5.07143.44557.5456-5.08933.45710.21130.2438-0.5389-0.3520.0496-0.02850.65070.0639-0.44380.2249-0.0276-0.01550.2925-0.0010.4051120.5196-51.084733.0899
345.59214.9332-4.44166.7319-3.25453.74510.0969-0.068-0.90650.0816-0.4248-0.36430.75920.57410.42670.2550.08780.00270.2305-0.0080.2738120.571-39.420517.8688
351.89440.282-0.04883.97731.13921.20840.12520.13080.0702-0.1736-0.1375-0.0536-0.1514-0.16380.02030.14150.05920.00790.1852-0.00540.0975119.3603-18.108817.5947
363.74223.9758-2.9726.3602-0.36577.5272-0.11460.71250.0188-0.42610.01690.0828-0.0627-0.63920.15330.25240.0943-0.00220.2597-0.04040.1377121.0827-26.01790.7367
371.9254-0.8364-1.01172.89221.95432.20480.03890.0684-0.1038-0.0892-0.0811-0.0483-0.1242-0.17980.0260.18680.0576-0.01130.15430.00820.0964125.1681-27.03846.6585
381.1507-0.1242-0.2118.6744-2.31072.01440.0474-0.04310.04510.0035-0.0749-0.66670.00020.13180.05520.17150.02650.01610.2335-0.02070.1952130.2956-21.339322.2553
391.44480.2305-1.2791.46160.77423.29790.16380.04840.0965-0.3154-0.092-0.1938-0.3602-0.0287-0.05640.19690.06650.04010.1606-0.00060.1404126.8447-21.476910.9018
400.2004-0.9378-0.45863.65992.04012.25290.12060.30090.1633-0.6587-0.2519-0.1335-0.45750.16010.05550.26170.09520.06990.31520.02830.2561118.2313-3.514217.2721
411.41990.379-1.53696.36035.45997.95360.0806-0.32770.01040.4669-0.1-0.01920.3202-0.2551-0.07160.19510.0007-0.01210.27620.05240.1647162.3048-33.423944.1018
420.47870.0068-0.17991.51861.77972.3328-0.0269-0.0283-0.0617-0.22240.05060.0155-0.17820.00580.00150.15790.002-0.00760.14490.0350.1627166.3697-32.549831.3889
431.5867-1.8678-0.55193.89080.14070.7506-0.1-0.2921-0.13470.0910.09910.0460.1210.14460.00420.1529-0.0094-0.01010.19190.05690.124169.5865-33.915142.2184
443.93510.08921.46742.3659-0.70281.05550.226-0.3403-0.55960.1616-0.2751-0.02160.1467-0.1225-0.19720.2172-0.02850.0110.21470.0860.2576155.7534-49.88940.6014
450.94570.0241-0.10461.26140.69240.37360.0005-0.1308-0.27190.0237-0.0070.02850.057-0.00530.02510.1945-0.0009-0.00990.16850.08270.2128162.0239-44.381736.5153
464.28090.13560.44692.17222.35257.08040.0507-0.52590.14050.0472-0.03150.3684-0.2506-0.35940.2370.1709-0.00260.00270.20090.01860.1628156.6297-26.230740.953
475.99090.44172.15455.90661.49095.9695-0.2745-0.00460.2238-0.1319-0.0110.5643-0.1886-0.38370.0620.20370.011-0.03190.19740.03210.218154.5202-31.609627.0538
480.9061-0.0644-0.14061.20330.22271.89160.0265-0.2039-0.11320.1133-0.01960.13270.1449-0.1849-0.13560.151-0.0341-0.00320.20670.05920.1788157.5233-38.945339.439
490.0432-0.32140.29472.782-2.16531.5404-0.0581-0.1849-0.04240.27490.1032-0.0555-0.0688-0.02640.06090.1912-0.01950.00510.27820.02970.1337170.2722-25.719249.1557
503.5942-0.77211.82312.1612-0.91375.1288-0.06320.0050.1751-0.1167-0.00270.0439-0.0617-0.07150.07020.1203-0.0250.00220.0552-0.00530.1048163.7564-6.796529.1197
511.76090.52730.34030.51410.43932.41450.0223-0.40390.31130.0428-0.10660.1527-0.1606-0.17010.06380.18350.0218-0.01520.1727-0.07630.2038160.52661.102937.5729
528.2196-1.48522.37085.2604-5.04215.06670.08840.04240.0718-0.32860.14550.49610.2061-0.5802-0.17810.18610.0133-0.03260.1522-0.02620.1713152.5549-7.50123.6728
532.3176-1.44120.93344.0489-1.35792.3343-0.039-0.26940.0395-0.02770.01990.2194-0.0014-0.23410.00650.15580.00140.01540.2029-0.03480.1525156.2609-7.634434.6395
540.531-0.23070.32510.0730.29973.0084-0.1697-0.04330.2102-0.2141-0.07570.2959-0.43760.0310.25460.24510.0156-0.03440.120.0110.2217161.84961.396114.8234
552.41830.8494-1.30613.9263-1.62583.37110.02870.2121-0.0257-0.17650.02070.1098-0.04330.0625-0.0690.13520.0063-0.02170.1076-0.01490.1141174.7549-30.65135.1275
560.74321.6386-0.40195.4218-0.88760.25130.095-0.02970.09030.0452-0.08050.1611-0.13590.0196-0.03720.1890.0021-0.01280.1646-0.00890.1252172.9838-22.41911.1183
572.66363.8511.41127.95792.54391.8691-0.11770.1770.0283-0.35890.10150.104-0.06580.11760.11920.17770.00970.00560.1401-0.00690.1008169.7199-24.85530.7933
586.3452-0.55690.98343.44420.08430.44040.17280.709-0.3353-0.2785-0.11-0.3406-0.05170.266-0.05130.2793-0.01580.0490.2199-0.08440.2407181.7261-41.7086-5.013
591.55410.2789-0.30742.4643-0.18511.5556-0.14150.0374-0.28880.08520.157-0.09660.03780.05580.18470.161-0.0005-0.01230.1278-0.04140.1341175.9782-37.54721.3731
605.6151-0.85631.84598.4397-3.94537.61180.03720.30260.24820.0235-0.0328-0.3599-0.46970.5990.13060.184-0.00440.03550.16760.00630.1718183.177-19.53464.9391
611.7491-0.35161.19642.911-1.81321.8492-0.02550.0822-0.0337-0.07040.0127-0.28870.00340.188-0.00330.13180.01920.0170.1346-0.01590.1419183.5298-32.316312.0825
627.2055.09150.52583.61250.41163.1474-0.35390.4299-0.2734-0.66220.1951-0.3440.10960.21720.15270.34240.0190.05640.1839-0.02090.1854180.1982-28.2934-7.1878
630.54580.0080.25357.42722.65661.2362-0.07160.15050.0432-0.58710.0350.0196-0.23070.12510.03760.2907-0.00720.0310.20670.04160.1721170.3733-14.0775-1.8867
642.84042.044-0.04455.9758-1.05322.3178-0.01460.0757-0.2042-0.21510.00680.02250.1092-0.08030.02430.12770.0225-0.01820.0801-0.01830.11157.4136-24.19966.9902
652.653.3376-2.31557.5052-4.2812.6229-0.0088-0.1265-0.07770.2419-0.06-0.2211-0.06810.01990.10120.19340.0083-0.02370.1355-0.01580.118162.384-27.697615.3092
660.98310.2283-1.15812.0209-1.47283.7611-0.01580.0403-0.1559-0.1943-0.0671-0.05270.13060.17170.10590.17770.0031-0.04060.1117-0.01950.1575161.0724-31.85925.1679
676.9695-2.0609-0.9151.5827-0.49340.91990.23930.3291-0.1266-0.2158-0.12120.22730.0003-0.021-0.08790.24790.0127-0.07080.1437-0.0270.1512145.4715-20.5597-3.3249
681.74410.3794-0.09920.4972-0.69491.77880.0380.11330.2015-0.051-0.0768-0.0209-0.00180.04070.03120.16430.0058-0.03870.0949-0.0340.1167153.6767-20.66991.0725
695.26730.9021-1.67776.13822.47625.77410.0283-0.0037-0.29140.3883-0.08140.01160.2396-0.47740.03760.1352-0.0257-0.03080.13010.02370.1345151.0527-32.946716.6643
705.1080.88520.15968.07591.89918.8416-0.1641-0.3670.17230.23320.02690.561-0.096-0.51220.13560.23850.0332-0.01260.15970.00620.1907152.4484-17.3418.4512
712.24311.9725-0.4866.3089-0.12371.22250.02340.0369-0.0437-0.16860.01460.1794-0.0303-0.23920.01150.1740.0203-0.03350.1288-0.00840.1585151.2822-18.165210.0117
728.66274.4743-6.94032.3963-4.13568.9947-0.0926-0.0293-0.3773-0.08950.02020.01650.3996-0.07410.07280.20040.0143-0.07060.096-0.01650.2411148.0681-32.64751.4442
734.3187-4.25025.12665.2356-5.49537.0220.46290.1314-0.2083-0.8091-0.35240.26010.8857-0.0156-0.17670.2747-0.0011-0.0430.1483-0.04940.2653160.9275-40.01891.9314
745.3887-2.4534-2.1762.3149-0.04395.9067-0.12680.762-0.5104-0.1716-0.12170.0525-0.0503-0.94730.09320.1812-0.0269-0.02070.2-0.01940.2801161.1955-45.374920.6809
751.75050.47270.35840.74490.17850.52560.0555-0.1509-0.2160.0614-0.027-0.04910.0752-0.0811-0.0330.1621-0.0197-0.00010.19440.02720.1947106.5639-43.357840.6477
762.9231-1.02822.46082.3772-1.04495.1267-0.0684-0.002-0.0129-0.0725-0.02270.2282-0.2169-0.70870.02550.1511-0.0145-0.00860.2786-0.01590.2284100.6191-31.390739.5011
771.4835-0.1527-0.24821.7372-1.25423.09330.0592-0.2145-0.22430.10410.01230.14430.0547-0.3134-0.07480.1592-0.0633-0.02650.27250.01440.2342101.6443-43.193842.3714
781.73572.91812.91685.14764.78364.52070.2183-0.2325-0.26710.7224-0.0441-0.3110.3884-0.0852-0.09940.2281-0.02930.03480.38440.06870.219115.8836-37.822156.5388
791.3204-0.0905-0.4841.30961.52643.1306-0.0203-0.0649-0.09520.0115-0.0107-0.0120.06540.04340.04310.11-0.007-0.0150.09760.0460.116179.6068-33.258432.3959
805.642-0.01741.39333.98671.00193.0137-0.0058-0.29420.04440.29910.1354-0.81960.00170.5014-0.0830.21480.0161-0.07960.31780.01820.2694191.5474-31.236747.5634
810.99660.0187-0.03860.54660.32640.6316-0.0205-0.02350.0214-0.02530.0125-0.0575-0.01270.10710.00790.16990.0119-0.01220.15190.03040.1815186.3777-32.143230.7006
821.9268-0.3832-0.05825.95021.8962.00090.0281-0.0970.0933-0.18920.026-0.146-0.11010.2578-0.02590.1503-0.037-0.01030.1830.0210.1603186.8666-23.661732.6231
834.94551.2902-6.19632.3938-0.35618.719-0.0487-0.4555-0.51790.1717-0.0283-0.20890.26790.96710.08790.1910.0785-0.05230.25740.050.2294190.9516-41.83437.9901
848.61980.4965.51260.76110.84677.37010.2559-0.357-0.53960.3433-0.0590.3580.7521-0.29-0.20150.27330.02590.01990.14020.04240.329177.6342-47.906834.7136
857.48762.9776-4.99243.5712-1.96869.1692-0.1414-0.5537-0.9640.14-0.0404-0.21390.42380.33460.21450.23790.01940.00520.08160.01420.3006176.7752-44.900915.6146
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 17 )
2X-RAY DIFFRACTION2chain 'A' and (resid 18 through 27 )
3X-RAY DIFFRACTION3chain 'A' and (resid 28 through 48 )
4X-RAY DIFFRACTION4chain 'A' and (resid 49 through 70 )
5X-RAY DIFFRACTION5chain 'A' and (resid 71 through 95 )
6X-RAY DIFFRACTION6chain 'A' and (resid 96 through 119 )
7X-RAY DIFFRACTION7chain 'A' and (resid 120 through 130 )
8X-RAY DIFFRACTION8chain 'A' and (resid 131 through 148 )
9X-RAY DIFFRACTION9chain 'B' and (resid 1 through 27 )
10X-RAY DIFFRACTION10chain 'B' and (resid 28 through 48 )
11X-RAY DIFFRACTION11chain 'B' and (resid 49 through 79 )
12X-RAY DIFFRACTION12chain 'B' and (resid 80 through 95 )
13X-RAY DIFFRACTION13chain 'B' and (resid 96 through 119 )
14X-RAY DIFFRACTION14chain 'B' and (resid 120 through 149 )
15X-RAY DIFFRACTION15chain 'C' and (resid 1 through 27 )
16X-RAY DIFFRACTION16chain 'C' and (resid 28 through 76 )
17X-RAY DIFFRACTION17chain 'C' and (resid 77 through 88 )
18X-RAY DIFFRACTION18chain 'C' and (resid 89 through 130 )
19X-RAY DIFFRACTION19chain 'C' and (resid 131 through 148 )
20X-RAY DIFFRACTION20chain 'D' and (resid 0 through 38 )
21X-RAY DIFFRACTION21chain 'D' and (resid 39 through 48 )
22X-RAY DIFFRACTION22chain 'D' and (resid 49 through 69 )
23X-RAY DIFFRACTION23chain 'D' and (resid 70 through 95 )
24X-RAY DIFFRACTION24chain 'D' and (resid 96 through 119 )
25X-RAY DIFFRACTION25chain 'D' and (resid 120 through 130 )
26X-RAY DIFFRACTION26chain 'D' and (resid 131 through 149 )
27X-RAY DIFFRACTION27chain 'E' and (resid 1 through 17 )
28X-RAY DIFFRACTION28chain 'E' and (resid 18 through 35 )
29X-RAY DIFFRACTION29chain 'E' and (resid 36 through 79 )
30X-RAY DIFFRACTION30chain 'E' and (resid 80 through 95 )
31X-RAY DIFFRACTION31chain 'E' and (resid 96 through 119 )
32X-RAY DIFFRACTION32chain 'E' and (resid 120 through 130 )
33X-RAY DIFFRACTION33chain 'E' and (resid 131 through 141 )
34X-RAY DIFFRACTION34chain 'E' and (resid 142 through 149 )
35X-RAY DIFFRACTION35chain 'F' and (resid 0 through 38 )
36X-RAY DIFFRACTION36chain 'F' and (resid 39 through 48 )
37X-RAY DIFFRACTION37chain 'F' and (resid 49 through 76 )
38X-RAY DIFFRACTION38chain 'F' and (resid 77 through 105 )
39X-RAY DIFFRACTION39chain 'F' and (resid 106 through 130 )
40X-RAY DIFFRACTION40chain 'F' and (resid 131 through 149 )
41X-RAY DIFFRACTION41chain 'G' and (resid 1 through 8 )
42X-RAY DIFFRACTION42chain 'G' and (resid 9 through 28 )
43X-RAY DIFFRACTION43chain 'G' and (resid 29 through 38 )
44X-RAY DIFFRACTION44chain 'G' and (resid 39 through 57 )
45X-RAY DIFFRACTION45chain 'G' and (resid 58 through 76 )
46X-RAY DIFFRACTION46chain 'G' and (resid 77 through 88 )
47X-RAY DIFFRACTION47chain 'G' and (resid 89 through 105 )
48X-RAY DIFFRACTION48chain 'G' and (resid 106 through 130 )
49X-RAY DIFFRACTION49chain 'G' and (resid 131 through 148 )
50X-RAY DIFFRACTION50chain 'H' and (resid 0 through 27 )
51X-RAY DIFFRACTION51chain 'H' and (resid 28 through 76 )
52X-RAY DIFFRACTION52chain 'H' and (resid 77 through 88 )
53X-RAY DIFFRACTION53chain 'H' and (resid 89 through 130 )
54X-RAY DIFFRACTION54chain 'H' and (resid 131 through 149 )
55X-RAY DIFFRACTION55chain 'I' and (resid 1 through 17 )
56X-RAY DIFFRACTION56chain 'I' and (resid 18 through 28 )
57X-RAY DIFFRACTION57chain 'I' and (resid 29 through 38 )
58X-RAY DIFFRACTION58chain 'I' and (resid 39 through 57 )
59X-RAY DIFFRACTION59chain 'I' and (resid 58 through 76 )
60X-RAY DIFFRACTION60chain 'I' and (resid 77 through 88 )
61X-RAY DIFFRACTION61chain 'I' and (resid 89 through 119 )
62X-RAY DIFFRACTION62chain 'I' and (resid 120 through 130 )
63X-RAY DIFFRACTION63chain 'I' and (resid 131 through 149 )
64X-RAY DIFFRACTION64chain 'J' and (resid 1 through 17 )
65X-RAY DIFFRACTION65chain 'J' and (resid 18 through 28 )
66X-RAY DIFFRACTION66chain 'J' and (resid 29 through 38 )
67X-RAY DIFFRACTION67chain 'J' and (resid 39 through 57 )
68X-RAY DIFFRACTION68chain 'J' and (resid 58 through 76 )
69X-RAY DIFFRACTION69chain 'J' and (resid 77 through 88 )
70X-RAY DIFFRACTION70chain 'J' and (resid 89 through 105 )
71X-RAY DIFFRACTION71chain 'J' and (resid 106 through 119 )
72X-RAY DIFFRACTION72chain 'J' and (resid 120 through 130 )
73X-RAY DIFFRACTION73chain 'J' and (resid 131 through 141 )
74X-RAY DIFFRACTION74chain 'J' and (resid 142 through 149 )
75X-RAY DIFFRACTION75chain 'K' and (resid 1 through 79 )
76X-RAY DIFFRACTION76chain 'K' and (resid 80 through 105 )
77X-RAY DIFFRACTION77chain 'K' and (resid 106 through 130 )
78X-RAY DIFFRACTION78chain 'K' and (resid 131 through 148 )
79X-RAY DIFFRACTION79chain 'L' and (resid 0 through 35 )
80X-RAY DIFFRACTION80chain 'L' and (resid 36 through 66 )
81X-RAY DIFFRACTION81chain 'L' and (resid 67 through 95 )
82X-RAY DIFFRACTION82chain 'L' and (resid 96 through 119 )
83X-RAY DIFFRACTION83chain 'L' and (resid 120 through 130 )
84X-RAY DIFFRACTION84chain 'L' and (resid 131 through 141 )
85X-RAY DIFFRACTION85chain 'L' and (resid 142 through 149 )

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