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Open data
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Basic information
Entry | Database: PDB / ID: 2zc7 | ||||||
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Title | Crystal Structure of Class C beta-Lactamase ACT-1 | ||||||
![]() | Beta-lactamase ACT-1 | ||||||
![]() | HYDROLASE / two-domain structure | ||||||
Function / homology | ![]() antibiotic catabolic process / beta-lactamase activity / beta-lactamase / outer membrane-bounded periplasmic space / response to antibiotic Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Shimizu-Ibuka, A. / Sakai, H. / Galleni, M. | ||||||
![]() | ![]() Title: Structure of the plasmid-mediated class C beta-lactamase ACT-1 Authors: Shimizu-Ibuka, A. / Bauvois, C. / Sakai, H. / Galleni, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 282.6 KB | Display | ![]() |
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PDB format | ![]() | 229.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 461.8 KB | Display | ![]() |
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Full document | ![]() | 501.8 KB | Display | |
Data in XML | ![]() | 57.4 KB | Display | |
Data in CIF | ![]() | 80.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1xx2S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 39321.953 Da / Num. of mol.: 4 / Fragment: residues in database 23-381 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.85 Å3/Da / Density % sol: 56.81 % |
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Crystal grow | Temperature: 285 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 30% PEG4000, 0.1M Tris-HCl, 0.2M sodium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: May 28, 2003 |
Radiation | Monochromator: Numerical link type Si(111) double crystal monochromator Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. all: 86460 / Num. obs: 86460 / % possible obs: 89.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 3 / Redundancy: 2 % / Rmerge(I) obs: 0.054 / Net I/σ(I): 14.7 |
Reflection shell | Resolution: 2.2→2.28 Å / Rmerge(I) obs: 0.328 / Mean I/σ(I) obs: 30.2 / Num. unique all: 8455 / % possible all: 84.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1XX2 Resolution: 2.4→20 Å / Isotropic thermal model: anisotropic / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refine analyze | Luzzati coordinate error obs: 0.51 Å / Luzzati d res low obs: 20 Å / Luzzati sigma a obs: -0.89 Å | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.51 Å |