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Yorodumi- PDB-1fco: CRYSTAL STRUCTURE OF THE E. COLI AMPC BETA-LACTAMASE COVALENTLY A... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fco | ||||||
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Title | CRYSTAL STRUCTURE OF THE E. COLI AMPC BETA-LACTAMASE COVALENTLY ACYLATED WITH THE INHIBITORY BETA-LACTAM, MOXALACTAM | ||||||
Components | BETA-LACTAMASE | ||||||
Keywords | HYDROLASE / beta-lactamase beta-lactam complex / enzyme inhibitor complex | ||||||
Function / homology | Function and homology information antibiotic catabolic process / beta-lactamase activity / beta-lactamase / outer membrane-bounded periplasmic space / response to antibiotic Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.2 Å | ||||||
Authors | Patera, A. / Blaszczak, L.C. / Shoichet, B.K. | ||||||
Citation | Journal: J.Am.Chem.Soc. / Year: 2000 Title: Crystal Structures of Substrate and Inhibitor Complexes with AmpC -Lactamase: Possible Implications for Substrate-Assisted Catalysis Authors: Patera, A. / Blaszczak, L.C. / Shoichet, B.K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fco.cif.gz | 144.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fco.ent.gz | 120.8 KB | Display | PDB format |
PDBx/mmJSON format | 1fco.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fc/1fco ftp://data.pdbj.org/pub/pdb/validation_reports/fc/1fco | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 39587.922 Da / Num. of mol.: 2 / Mutation: Q120L/Y150E Source method: isolated from a genetically manipulated source Details: INHIBITOR MOXALACTAM, RESIDUE MOX, BINDS TO SER 61 BY BREAKING ITS C1-N1 LACTAM BOND Source: (gene. exp.) Escherichia coli (E. coli) / Plasmid: POGO295 / Production host: Escherichia coli (E. coli) / References: UniProt: P00811, beta-lactamase #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.53 Å3/Da / Density % sol: 51.34 % | |||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.7 Details: 1.7 M potassium phosphate, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |||||||||||||||
Crystal grow | *PLUS Temperature: 23 ℃ | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 129 K |
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Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.2→20 Å / Num. all: 39390 / Num. obs: 39390 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.114 / Net I/σ(I): 12.8 |
Reflection shell | Resolution: 2.2→2.25 Å / Rmerge(I) obs: 0.316 |
Reflection | *PLUS % possible obs: 95 % |
-Processing
Software |
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Refinement | Resolution: 2.2→8 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.2→8 Å
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Software | *PLUS Name: CNS / Classification: refinement | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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