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- PDB-2z7s: Crystal Structure of the N-terminal Kinase Domain of Human RSK1 b... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2z7s | ||||||
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Title | Crystal Structure of the N-terminal Kinase Domain of Human RSK1 bound to Purvalnol A | ||||||
![]() | Ribosomal protein S6 kinase alpha-1 | ||||||
![]() | TRANSFERASE / protein kinase / cancer / kinase inhibitor | ||||||
Function / homology | ![]() regulation of translation in response to stress / CREB1 phosphorylation through NMDA receptor-mediated activation of RAS signaling / ribosomal protein S6 kinase activity / CREB phosphorylation / hepatocyte proliferation / positive regulation of hepatic stellate cell activation / Gastrin-CREB signalling pathway via PKC and MAPK / RSK activation / negative regulation of TOR signaling / ERK/MAPK targets ...regulation of translation in response to stress / CREB1 phosphorylation through NMDA receptor-mediated activation of RAS signaling / ribosomal protein S6 kinase activity / CREB phosphorylation / hepatocyte proliferation / positive regulation of hepatic stellate cell activation / Gastrin-CREB signalling pathway via PKC and MAPK / RSK activation / negative regulation of TOR signaling / ERK/MAPK targets / Recycling pathway of L1 / cysteine-type endopeptidase inhibitor activity involved in apoptotic process / Transcriptional and post-translational regulation of MITF-M expression and activity / protein serine/threonine/tyrosine kinase activity / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / positive regulation of cell differentiation / Senescence-Associated Secretory Phenotype (SASP) / positive regulation of cell growth / chemical synaptic transmission / non-specific serine/threonine protein kinase / intracellular signal transduction / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / synapse / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / magnesium ion binding / signal transduction / positive regulation of transcription by RNA polymerase II / nucleoplasm / ATP binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ikuta, M. / Munshi, S.K. | ||||||
![]() | ![]() Title: Crystal structures of the N-terminal kinase domain of human RSK1 bound to three different ligands: Implications for the design of RSK1 specific inhibitors. Authors: Ikuta, M. / Kornienko, M. / Byrne, N. / Reid, J.C. / Mizuarai, S. / Kotani, H. / Munshi, S.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 68.5 KB | Display | ![]() |
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PDB format | ![]() | 49 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2z7qC ![]() 2z7rC ![]() 1vzoS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 36777.391 Da / Num. of mol.: 1 / Fragment: Residues 33-353 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q15418, non-specific serine/threonine protein kinase |
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#2: Chemical | ChemComp-P01 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 44.58 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 12-16% PEG MME 2000, 150mM DL-malic acid , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Oct 12, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.1→50 Å / Num. obs: 20282 / % possible obs: 96.2 % / Redundancy: 6.4 % / Biso Wilson estimate: 39 Å2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1VZO Resolution: 2.1→20 Å
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Refinement step | Cycle: LAST / Resolution: 2.1→20 Å
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