Mass: 18.015 Da / Num. of mol.: 63 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.82 Å3/Da / Density % sol: 56.03 % Description: THE METHIONINE RESIDUES WERE ASIGNED TO THE SEQUENCE USING THE ANOMALOUS SIGNAL FROM ANOTHER CRYSTAL CONTAINING SEMET AT LOW RESOLUTION.
Crystal grow
pH: 8 Details: LITHIUM SULFATE 180MM, PEG3350 24%, HEPES NA PH8 100MM, TRIS HCL PH7.5 40MM
Resolution: 2.55→30 Å / Cor.coef. Fo:Fc: 0.944 / Cor.coef. Fo:Fc free: 0.923 / SU B: 18.503 / SU ML: 0.179 / Cross valid method: THROUGHOUT / ESU R: 0.104 / ESU R Free: 0.051 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.20162
1544
5 %
RANDOM
Rwork
0.16925
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obs
0.17083
29091
99.83 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK