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- PDB-2xb1: Crystal structure of the human Pygo2 PHD finger in complex with t... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2xb1 | ||||||
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Title | Crystal structure of the human Pygo2 PHD finger in complex with the B9L HD1 domain | ||||||
![]() | PYGOPUS HOMOLOG 2, B-CELL CLL/LYMPHOMA 9-LIKE PROTEIN | ||||||
![]() | TRANSCRIPTION / FUSION PROTEIN / SIGNAL TRANSDUCTION / METAL BINDING / WNT PROTEINS | ||||||
Function / homology | ![]() histone acetyltransferase regulator activity / spermatid nucleus differentiation / regulation of mammary gland epithelial cell proliferation / beta-catenin-TCF complex / mammary gland development / regulation of cell morphogenesis / myoblast differentiation / lens development in camera-type eye / roof of mouth development / skeletal muscle cell differentiation ...histone acetyltransferase regulator activity / spermatid nucleus differentiation / regulation of mammary gland epithelial cell proliferation / beta-catenin-TCF complex / mammary gland development / regulation of cell morphogenesis / myoblast differentiation / lens development in camera-type eye / roof of mouth development / skeletal muscle cell differentiation / somatic stem cell population maintenance / developmental growth / canonical Wnt signaling pathway / positive regulation of epithelial to mesenchymal transition / kidney development / Deactivation of the beta-catenin transactivating complex / negative regulation of transforming growth factor beta receptor signaling pathway / Formation of the beta-catenin:TCF transactivating complex / brain development / fibrillar center / beta-catenin binding / histone binding / transcription by RNA polymerase II / transcription coactivator activity / chromatin binding / positive regulation of transcription by RNA polymerase II / nucleoplasm / nucleus / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Miller, T.C. / Rutherford, T.J. / Johnson, C.M. / Fiedler, M. / Bienz, M. | ||||||
![]() | ![]() Title: Allosteric Remodeling of the Histone H3 Binding Pocket in the Pygo2 Phd Finger Triggered by its Binding to the B9L/Bcl9 Co-Factor. Authors: Miller, T.C. / Rutherford, T.J. / Johnson, C.M. / Fiedler, M. / Bienz, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 96.5 KB | Display | ![]() |
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PDB format | ![]() | 74.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 455.2 KB | Display | ![]() |
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Full document | ![]() | 457.1 KB | Display | |
Data in XML | ![]() | 12.8 KB | Display | |
Data in CIF | ![]() | 17.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2vp7S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 11267.499 Da / Num. of mol.: 2 Fragment: PHD FINGER AND HD1 DOMAIN, RESIDUES 325-387,232-266 Source method: isolated from a genetically manipulated source Details: ARTIFICIAL LINKER BETWEEN PHD FINGER AND HD1 DOMAIN (7AA LINKER-GSGSGSG) Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-ZN / #3: Chemical | #4: Chemical | ChemComp-PEG / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.12 % / Description: NONE |
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Crystal grow | Details: 6% PEG 1000, 1.6 M (NH4)2SO4, 100 MM HEPES PH 7.5, 45 MM HCL |
-Data collection
Diffraction | Mean temperature: 77 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 29, 2009 / Details: UNDULATOR |
Radiation | Monochromator: SI(311) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.04 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→38.75 Å / Num. obs: 18032 / % possible obs: 99.5 % / Observed criterion σ(I): 1.5 / Redundancy: 3.4 % / Biso Wilson estimate: 17 Å2 / Rsym value: 0.08 / Net I/σ(I): 6.4 |
Reflection shell | Resolution: 1.9→2 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.358 / Mean I/σ(I) obs: 1.6 / Rsym value: 0.358 / % possible all: 99.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2VP7 Resolution: 1.9→31.31 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.945 / SU B: 4.873 / SU ML: 0.079 / Cross valid method: THROUGHOUT / ESU R: 0.139 / ESU R Free: 0.136 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.U VALUES RESIDUAL ONLY ATOM RECORD CONTAINS SUM OF TLS AND RESIDUAL B FACTORS. ANISOU RECORD CONTAINS SUM OF TLS AND RESIDUAL U FACTORS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 9.815 Å2
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Refinement step | Cycle: LAST / Resolution: 1.9→31.31 Å
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Refine LS restraints |
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