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- PDB-1arj: ARG-BOUND TAR RNA, NMR -

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Basic information

Entry
Database: PDB / ID: 1arj
TitleARG-BOUND TAR RNA, NMR
ComponentsTAR RNA
KeywordsRNA / NMR PEPTIDE-BOUND STRUCTURE / NUCLEIC ACIDS / COMPLEX (RNA-PEPTIDE)
Function / homologyARGININE / RNA / RNA (> 10)
Function and homology information
MethodSOLUTION NMR
AuthorsAboul-Ela, F. / Varani, G. / Karn, J.
Citation
Journal: J.Mol.Biol. / Year: 1995
Title: The structure of the human immunodeficiency virus type-1 TAR RNA reveals principles of RNA recognition by Tat protein.
Authors: Aboul-ela, F. / Karn, J. / Varani, G.
#1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1993
Title: Role of RNA Structure in Arginine Recognition of Tar RNA
Authors: Puglisi, J.D. / Chen, L. / Frankel, A.D. / Williamson, J.R.
#2: Journal: J.Mol.Biol. / Year: 1993
Title: Hydrogen-Bonding Contacts in the Major Groove are Required for Human Immunodeficiency Virus Type-1 Tat Protein Recognition of Tar RNA
Authors: Hamy, F. / Asseline, U. / Grasby, J. / Iwai, S. / Pritchard, C. / Slim, G. / Butler, P.J. / Karn, J. / Gait, M.J.
#3: Journal: J.Mol.Biol. / Year: 1993
Title: High Affinity Binding of Tar RNA by the Human Immunodeficiency Virus Type-1 Tat Protein Requires Base-Pairs in the RNA Stem and Amino Acid Residues Flanking the Basic Region
Authors: Churcher, M.J. / Lamont, C. / Hamy, F. / Dingwall, C. / Green, S.M. / Lowe, A.D. / Butler, J.G. / Gait, M.J. / Karn, J.
#4: Journal: Science / Year: 1992
Title: Conformation of the Tar RNA-Arginine Complex by NMR Spectroscopy
Authors: Puglisi, J.D. / Tan, R. / Calnan, B.J. / Frankel, A.D. / Williamson, J.R.
History
DepositionAug 30, 1995Processing site: BNL
Revision 1.0Nov 8, 1996Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 16, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
N: TAR RNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)9,4832
Polymers9,3081
Non-polymers1751
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / -
Representative

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Components

#1: RNA chain TAR RNA


Mass: 9307.555 Da / Num. of mol.: 1 / Source method: obtained synthetically
#2: Chemical ChemComp-ARG / ARGININE


Type: L-peptide linking / Mass: 175.209 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H15N4O2
Details: IDENTICAL, ACCORDING TO PRESENT KNOWLEDGE, TO ADP-1 (37 RESIDUE PEPTIDE) BOUND TAR RNA

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

Software
NameVersionClassification
X-PLOR3.1model building
X-PLOR3.1refinement
X-PLOR3.1phasing
NMR softwareName: X-PLOR / Version: 3.1 / Developer: BRUNGER / Classification: refinement
NMR ensembleConformers submitted total number: 20

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