- PDB-2wys: High resolution crystallographic structure of the Clostridium the... -
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Basic information
Entry
Database: PDB / ID: 2wys
Title
High resolution crystallographic structure of the Clostridium thermocellum N-terminal endo-1,4-beta-D-xylanase 10B (Xyn10B) CBM22-1- GH10 modules complexed with xylohexaose
SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AF" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AF" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "BE" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
Mass: 18.015 Da / Num. of mol.: 378 / Source method: isolated from a natural source / Formula: H2O
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Details
Compound details
ENGINEERED RESIDUE IN CHAIN A, GLU 337 TO ALA ENGINEERED RESIDUE IN CHAIN B, GLU 337 TO ALA
Nonpolymer details
POTASSIUM (K): FROM THE CRYSTALLIZATION BUFFER PHOSPHATE ION (PO4): FRO THE CRYSTALLIZATION BUFFER ...POTASSIUM (K): FROM THE CRYSTALLIZATION BUFFER PHOSPHATE ION (PO4): FRO THE CRYSTALLIZATION BUFFER 2 XYLOHEXOSES (BUT ONLY 3 OF THE 6 XYLOSE RINGS CAN BE SEEN IN THE ELECTRON DENSITY), 2 PHOSPHATE AND 4 POTASSIUM IONS.
Sequence details
THE PROTEIN SEQUENCE OF THE CONSTRUCT CORRESPONDS TO AMINO ACID RESIDUES 32 TO 551 WITH THE E337A ...THE PROTEIN SEQUENCE OF THE CONSTRUCT CORRESPONDS TO AMINO ACID RESIDUES 32 TO 551 WITH THE E337A MUTATION AND AN ADDITIONAL TWENTY AMINO ACID RESIDUES AT THE N-TERMINUS, MGSSHHHHHHSSGLVPRGSH. LINKER REGION BETWEEN AMINO ACID RESIDUES A182 TO A188 AND B182 AND B185 ARE NOT SEEN IN THE ELECTRON DENSITY MAPS.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 4.9 Å3/Da / Density % sol: 75 % Description: PEAK DATA WAS THE BEST DATA COLLECTED OVER THE THREE WAVELENGTHS AND WAS USED FOR MODELING THE STRUCTURE