+Open data
-Basic information
Entry | Database: PDB / ID: 2wuk | ||||||
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Title | DivIVA N-terminal domain, F17A mutant | ||||||
Components | SEPTUM SITE-DETERMINING PROTEIN DIVIVA | ||||||
Keywords | CELL CYCLE / BACTERIAL CELL DIVISION / SEPTATION / SPORULATION | ||||||
Function / homology | Function and homology information division septum assembly / sporulation resulting in formation of a cellular spore / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | BACILLUS SUBTILIS (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Oliva, M.A. / Leonard, T.A. / Lowe, J. | ||||||
Citation | Journal: Embo J. / Year: 2010 Title: Features Critical for Membrane Binding Revealed by Diviva Crystal Structure. Authors: Oliva, M.A. / Halbedel, S. / Freund, S.M. / Dutow, P. / Leonard, T.A. / Veprintsev, D.B. / Hamoen, L.W. / Lowe, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2wuk.cif.gz | 60.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2wuk.ent.gz | 49.7 KB | Display | PDB format |
PDBx/mmJSON format | 2wuk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wu/2wuk ftp://data.pdbj.org/pub/pdb/validation_reports/wu/2wuk | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
#1: Protein | Mass: 6762.587 Da / Num. of mol.: 4 / Fragment: N-TERMINAL DOMAIN, RESIDUES 1-57 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) BACILLUS SUBTILIS (bacteria) / Strain: 168 / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P71021 #2: Water | ChemComp-HOH / | Compound details | ENGINEERED RESIDUE IN CHAIN A, PHE 17 TO ALA ENGINEERED RESIDUE IN CHAIN B, PHE 17 TO ALA ...ENGINEERED | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 36.2 % / Description: NONE |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU300 / Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Feb 1, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→21.3 Å / Num. obs: 14059 / % possible obs: 96.7 % / Observed criterion σ(I): 0 / Redundancy: 2.8 % / Biso Wilson estimate: 16.85 Å2 / Rmerge(I) obs: 0.05 / Net I/σ(I): 13.3 |
Reflection shell | Resolution: 1.9→2 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.2 / Mean I/σ(I) obs: 5.2 / % possible all: 96.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.9→15.551 Å / SU ML: 0.8 / σ(F): 0.96 / Phase error: 23.45 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 60.14 Å2 / ksol: 0.384 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.9→15.551 Å
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Refine LS restraints |
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LS refinement shell |
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