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Open data
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Basic information
Entry | Database: PDB / ID: 2wli | ||||||
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Title | POTASSIUM CHANNEL FROM MAGNETOSPIRILLUM MAGNETOTACTICUM | ||||||
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![]() | METAL TRANSPORT / INTEGRAL MEMBRANE PROTEIN / IONIC CHANNEL / ION TRANSPORT / TRANSPORT | ||||||
Function / homology | ![]() inward rectifier potassium channel activity / regulation of monoatomic ion transmembrane transport / monoatomic ion channel complex / potassium ion import across plasma membrane / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Clarke, O.B. / Caputo, A.T. / Smith, B.J. / Gulbis, J.M. | ||||||
![]() | ![]() Title: Domain Reorientation and Rotation of an Intracellular Assembly Regulate Conduction in Kir Potassium Channels. Authors: Clarke, O.B. / Caputo, A.T. / Hill, A.P. / Vandenberg, J.I. / Smith, B.J. / Gulbis, J.M. | ||||||
History |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 225.8 KB | Display | ![]() |
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PDB format | ![]() | 182.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2wlhC ![]() 2wljC ![]() 2wlkC ![]() 2wllC ![]() 2wlmC ![]() 2wlnC ![]() 2wloC ![]() 2x6aC ![]() 2x6bC ![]() 2x6cC ![]() 1xl4S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 33826.801 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: C-TERMINAL HEXAHISTIDINE TAG APPENDED Source: (gene. exp.) ![]() Description: EXPRESSED RECOMBINANTLY IN ESCHERICHIA COLI / Production host: ![]() ![]() | ||||
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#2: Protein | Mass: 33782.766 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: C-TERMINAL HEXAHISTIDINE TAG APPENDED Source: (gene. exp.) ![]() Description: EXPRESSED RECOMBINANTLY IN ESCHERICHIA COLI / Production host: ![]() ![]() | ||||
#3: Chemical | ChemComp-K / #4: Water | ChemComp-HOH / | Sequence details | RESIDUES 5-295 CORRESPOND | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.44 Å3/Da / Density % sol: 72 % / Description: NONE |
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Crystal grow | Method: vapor diffusion, sitting drop / pH: 7.5 Details: PRECIPITANT: 15 % PEG 400 2.5 % PEG 4000 2.5 % PEG 8000 10 % GLYCEROL 90 MM HEPES PH 7.5 PROTEIN: 8MG/ML KIRBAC3.1 150MM KCL 20MM TRIS8.0 0.05% TDM 4MM LDAO PROTEIN AND PRECIPITANT WERE ...Details: PRECIPITANT: 15 % PEG 400 2.5 % PEG 4000 2.5 % PEG 8000 10 % GLYCEROL 90 MM HEPES PH 7.5 PROTEIN: 8MG/ML KIRBAC3.1 150MM KCL 20MM TRIS8.0 0.05% TDM 4MM LDAO PROTEIN AND PRECIPITANT WERE COMBINED IN A 1:1 RATIO AND EQUILIBRATED AGAINST A RESERVOIR OF PRECIPITANT IN A SITTING-DROP VAPOUR-DIFFUSION SET-UP. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: May 18, 2008 / Details: MIRRORS |
Radiation | Monochromator: SILICON CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.953639 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→50 Å / Num. obs: 20089 / % possible obs: 100 % / Observed criterion σ(I): -3 / Redundancy: 7.9 % / Biso Wilson estimate: 0 Å2 / Rmerge(I) obs: 0.13 / Net I/σ(I): 16.6 |
Reflection shell | Resolution: 3.1→3.21 Å / Redundancy: 8.1 % / Rmerge(I) obs: 0.64 / Mean I/σ(I) obs: 3.5 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1XL4 Resolution: 3.09→15 Å / Cor.coef. Fo:Fc: 0.876 / Cor.coef. Fo:Fc free: 0.841 / SU B: 47.031 / SU ML: 0.378 / Cross valid method: THROUGHOUT / ESU R: 1.322 / ESU R Free: 0.432 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES RESIDUAL ONLY
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Solvent computation | Ion probe radii: 1 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 32.917 Å2
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Refinement step | Cycle: LAST / Resolution: 3.09→15 Å
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Refine LS restraints |
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