[English] 日本語
![](img/lk-miru.gif)
- PDB-1xl4: Intermediate gating structure 1 of the inwardly rectifying K+ cha... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1xl4 | ||||||
---|---|---|---|---|---|---|---|
Title | Intermediate gating structure 1 of the inwardly rectifying K+ channel KirBac3.1 | ||||||
![]() | Inward rectifier potassium channel | ||||||
![]() | METAL TRANSPORT / integral membrane protein / ion channel / inwardly rectifying K+ channel | ||||||
Function / homology | ![]() inward rectifier potassium channel activity / regulation of monoatomic ion transmembrane transport / membrane => GO:0016020 / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Gulbis, J.M. / Kuo, A. / Smith, B. / Doyle, D.A. / Edwards, A. / Arrowsmith, C. / Sundstrom, M. | ||||||
![]() | ![]() Title: Two intermediate gating state crystal structures of the KirBac3.1 K+ channel Authors: Gulbis, J.M. / Kuo, A. / Smith, B. / Doyle, D.A. / Edwards, A. / Arrowsmith, C. / Sundstrom, M. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 127.7 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 99.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 443.7 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 457.2 KB | Display | |
Data in XML | ![]() | 25 KB | Display | |
Data in CIF | ![]() | 34.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1xl6C ![]() 1p7bS S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||||||||||||||||||||||||
Unit cell |
| ||||||||||||||||||||||||||||||
Components on special symmetry positions |
|
-
Components
#1: Protein | Mass: 33782.766 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Plasmid: pET-30a / Production host: ![]() ![]() #2: Chemical | ChemComp-K / #3: Chemical | ChemComp-MG / | #4: Water | ChemComp-HOH / | Nonpolymer details | THERE ARE TWO ALTERNATE ARRANGEMENTS OF IONS AND COORDINATED WATER MOLECULES. THE FIRST INCLUDES ...THERE ARE TWO ALTERNATE ARRANGEMEN | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 3.8 Å3/Da / Density % sol: 65 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.6 Details: 90mM Hepes, 2.5% PEG 8000, 2.5% PEG 4000, 20% PEG 400, 12.5mM magnesium chloride, 42.5mM magnesium acetate, 2.5% glycerol, 14mM Hega-10, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
---|---|
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.6→26 Å / Num. obs: 29551 |
-
Processing
Software |
| ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1P7B Resolution: 2.6→12 Å / Cross valid method: Brunger / σ(F): 3
| ||||||||||||||||||||||||||||
Displacement parameters |
| ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→12 Å
| ||||||||||||||||||||||||||||
Refine LS restraints |
|