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- PDB-1xl6: Intermediate gating structure 2 of the inwardly rectifying K+ cha... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1xl6 | ||||||
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Title | Intermediate gating structure 2 of the inwardly rectifying K+ channel KirBac3.1 | ||||||
![]() | Inward rectifier potassium channel | ||||||
![]() | METAL TRANSPORT / integral membrane protein / ion channel / inwardly rectifying K+ channel | ||||||
Function / homology | ![]() inward rectifier potassium channel activity / regulation of monoatomic ion transmembrane transport / monoatomic ion channel complex / potassium ion import across plasma membrane / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Gulbis, J.M. / Kuo, A. / Smith, B. / Doyle, D.A. / Edwards, A. / Arrowsmith, C. / Sundstrom, M. | ||||||
![]() | ![]() Title: Two intermediate gating state crystal structures of the KirBac3.1 K+ channel Authors: Gulbis, J.M. / Kuo, A. / Smith, B. / Doyle, D.A. / Edwards, A. / Arrowsmith, C. / Sundstrom, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 129.9 KB | Display | ![]() |
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PDB format | ![]() | 102.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 454.6 KB | Display | ![]() |
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Full document | ![]() | 488.6 KB | Display | |
Data in XML | ![]() | 27.1 KB | Display | |
Data in CIF | ![]() | 36.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1xl4C ![]() 1p7bS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 33782.766 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Plasmid: pET-30a / Production host: ![]() ![]() #2: Chemical | ChemComp-K / #3: Chemical | ChemComp-MG / | #4: Chemical | ChemComp-SPM / | #5: Water | ChemComp-HOH / | Nonpolymer details | THERE ARE THREE ALTERNATE ARRANGEMENTS OF IONS AND COORDINATED WATER MOLECULES. THEY ARE NOT ...THERE ARE THREE ALTERNATE ARRANGEMEN | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.77 Å3/Da / Density % sol: 65 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.6 Details: 90mM Hepes, 2.5% PEG 8000, 2.5% PEG 4000, 20% PEG 400, 12.5mM magnesium chloride, 42.5mM magnesium acetate, 2.5% glycerol, 14mM Hega-10 , pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.7→30 Å / Num. obs: 28510 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1P7B Resolution: 2.85→10 Å / σ(F): 3
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Refinement step | Cycle: LAST / Resolution: 2.85→10 Å
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