- PDB-2r6g: The Crystal Structure of the E. coli Maltose Transporter -
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基本情報
登録情報
データベース: PDB / ID: 2r6g
タイトル
The Crystal Structure of the E. coli Maltose Transporter
要素
(Maltose transport system permease protein ...) x 2
Maltose-binding periplasmic protein
Maltose/maltodextrin import ATP-binding protein malK
キーワード
HYDROLASE/TRANSPORT PROTEIN / ABC transporter / catalytic intermediate / E. coli maltose transporter / MBP / maltodextrin binding protein / MalK / ATP binding cassette / ATP-binding / Hydrolase / Inner membrane / Membrane / Nucleotide-binding / Sugar transport / Transmembrane / HYDROLASE-TRANSPORT PROTEIN COMPLEX
機能・相同性
機能・相同性情報
ABC-type maltose transporter / ABC-type maltose transporter activity / negative regulation of maltose transport / enzyme IIA-maltose transporter complex / negative regulation of transmembrane transport / detection of maltose stimulus / maltose transport complex / carbohydrate transport / carbohydrate transmembrane transporter activity / maltose binding ...ABC-type maltose transporter / ABC-type maltose transporter activity / negative regulation of maltose transport / enzyme IIA-maltose transporter complex / negative regulation of transmembrane transport / detection of maltose stimulus / maltose transport complex / carbohydrate transport / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / ATP-binding cassette (ABC) transporter complex / cell chemotaxis / outer membrane-bounded periplasmic space / DNA-binding transcription factor binding / periplasmic space / DNA damage response / ATP hydrolysis activity / ATP binding / membrane / plasma membrane 類似検索 - 分子機能
MalF N-terminal region-like / Periplasmic binding protein-like II / D-maltodextrin-binding protein, MBP / MalF N-terminal region-like / MalF N-terminal region-like / Maltose transport system permease protein MalF, P2 domain / MalF, N-terminal / : / : / Maltose transport system permease protein MalF P2 domain ...MalF N-terminal region-like / Periplasmic binding protein-like II / D-maltodextrin-binding protein, MBP / MalF N-terminal region-like / MalF N-terminal region-like / Maltose transport system permease protein MalF, P2 domain / MalF, N-terminal / : / : / Maltose transport system permease protein MalF P2 domain / MalF, N-terminal region, transmembrane helices / Maltose/Maltodextrin import ATP-binding protein malK family profile. / MetI-like fold / MetI-like / Transport-associated OB, type 2 / TOBE domain / : / MalK OB fold domain / ABC transporter, maltose/maltodextrin import, MalK-like / : / Molybdate/tungstate binding, C-terminal / ABC transporter type 1, transmembrane domain MetI-like / MetI-like superfamily / Binding-protein-dependent transport system inner membrane component / ABC transporter integral membrane type-1 domain profile. / RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain / Maltose/Cyclodextrin ABC transporter, substrate-binding protein / Solute-binding family 1, conserved site / Bacterial extracellular solute-binding proteins, family 1 signature. / Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein / Nucleic acid-binding proteins / Methane Monooxygenase Hydroxylase; Chain G, domain 1 / Periplasmic binding protein-like II / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / D-Maltodextrin-Binding Protein; domain 2 / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / P-loop containing nucleotide triphosphate hydrolases / Nucleic acid-binding, OB-fold / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Roll / Up-down Bundle / Beta Barrel / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Beta / Mainly Alpha / Alpha Beta 類似検索 - ドメイン・相同性
alpha-maltose / ADENOSINE-5'-TRIPHOSPHATE / Maltose/maltodextrin transport system permease protein MalF / Maltose/maltodextrin-binding periplasmic protein / Maltose/maltodextrin transport system permease protein MalG / Maltose/maltodextrin import ATP-binding protein MalK / Maltose/maltodextrin import ATP-binding protein MalK 類似検索 - 構成要素
A: Maltose/maltodextrin import ATP-binding protein malK B: Maltose/maltodextrin import ATP-binding protein malK E: Maltose-binding periplasmic protein F: Maltose transport system permease protein malF G: Maltose transport system permease protein malG ヘテロ分子
モノクロメーター: Double crystal cryo-cooled Si(111) プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.0332 Å / 相対比: 1
Reflection
冗長度: 3.6 % / Av σ(I) over netI: 8.3 / 数: 236250 / Rmerge(I) obs: 0.092 / Χ2: 1.09 / D res high: 2.8 Å / D res low: 50 Å / Num. obs: 66520 / % possible obs: 91.4
解像度: 2.8→47.4 Å / Cor.coef. Fo:Fc: 0.922 / Cor.coef. Fo:Fc free: 0.899 / SU B: 40.785 / SU ML: 0.361 / 交差検証法: THROUGHOUT / ESU R Free: 0.408 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.2714
1185
2 %
RANDOM
Rwork
0.24164
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obs
0.24223
58103
83.76 %
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all
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59288
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK