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- PDB-2r4n: Crystal structure of the long-chain fatty acid transporter FadL m... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2r4n | ||||||
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Title | Crystal structure of the long-chain fatty acid transporter FadL mutant N33A | ||||||
![]() | Long-chain fatty acid transport protein | ||||||
![]() | TRANSPORT PROTEIN / beta barrel / outer membrane protein / Lipid transport / Phage recognition / Transmembrane / Transport | ||||||
Function / homology | ![]() long-chain fatty acid transporting porin activity / ligand-gated channel activity / cell outer membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hearn, E.M. / Patel, D.R. / Lepore, B.W. / Indic, M. / van den Berg, B. | ||||||
![]() | ![]() Title: Transmembrane passage of hydrophobic compounds through a protein channel wall Authors: Hearn, E.M. / Patel, D.R. / Lepore, B.W. / Indic, M. / van den Berg, B. #1: ![]() Title: Crystal structure of the long-chain fatty acid transporter FadL Authors: van den Berg, B. / Black, P.N. / Clemons Jr., W.M. / Rapoport, T.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 157.4 KB | Display | ![]() |
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PDB format | ![]() | 127.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 450.5 KB | Display | ![]() |
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Full document | ![]() | 499.3 KB | Display | |
Data in XML | ![]() | 35.8 KB | Display | |
Data in CIF | ![]() | 48.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2r4lC ![]() 2r4oC ![]() 2r4pC ![]() 2r88C ![]() 3dwnC ![]() 3dwoC ![]() 1t16S ![]() 2r4m S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 46711.918 Da / Num. of mol.: 2 / Mutation: N33A, I197T Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.68 Å3/Da / Density % sol: 54.04 % |
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Crystal grow | Temperature: 295 K / pH: 4.6 Details: 30% PEG 400, 0.1M cadmium chloride, 0.1M sodium acetate, pH 4.60, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Apr 4, 2006 |
Radiation | Monochromator: SI(111) CHANNEL CUT MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 3.11→37.12 Å / Num. obs: 15295 / % possible obs: 85.1 % / Observed criterion σ(I): 2 / Redundancy: 2.9 % / Rmerge(I) obs: 0.126 / Net I/σ(I): 6.8 |
Reflection shell | Resolution: 3.11→3.21 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.288 / Mean I/σ(I) obs: 3.78 / Rsym value: 0.288 / % possible all: 76.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1T16 Resolution: 3.2→10 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: ENGH & HUBER
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Displacement parameters | Biso mean: 22.97 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.2→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.2→3.34 Å / Rfactor Rfree error: 0.034
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